Saccharification of sugarcane bagasse by magnetic carbon-based solid acid pretreatment and enzymatic hydrolysis

Abstract The efficient conversion of hemicellulose and cellulose in lignocellulosic biomass to reducing sugars remains as one major challenge for the development of biorefinery. In this work, corncob saccharification in the aqueous phase was realized efficiently via pretreatment by magnetic carbon-based solid acid (MMCSA) catalyst, combined with the subsequent in situ enzymatic hydrolysis (occurring in the same pretreatment system after MMCSA separation). Under the optimized pretreatment conditions (the ratio of corncob, catalyst and water is 1:1:20 (g:g:mL), 160°C for 20 min) and in situ enzymatic hydrolysis (cellulase loading of 20 FPU / g, 24 h), an xylose yield of 88.77% and an enzymatic digestibility of 91.24% were obtained, respectively. Compared with the traditional enzymatic process, the present in situ enzymatic system has advantages of reduced enzyme loading, water consumption, and improved saccharification efficiency. Thus, this study provides a more sustainable and effective method for the saccharification of hemicellulose and cellulose in the corncob to produce reducing sugars.

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Enhanced enzymatic hydrolysis of poplar cellulosic residue fractionated by a magnetic carbon-based solid-acid catalyst in the γ-valerolactone–water system

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.114397 ◽

2022 ◽

Vol 176 ◽

pp. 114397

Author(s):

Qiong Wang ◽

Yunyun Liu ◽

Yi Zhang ◽

Yang Chen ◽

Qifa Feng ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Solid Acid ◽

Solid Acid Catalyst ◽

Water System ◽

Acid Catalyst ◽

Magnetic Carbon ◽

Hydrolysis Of ◽

Carbon Based

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Carbon-Based Solid Acid Pretreatment in Corncob Saccharification: Specific Xylose Production and Efficient Enzymatic Hydrolysis

ACS Sustainable Chemistry & Engineering ◽

10.1021/acssuschemeng.7b03959 ◽

2018 ◽

Vol 6 (3) ◽

pp. 3640-3648 ◽

Cited By ~ 28

Author(s):

Wei Qi ◽

Chao He ◽

Qiong Wang ◽

Shuna Liu ◽

Qiang Yu ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Solid Acid ◽

Acid Pretreatment ◽

Xylose Production ◽

Carbon Based

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A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

Biotechnology for Biofuels ◽

10.1186/s13068-021-01884-3 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Lin Dai ◽

Tian Huang ◽

Kankan Jiang ◽

Xin Zhou ◽

Yong Xu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Production Cost ◽

Solid Loading ◽

Fed Batch ◽

Acid Pretreatment ◽

Furoic Acid ◽

Cooling Crystallization ◽

Mineral Acids ◽

Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

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A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

10.21203/rs.3.rs-109722/v1 ◽

2020 ◽

Author(s):

Lin Dai ◽

Tian Huang ◽

Kankan Jiang ◽

Xin Zhou ◽

Yong Xu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Production Cost ◽

Solid Loading ◽

Fed Batch ◽

Acid Pretreatment ◽

Furoic Acid ◽

Cooling Crystallization ◽

Mineral Acids ◽

Hydrolysis Of

Abstract Background: Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results: The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 oC for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization.Conclusion: This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

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Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

Journal of Energy ◽

10.1155/2016/8506214 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 20

Author(s):

Robinson Timung ◽

Narendra Naik Deshavath ◽

Vaibhav V. Goud ◽

Venkata V. Dasu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Crystallinity Index ◽

Sem Analysis ◽

Reducing Sugar ◽

Dilute Acid Pretreatment ◽

Crystalline Cellulose ◽

Dilute Acid ◽

Acid Pretreatment ◽

Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

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