Dilute phosphoric acid pretreatment of wheat bran for enzymatic hydrolysis and subsequent ethanol production by edible fungi Neurospora intermedia

Abstract Quercus infectoria is one of the most abundant native oak species in the Kurdistan region of Iraq. This study focused on utilizing leaves of Quercus infectoria for ethanol production in the region. A typical three-step conversion process of acid pretreatment, enzymatic hydrolysis, and yeast fermentation was investigated to produce ethanol from the leaves. Under the selected acid pretreatment and enzymatic hydrolysis conditions, the glucose and xylose concentrations in the hydrolysates reached 11.4 g/L and 16.8 g/L, respectively, with the corresponding sugar conversions of 42.8% and 99.8%. A yeast strain, Kluyveromyces marxianus, was used to ferment mono-sugars in the hydrolysates for ethanol production. The ethanol production rate and conversion of K. marxianus in the fermentation were 0.17 g/L/h and 27%. The techno-economic analysis further concluded that a regional ethanol biorefinery can be established in the Zawita sub-district, Iraq to utilize Q. infectoria leaves to produce 200,000,000 kg ethanol/year with a positive energy balance of 745,052,623 MJ/year. The net annual revenue of the biorefinery is $123,692,804. The payback period of the biorefinery is 10 years.

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Effect of phosphoric acid pretreatment on enzymatic hydrolysis of microcrystalline cellulose

Biotechnology Advances ◽

10.1016/j.biotechadv.2010.05.010 ◽

2010 ◽

Vol 28 (5) ◽

pp. 613-619 ◽

Cited By ~ 43

Author(s):

Juanhua Zhang ◽

Beixiao Zhang ◽

Jingqiang Zhang ◽

Lu Lin ◽

Shijie Liu ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Phosphoric Acid ◽

Microcrystalline Cellulose ◽

Acid Pretreatment ◽

Hydrolysis Of

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Valorization of olive stones for xylitol and ethanol production from dilute acid pretreatment via enzymatic hydrolysis and fermentation by Pachysolen tannophilus

Biochemical Engineering Journal ◽

10.1016/j.bej.2014.06.023 ◽

2014 ◽

Vol 90 ◽

pp. 286-293 ◽

Cited By ~ 21

Author(s):

Marwa Saleh ◽

Manuel Cuevas ◽

Juan F. García ◽

Sebastián Sánchez

Keyword(s):

Enzymatic Hydrolysis ◽

Ethanol Production ◽

Dilute Acid Pretreatment ◽

Dilute Acid ◽

Acid Pretreatment ◽

Pachysolen Tannophilus ◽

Olive Stones

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Fermentable Sugar Production from the Peels of Two Durian (Durio zibethinus Murr.) Cultivars by Phosphoric Acid Pretreatment

Resources ◽

10.3390/resources7040060 ◽

2018 ◽

Vol 7 (4) ◽

pp. 60 ◽

Cited By ~ 5

Author(s):

Abraham Obeng ◽

Duangporn Premjet ◽

Siripong Premjet

Keyword(s):

Enzymatic Hydrolysis ◽

Phosphoric Acid ◽

Glucose Concentration ◽

Crystallinity Index ◽

Xrd Analysis ◽

X Ray Diffraction ◽

Acid Pretreatment ◽

Durio Zibethinus ◽

Hydrolysis Process ◽

Acid Soluble Lignin

The potential of durian (Durio zibethinus Murr.) peel as feedstock for the production of fermentable sugars was evaluated. Durian peel biomass from two cultivars, monthong (Durio zibethinus Murr. cv. Monthong) and chanee (Durio zibethinus Murr. cv. Chanee), were pretreated with different concentrations (70%, 75%, 80%, and 85%) of phosphoric acid (H3PO4) at a moderate temperature of 60 °C for 60 min. The H3PO4-pretreated durian peel biomass was then subjected to enzymatic hydrolysis. Significantly higher glucan (44.74 ± 0.21%) content was observed in the monthong peel compared to the chanee peel (42.06 ± 0.28%). Phosphoric acid pretreatment caused the significant solubilization of the xylan and acid soluble lignin (ASL) contents. This enhanced the enzymatic hydrolysis process causing a significant increase in the hydrolysis efficiency and glucose concentration. The highest hydrolysis efficiency and glucose concentration were obtained after 72 h from the 75% H3PO4-pretreated peel biomass for both the monthong (90.33 ± 0.42% and 9.55 ± 0.11 g/L, respectively) and chanee (90.06 ± 0.40% and 8.56 ± 0.13 g/L, respectively) peels. Biomass to glucose recovery for monthong and chanee were improved by approximately 7- and 6-fold, respectively. The scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis showed destruction of the peel biomass structure and changes in the cellulose crystallinity index (CrIs).

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