Gene deletion from Plasmodium falciparum using FLP and Cre recombinases: Implications for applied site-specific recombination

2011 ◽  
Vol 41 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Matthew T. O’Neill ◽  
Thuan Phuong ◽  
Julie Healer ◽  
Dave Richard ◽  
Alan F. Cowman
Keyword(s):  
Plasmodium Falciparum ◽  
Gene Deletion ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Employing Site-Specific Recombination for Conditional Genetic Analysis in Sinorhizobium meliloti

2011 ◽  
Vol 77 (12) ◽  
pp. 3916-3922 ◽  
Author(s):  
Clarice L. Harrison ◽  
Matthew B. Crook ◽  
Gledi Peco ◽  
Sharon R. Long ◽  
Joel S. Griffitts
Keyword(s):  
Temporal Resolution ◽  
Sinorhizobium Meliloti ◽  
Gene Deletion ◽  
Colorimetric Assay ◽  
Bacterial Species ◽  
Site Specific ◽  
Content Type ◽  
Site Specific Recombination ◽  
Or Gene ◽  
Or Genes

ABSTRACTThe ability to remove a genetic function from an organism with good temporal resolution is crucial for characterizing essential genes or genes that act in complex developmental programs. The rhizobium-legume symbiosis involves an elaborate two-organism interaction requiring multiple levels of signal exchange. As an important step toward probing rhizobium genetic functions with temporal resolution, we present the development of a conditional gene deletion system inSinorhizobium melilotithat employs Cre/loxPsite-specific recombination. This system enables chemically inducible and irreversible gene deletion or gene upregulation. Recombinase-mediated excision events can be positively or negatively selected or monitored by a colorimetric assay. The system may be adaptable to various bacterial species, in which recombinase activity may be placed under the control of diverse user-defined promoters. This system also shows promise for uses in promoter trapping and biosensing applications.

Progress on XerCD/dif site-specific recombination

2012 ◽  
Vol 34 (8) ◽  
pp. 1003-1008
Author(s):  
De-Qiao TIAN ◽  
Yu-Min WANG ◽  
Tao ZHENG
Keyword(s):  
Site Specific ◽  
Site Specific Recombination
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