Chemical composition, antioxidant, anti-inflammatory, anti-microbial and in-vitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: An endangered medicinal plant of North East India

2019 ◽  
Vol 129 ◽  
pp. 448-454 ◽  
Author(s):  
Angana Borah ◽  
Manabi Paw ◽  
Roktim Gogoi ◽  
Rikraj Loying ◽  
Neelav Sarma ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Medicinal Plant ◽  
North East India ◽  
North East ◽  
Endangered Medicinal Plant ◽  
Anti Inflammatory

Comparative Analysis of In-Vitro Biological Activities of Methyl Eugenol Rich Cymbopogon khasianus Hack., Leaf Essential Oil with Pure Methyl Eugenol Compound

2020 ◽  
Vol 21 (10) ◽  
pp. 927-938 ◽  
Author(s):  
Roktim Gogoi ◽  
Rikraj Loying ◽  
Neelav Sarma ◽  
Twahira Begum ◽  
Sudin K. Pandey ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
Methyl Eugenol ◽  
Dpph Assay ◽  
Pure Compound ◽  
Allium Cepa Assay ◽  
Anti Inflammatory ◽  
Lemongrass Essential Oil

Background: The essential oil of methyl eugenol rich Cymbopogon khasianus Hack. was evaluated and its bioactivities were compared with pure methyl eugenol. So far, methyl eugenol rich essential oil of lemongrass was not studied for any biological activities; hence, the present study was conducted. Objective: This study examined the chemical composition of essential oil of methyl eugenol rich Cymbopogon khasianus Hack., and evaluated its antioxidant, anti-inflammatory, antimicrobial, and herbicidal properties and genotoxicity, which were compared with pure compound, methyl eugenol. Material and Methods: Methyl eugenol rich variety of Cymbopogon khasianus Hack., with registration no. INGR18037 (c.v. Jor Lab L-9) was collected from experimental farm CSIR-NEIST, Jorhat, Assam (26.7378°N, 94.1570°E). The essential oil wasobtained by hydro-distillation using a Clevenger apparatus. The chemical composition of the essential oil was evaluated using GC/MS analysis and its antioxidant (DPPH assay, reducing power assay), anti-inflammatory (Egg albumin denaturation assay), and antimicrobial (Disc diffusion assay, MIC) properties, seed germination effect and genotoxicity (Allium cepa assay) were studied and compared with pure Methyl Eugenol compound (ME). Results: Major components detected in the Essential Oil (EO) through Gas chromatography/mass spectroscopy analysis were methyl eugenol (73.17%) and β-myrcene (8.58%). A total of 35components were detected with a total identified area percentage of 98.34%. DPPH assay revealed considerable antioxidant activity of methyl eugenol rich lemongrass essential oil (IC50= 2.263 μg/mL), which is lower than standard ascorbic acid (IC50 2.58 μg/mL), and higher than standard Methyl Eugenol (ME) (IC50 2.253 μg/mL). Methyl eugenol rich lemongrass EO showed IC50 38.00 μg/mL, ME 36.44 μg/mL, and sodium diclofenac 22.76 μg/mL, in in-vitro anti-inflammatory test. Moderate antimicrobial activity towards the 8 tested microbes was shown by methyl eugenol rich lemongrass essential oil whose effectiveness against the microbes was less as compared to pure ME standard. Seed germination assay further revealed the herbicidal properties of methyl eugenol rich essential oil. Moreover, Allium cepa assay revealed moderate genotoxicity of the essential oil. Conclusion: This paper compared the antioxidant, anti-inflammatory, antimicrobial, genotoxicity and herbicidal activities of methyl eugenol rich lemongrass with pure methyl eugenol. This methyl eugenol rich lemongrass variety can be used as an alternative of methyl eugenol pure compound. Hence, the essential oil of this variety has the potential of developing cost-effective, easily available antioxidative/ antimicrobial drugs but its use should be under the safety range of methyl eugenol and needs further clinical trials.

A comparative analysis of bark and leaf essential oil and their chemical composition, antioxidant, anti-inflammatory, antimicrobial activities and genotoxic potential of North East Indian Cinnamomum zeylanicum Blume

2019 ◽  
Vol 09 ◽  
Author(s):  
Roktim Gogoi ◽  
Neelav Sarma ◽  
Rikraj Loying ◽  
Sudin Kumar Pandey ◽  
Twahira Begum ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Cinnamomum Zeylanicum ◽  
North East ◽  
Leaf Essential Oil ◽  
Anti Inflammatory

Background: Cinnamomum zeylanicum Blume bark and leaf essential oil possesses eugenol as the major component. Both the essential oil have the potential antioxidant, anti-inflammatory, antimicrobial and negligible genotoxicity. Objective: A comparative chemical composition and biological evaluation of Cinnamomum zeylanicum Blume, bark and leaf essential oils, originated from North-East India has been investigated in the present study. Methods: Gas chromatography/mass spectroscopy analysis was used to investigate the chemical composition of the essential oil samples. DPPH free radical scavenging activity, reducing power assay was used for antioxidant activity evaluation. Similarly, egg albumin denaturation assay, protease inhibitory activities were used for anti-inflammatory activity, while genotoxicity was evaluated by using Allium cepa assay. Results: The results showed that eugenol is the major component of C. zeylanicum bark (84.48%) and leaf (48.78 %) essential oil. Significant antioxidant activity were observed for both essential oils in the DPPH free radical scavenging assay (IC50= 103.2 μg/mL, bark; IC50= 234.7 μg/mL, leaf), and reducing power assay (absorbance of 1.802 nm, 0.907 nm in 48 µg/mL for bark and leaf essential oil respectively). In-vitro anti-inflammatory activity revealed strong potential of leaf essential oil (protein denaturation assay, IC50= 0.05279 μg/mL; protease inhibitory activity IC50= 3.607 μg/mL) in comparison to bark essential oil (protein denaturation assay IC50= 0.1491, protease inhibitory activity IC50= 61.06). Allium cepa root growth test, mitotic index, and chromosomal aberration test were performed to investigate the genotoxicity of C. zeylanicum bark and leaf essential oil, which resulted no toxic nature of both the essential oils. Antimicrobial activity against eight bacterial and fungal strains revealed good antimicrobial properties with the MIC value of Staphylococcus aureus at 5 μL/mL (bark), Aspergillus niger at 2.5 μL/mL (bark); Bacillus cereus at 5 μL/mL (leaf), Aspergillus niger at 2.5 μL/mL (leaf). Conclusion: Collectively, these findings indicated the strong biological potential of both essential oils, although bark essential possesses better biologically activities than the leaf essential oil and could be used in the food and beverages industries.

scholarly journals Pharamacognostic Profile and Comparative in vitro Anti- Inflammatory Activity Study of Ethanomedicinal Plants of North East India

2020 ◽  
Vol 13 (2) ◽  
pp. 317-324
Author(s):  
SM Abdul Aziz Barbhuiya ◽  
SH Victoria Devi ◽  
Ajay Kakati ◽  
Rejwan Ahmed Choudhury ◽  
Muslek Uddin Mazumder
Keyword(s):  
Inflammatory Activity ◽  
North East India ◽  
North East ◽  
Anti Inflammatory

scholarly journals In vitro and in vivo anti-inflammatory activity of Dillenia pentagyna Roxb. bark, a folklore medicine of Mizoram

2017 ◽  
Vol 17 (2) ◽  
pp. 69-77
Author(s):  
Zothanpuia ◽  
Bibhuti K. Kakoti
Keyword(s):  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Endangered Plants ◽  
Pharmacological Activities ◽  
North East India ◽  
Rich Variety ◽  
North East ◽  
Anti Inflammatory

Natural products have played an exceptionally important role in health care and prevention of diseases for thousands of years and are one of the greatest sources of medicine. Mizoram is a home to a rich variety of flora and fauna however; very less systematic survey and documentation were carried out to date. The plant diversity of Mizoram is not adequately studied as compared to other states of north east India. Dillenia pentagyna Roxb., known as kaihzawl in Mizo and dog teak in English, is one of the endangered plants that is yet to be explore. The Mizo communities use the decoction of D. pentagyna bark to treat different stomach ailments, inflammation and diabetes. In this perspective, the methanolic extract of the bark of D. pentagyna was evaluated for its anti-inflammatory properties. The extract showed the presence of flavanoids, triterpenoids, steroids, phenolics, saponins, fixed oils and others exerting varied pharmacological activities. The methanolic extract of this plant was found to contain active constituents such as betulinic acid and β-sitosterol which are found to actively interact with the inflammatory mediators and suppressed the inflammation when evaluated for its in vitro and in vivo anti-inflammatory activity. The present study suggests that the methanolic extract of D. pentagyna bark showed significant anti-inflammatory activity. This study also support the folkloric used of the plant in diseases related to inflammatory condition.

scholarly journals A Protocol for Rapid Clonal Propagation and Microrhizome Production of Curcuma caesia Roxb. (Zingiberaceae): A Critically Endangerd Medicinal Plant of North East India

2020 ◽  
Author(s):  
Indrani Sarma ◽  
A. C. Deka ◽  
T. C. Sarma
Keyword(s):  
Medicinal Plant ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Cost Effective ◽  
Northeast India ◽  
Ms Medium ◽  
North East India ◽  
North East ◽  
Best Response

Background: Curcuma caesia Roxb. (Zingiberaceae) is a rare, critically endangered medicinal plant of Northeast India. The rhizome of the plant is famous for its significant medicinal properties. Methods: Various concentrations of plant growth regulators in Murashige and Skoog (MS) medium were tried in the study using rhizome bud as explant for development of an efficient cost effective protocol for in vitro mass multiplication and microrhizome induction of Curcuma caesia. Result: Shoot multiplication and plant generation was achieved from freshly sprouted shoots of Curcuma caesia on Murashige and Skoog’s medium supplemented with different phytohormones. The best response for shoot multiplication (23.80±0.51shoots/explant) was obtained within 8 weeks in MS medium supplemented with BAP (1.0 mgl-1) and 2,4-D (0.25 mgl-1). Microrhizomes were induced at the base of the in vitro derived shoots upon transferred to medium containing various combinations and concentrations of sucrose and BAP and grown under varying photoperiod. Half strength of MS basal medium containing BAP (1.0 mg/l) and 9% sucrose was found to be optimum for induction of large sized microrhizome within 45 days of incubation under 16 hrs of photoperiod.

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