Effect of Neutralization of Gastric Aspirate on Culture Yield of Mycobacterium Tuberculosis in Children with Pulmonary Tuberculosis

Abstract Objective Diagnosis of pulmonary tuberculosis (PTB) in pediatrics is a challenge due to the paucibacillary condition of the disease in this population, low sputum expectoration, and diverse unspecific symptomatology. Mycobacterial isolation through culture remains a priority. The objective of this study is to explore gastric aspirates and induced sputum techniques in the pediatric population for positivity on mycobacterial cultures. Methods In this observational analytical study, two temporal groups were evaluated. A comparison of the isolation rate defined as positive culture confirmation by gastric aspirate (GA), induced sputum (IS), or combination of these both techniques in children under 10 years of age. The study included 86 children, 37 in the first evaluated study group and 49 in the second group. Discussion Culture positivity was 10.8 and 30.6% for the first and second case series, respectively. These findings showed that the combination of GA and IS in two consecutive days yielded a significantly higher detection rate to confirm pulmonary tuberculosis by culture. Conclusion The combination of GA plus IS samples for collection of M. tuberculosis culture can be a useful, nonexpensive, and safe diagnostic tool in low- and middleincome countries to diagnose tuberculosis in children.

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Please, No More Gastric Aspirate to Diagnose Pulmonary Tuberculosis in Children

Clinical Infectious Diseases ◽

10.1093/cid/cix712 ◽

2017 ◽

Vol 65 (12) ◽

pp. 2158-2158 ◽

Cited By ~ 4

Author(s):

Michel Drancourt

Keyword(s):

Pulmonary Tuberculosis ◽

Gastric Aspirate ◽

Tuberculosis In Children

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Effective use of Penicillin to Improve Culture Yield for Mycobacterium tuberculosis

Tribhuvan University Journal of Microbiology ◽

10.3126/tujm.v6i0.26592 ◽

2019 ◽

Vol 6 ◽

pp. 103-107

Author(s):

Sneha Pradhan ◽

Gokarna Raj Ghimire ◽

Shova Shrestha

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Sputum Sample ◽

Early Morning ◽

National Tuberculosis ◽

Effective Use ◽

Culture Yield

Objective: To compare LJ media and LJ media with penicillin for the growth of Mycobacterium tuberculosis and contamination, in pulmonary tuberculosis (PTB) suspected patients. Methods: A total of 300 PTB suspected cases at National Tuberculosis center (NTC) for analyzed for culture and contamination. Early morning sputum samples were collected in sterile leak-proof falcon tube. Digestion, decontamination and homogenization of sputum were done using NALC-NaOH (Modified Petroff method). The sputum sample was processed on LJ media and penicillin added LJ media and incubated at 37. Cultures were examined after 8 weeks. Results: All the PTB suspected cases were compared in LJ media and LJ media with penicillin, 29.7% (89) were positive, 21% (63) were contaminated on LJ media whereas 41% (123) were positive, 3.7 % (11) were contaminated on penicillin added LJ media. Also, 25 (8%) were 1+ grading, 14 (4.7%) were 2+ grading, whereas 81 (27%) and 45 (15%) were 3+ grading LJ + Penicillin and LJ media respectively. Conclusion: Contamination was reduced by 17.3% with the addition of penicillin to LJ media. And isolation of total positive cultures was enhanced by 11.3%.

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Diagnosis of Pulmonary Tuberculosis in Children

Paediatrica Indonesiana ◽

10.14238/pi15.11-12.1975.286-96 ◽

2017 ◽

Vol 15 (11-12) ◽

pp. 286

Author(s):

Gunadi Santosa

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Clinical Diagnosis ◽

Gastric Lavage ◽

Tuberculin Test ◽

Medical Doctor ◽

Definite Diagnosis ◽

Bacteriological Diagnosis ◽

The Moment ◽

Tuberculosis In Children

1. A definite diagnosis of tuberculosis can only be established on the basis of the finding of the tubercle bacilli.2. The isolation of the mycobacterium tuberculosis from a child is subject to difficulties:a. the necessity of gastric lavage for 3 consecutive days, which is difficult to perform ambulatory.b. the necessity of the proper facilities of a microbiologic laboratory.c. the outcome is often not sufficiently high.3. It is concluded that at the moment making of the bacteriological diagnosis of child tuberculosis is not practical, so that we are forced to depend on the clinical diagnosis.4. The tuberculin test is very important in establishing the diagnosis. It is recommended to perform this test routinely on every child once a year and also that every medical doctor as well as specialist performs this test to detect a case of TBC at the earliest possible moment.

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Diagnostic value of certain methods for isolation of Mycobacterium tuberculosis in children with suspected pulmonary tuberculosis

Medicinski pregled ◽

10.2298/mpns0506231p ◽

2005 ◽

Vol 58 (5-6) ◽

pp. 231-235 ◽

Cited By ~ 4

Author(s):

Slobodanka Petrovic

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Gastric Lavage ◽

Lavage Fluid ◽

Diagnostic Value ◽

Bacteriological Diagnosis ◽

Tuberculosis In Children ◽

Better Than

Introduction The aim of this study was to find out if bronchoalveolar lavage (BL) is better than gastric lavage (GL) or sputum (SP) examination for isolation of Mycobacterium tuberculosis in childhood pulmonary tuberculosis. Material and methods The study included a group of 30 children with suspected pulmonary tuberculosis, aged 6 months to 18 years. Gastric lavage was done on 4 consecutive mornings after overnight fast. Sputum was examined in 12 patients older than 10 years, (4 consecutive samples). BL was performed on the same day as the last GL and SP, using a rigid bronchoscope. Specimens were examined for the existence of BL acid fast bacilli (AFB) and culture for Mycobacterium tb. Results Out of 30 cases, Mycobacterium tb was grown in 13 BL samples (43.33%), and in 10 GL samples from the same patients (33.33%). Comparative analysis of microbiological samples of sputum and bronchoalveolar lavage fluid in 12 children showed that 6 patients had SP+ cultures and 6 patients had SP-cultures of Mycobacterium tb. All SP+ patients where also BL+, but 1 SP- patient was BL+. Smear examination of BL fluid samples was positive for AFB in 10 patients with positive cultures for Mycobacterium tb. Conclusion The results of our study indicate that BL examination is better than GL and SP examination for bacteriological diagnosis of childhood tuberculosis. .

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Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v6i2.19368 ◽

2012 ◽

Vol 6 (2) ◽

pp. 2-6 ◽

Cited By ~ 1

Author(s):

Mohammad Jobayer ◽

SM Shamsuzzaman ◽

Kazi Zulfiquer Mamun

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Accurate Method ◽

Major Health Problem ◽

Major Health ◽

Tuberculosis Patients ◽

Diagnostic Potential ◽

Total Death ◽

Smear Negative ◽

Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

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