Evaluating requirements modeling methods based on user perceptions: A family of experiments

Intramembrane particles (IMP or MAP) are components of most biomembranes. They are visualized by freeze-fracture electron microscopy, and they probably represent replicas of integral membrane proteins. The presence of MAP in biomembranes has been extensively investigated but their detailed ultrastructure has been largely ignored. In this study, we have attempted to lay groundwork for a systematic evaluation of MAP ultrastructure. Using mathematical modeling methods, we have simulated the electron optical appearances of idealized globular proteins as they might be expected to appear in replicas under defined conditions. By comparing these images with the apearances of MAPs in replicas, we have attempted to evaluate dimensional and shape distortions that may be introduced by the freeze-fracture technique and further to deduce the actual shapes of integral membrane proteins from their freezefracture images.

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A Qualitative Examination of User Perceptions of User-Driven and App-Controlled Hearing Technologies

American Journal of Audiology ◽

10.1044/2019_aja-19-0022 ◽

2019 ◽

Vol 28 (4) ◽

pp. 993-1005 ◽

Cited By ~ 2

Author(s):

Gitte Keidser ◽

Nicole Matthews ◽

Elizabeth Convery

Keyword(s):

Hearing Aids ◽

Care Delivery ◽

Hearing Aid ◽

New Technology ◽

Successful Implementation ◽

Semistructured Interview ◽

User Perceptions ◽

Template Analysis ◽

Training And Support ◽

And Training

Purpose The aim of this study was to examine how hearing aid candidates perceive user-driven and app-controlled hearing aids and the effect these concepts have on traditional hearing health care delivery. Method Eleven adults (3 women, 8 men), recruited among 60 participants who had completed a research study evaluating an app-controlled, self-fitting hearing aid for 12 weeks, participated in a semistructured interview. Participants were over 55 years of age and had varied experience with hearing aids and smartphones. A template analysis was applied to data. Results Five themes emerged from the interviews: (a) prerequisites to the successful implementation of user-driven and app-controlled technologies, (b) benefits and advantages of user-driven and app-controlled technologies, (c) barriers to the acceptance and use of user-driven and app-controlled technologies, (d) beliefs that age is a significant factor in how well people will adopt new technology, and (e) consequences that flow from the adoption of user-driven and app-controlled technologies. Specifically, suggested benefits of the technology included fostering empowerment and providing cheaper and more discrete options, while challenges included lack of technological self-efficacy among older adults. Training and support were emphasized as necessary for successful adaptation and were suggested to be a focus of audiologic services in the future. Conclusion User perceptions of user-driven and app-controlled hearing technologies challenge the audiologic profession to provide adequate support and training for use of the technology and manufacturers to make the technology more accessible to older people.

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Facilitating at-home colorectal screening: the effect of cognitive and motivational instruction features on user perceptions and intentions

PsycEXTRA Dataset ◽

10.1037/e577642012-032 ◽

2006 ◽

Author(s):

Markus A. Feufel ◽

Tamera R. Schneider ◽

Hans J. Berkel

Keyword(s):

User Perceptions ◽

Colorectal Screening ◽

At Home

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Analysis of the operation of a two-phase ejector-disperser using numerical modeling methods

Automation Telemechanization and Communication in Oil Industry ◽

10.33285/0132-2222-2020-10(567)-5-8 ◽

2020 ◽

pp. 5-8

Author(s):

O.K. Shabalina ◽

Keyword(s):

Numerical Modeling ◽

Two Phase ◽

Modeling Methods

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Evaluation of the functional tension of foreign medical student’s organisms in adapting to educational activities

Russian Journal of Occupational Health and Industrial Ecology ◽

10.31089/1026-9428-2019-59-9-755-756 ◽

2020 ◽

pp. 755-755

Author(s):

N. K. Smagulov ◽

A. A. Adilbekova ◽

N. V. Gitenis

Keyword(s):

Mathematical Modeling ◽

Foreign Students ◽

Educational Process ◽

Educational Activities ◽

Modeling Methods ◽

Functional Stress

The use of mathematical modeling methods allowed us to develop criteria for functional stress for assessing and predicting the level of functional stress of foreign students in the process of adaptation to the educational process.

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FEATURES OF ORGANOPHOSPHATES IMMOBILIZATION VIA STREPTAVIDIN-BIOTIN SYSTEM FOR EXPERIMENTS ON SELECTION OF APTAMERS

Токсикологический вестник ◽

10.36946/0869-7922-2020-4-12-20 ◽

2020 ◽

pp. 12-20

Author(s):

D. A. Belinskaya ◽

Yu. V. Chelusnova ◽

V. V. Abzianidze ◽

N. V. Goncharov

Keyword(s):

Polyethylene Glycol ◽

Organophosphorus Compounds ◽

Binding Energies ◽

Rna Aptamers ◽

Main Method ◽

Modeling Methods ◽

Molecular Dynamics Methods ◽

Systematic Evolution ◽

Exponential Enrichment ◽

Selection Of

Poisoning with organophosphorus compounds occupy one of the leading places in exotoxicosis. At the first stage, the detoxification of organophosphates can be provided with the help of DNA or RNA aptamers that bind the poison in the bloodstream. Currently, the main method of searching for aptamers is the experimental method of systematic evolution of ligands by exponential enrichment (SELEX). In the process of aptamer selection, the target molecule must be immobilized via the streptavidin-biotin complex. Since the poison molecule is small in size, to increase its availability for binding to aptamer, it is necessary to use a spacer between organophosphorus compounds and biotin. The aim of this work was to optimize the selection of aptamers for organophosphorus compounds by increasing the availability of a poison molecule immobilized via the streptavidin-biotin complex on the example of paraoxon. For this purpose, three spacers between organophosphorus compounds and biotin were tested using molecular modeling methods: three links of polyethylene glycol (3-PEG), four links of polyethylene glycol (4-PEG) and aminohexyl. The conformation of the biotinylated paraoxon complex with streptavidin and the interaction of paraoxon with the binding fragment of the aptamer were modeled using molecular docking and molecular dynamics methods. The ability of biotinylated paraoxon to bind to the aptamer has been evaluated by analyzing the surface area of the paraoxon available to the solvent, as well as by calculating the free binding energies. It has been shown that only in the case of aminohexyl immobilized paraoxon can contact the aptamer. At the final stage, the synthesis of paraoxon bound to biotin via aminohexyl was carried out.

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