Administration of geniposide ameliorates dextran sulfate sodium-induced colitis in mice via inhibition of inflammation and mucosal damage

2017 ◽

Vol 2 (4) ◽

pp. 582-586

Author(s):

Md Shafiqul Islam ◽

Masatoshi Hori ◽

Hiroshi Ozaki

Keyword(s):

Extracellular Matrix ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Tissue Injury ◽

Goblet Cells ◽

Mucosal Damage ◽

Postnatal Life ◽

Tenascin C ◽

Moderate Staining ◽

High Level

Tenascin-C, an extracellular matrix glycoprotein, expresses high level during embryogenesis and almost absent during the normal postnatal life. However, it is re-appeared in a diverse condition such as tissue injury and in the stroma of various carcinomas. In this study, we investigated the appearance of tenascin-C in dextran sulfate sodium (DSS)-induced colitis in mice. DSS induced colitis mice demonstrated severe mucosal damage, with distortion and loss of crypts, depletion of goblet cells and infiltration of macrophages particularly F4/80 positive macrophages, granulocytes and lymphocytes in the colon tissues. These DSS inflamed colon expressed a high and dense level of tenascin-C in the severe damaged areas, whereas, moderate staining was observed in the moderate inflamed areas. DSS-induced colitis mice significantly increased macrophages infiltration in the colon tissues. These results suggested that tenascin-C extracellular matrix re-appeared in the colon tissues during inflammation.Asian J. Med. Biol. Res. December 2016, 2(4): 582-586

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Polyphenol Extract of Moringa Oleifera Leaves Alleviates Colonic Inflammation in Dextran Sulfate Sodium-Treated Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6295402 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Yunjuan Zhang ◽

Lei Peng ◽

Wenyun Li ◽

Tianyi Dai ◽

Long Nie ◽

...

Keyword(s):

Moringa Oleifera ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Activity Index ◽

Disease Activity Index ◽

Experimental Colitis ◽

Mucosal Damage ◽

Tnf Α ◽

Moringa Oleifera Leaves ◽

Moringa Oleifera Lam

Moringa oleifera Lam. is an essential herb used for the treatment of inflammation, diabetes, high blood pressure, and other diseases. In this study, phenolic extracts of M. oleifera leaves were obtained and analyzed. The results showed that the main identifiable phenols were astragalin, chlorogenic acid, isoquercitrin, kaempferitrin, luteolin, quercetin, and rutin. The effects of M. oleifera polyphenol extract (MOPE) on experimental colitis induced by 3% dextran sulfate sodium (DSS) were investigated. The results showed that oral administration of MOPE significantly alleviated the symptoms of DSS-induced colitis. MOPE significantly reduced weight loss, the disease activity index, colon shortening, and mucosal damage. In addition, MOPE attenuated the infiltration of CD3+ T cells, CD177+ neutrophils, and F4/80+ macrophages and significantly inhibited the secretion of IL-6 and TNF-α. After the MOPE administration, the expression of proteins associated with the NF-κB signaling pathway changed. Specifically, compared with that of the DSS group, the protein expression of NF-κB p65 and p-IκBα was downregulated, and the expression of IκBα was upregulated. This study revealed the anti-inflammatory effects and mechanisms of MOPE in the colon, indicating its potential use in preventing inflammation-driven diseases.

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