Association between high-density lipoprotein subfractions and low-grade inflammation, insulin resistance, and metabolic syndrome components: The ELSA-Brasil study

2018 ◽  
Vol 12 (5) ◽  
pp. 1290-1297.e1 ◽  
Author(s):  
Giuliano Generoso ◽  
Isabela M. Bensenor ◽  
Raul D. Santos ◽  
Itamar S. Santos ◽  
Alessandra C. Goulart ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Metabolic Syndrome ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Low Grade ◽  
Lipoprotein Subfractions ◽  
High Density Lipoprotein Subfractions ◽  
Low Grade Inflammation ◽  
Metabolic Syndrome Components

scholarly journals Adiponectin in Childhood and Adolescent Obesity and Its Association with Inflammatory Markers and Components of the Metabolic Syndrome

2006 ◽  
Vol 91 (11) ◽  
pp. 4415-4423 ◽  
Author(s):  
Jeffrey C. Winer ◽  
Tosca L. Zern ◽  
Sara E. Taksali ◽  
James Dziura ◽  
Anna M. G. Cali ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Metabolic Syndrome ◽  
Childhood Obesity ◽  
High Density Lipoprotein ◽  
Children And Adolescents ◽  
Density Lipoprotein ◽  
High Density ◽  
Low Grade ◽  
Obese Children ◽  
The Metabolic Syndrome

Abstract Context: Adiponectin levels are lower in obese children and adolescents, whereas markers of inflammation and proinflammatory cytokines are higher. Hypoadiponectinemia may contribute to the low-grade systemic chronic inflammatory state associated with childhood obesity. Objective: We investigated whether C-reactive protein (CRP), the prototype of inflammation, is related to adiponectin levels independently of insulin resistance and adiposity. Design, Setting, Participants, and Main Outcome Measures: In a multiethnic cohort of 589 obese children and adolescents, we administered a standard oral glucose tolerance test and obtained baseline measurements for adiponectin, plasma lipid profile, CRP, IL-6, and leptin. Results: Stratifying the cohort into quartiles of adiponectin levels and adjusting for potential confounding variables, such as age, gender, ethnicity, body mass index z-score, pubertal status, and insulin sensitivity, the present study revealed that low levels of adiponectin are associated not only with higher CRP levels, but also with components of the metabolic syndrome, such as low high-density lipoprotein cholesterol and a high triglyceride-to-high-density-lipoprotein ratio. Conclusions: The link between adiponectin levels and a strong marker of inflammation, CRP, is independent of insulin resistance and adiposity in obese children and adolescents. Adiponectin may be one of the signals linking inflammation and obesity. Thus, adiponectin may function as a biomarker of the metabolic syndrome in childhood obesity.

Abstract P195: High-density Lipoprotein Subfractions Are Associated With Low-grade Inflammation, Insulin Resistance and Metabolic Syndrome Components: The Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)

Circulation ◽  
2018 ◽  
Vol 137 (suppl_1) ◽  
Author(s):  
Giuliano Generoso ◽  
Isabela J Bensenor ◽  
Raul D Santos ◽  
Itamar S Santos ◽  
Alessandra C Goulart ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Cardiovascular Disease ◽  
Metabolic Syndrome ◽  
Univariate Analysis ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Low Grade ◽  
Adult Health ◽  
Lipoprotein Subfractions ◽  
Low Grade Inflammation

Background: HDL cholesterol (HDL-C) can be divided into subfractions, which may have variable association with atherogenic process. There are mixed results about the association between HDL-C subfractions and risk factors for cardiovascular disease. Hypothesis: HDL-C subfractions have different associations with metabolic syndrome, low-grade inflammation and insulin resistance. Methods: 4,532 individuals between 35 and 74 years without previous cardiovascular disease not in use of fibrates were enrolled. HDL-C subfractions were separated by vertical ultracentrifugation (Vertical Auto Profile - in mg/dl) in HDL 2 -C and HDL 3 -C. HDL 2 -C/HDL 3 -C ratio, insulin resistance (HOMA-IR) and high-sensitivity C-reactive protein (CRP) were also included in the analysis. Results: The mean age was 51 ± 9 years, and 54.8% were women. In univariate analysis, HDL-C, HDL 2 -C, and HDL 3 -C were all inversely associated with each of the MetS defining factors, HOMA-IR values, and serum CRP. It was also observed a negative association between HDL 2 -C/HDL 3 -C ratio with the variables mentioned above even after adjusting for smoking, alcohol use, physical activity, and HDL-C levels (p <0.01). Conclusion: HDL-C and both subfractions are inversely associated with all the factors that define MetS, insulin resistance and low-grade inflammation. Additionally, the HDL 2 -C/HDL 3 -C subfractions ratio assessed by VAP is also significantly associated with the former factors even after further adjustment for total HDL-C and other confounding variables.

High-density lipoprotein subfractions measured in stored serum

1994 ◽  
Vol 40 (9) ◽  
pp. 1713-1716 ◽  
Author(s):  
L L Bausserman ◽  
A L Saritelli ◽  
D Milosavljevic
Keyword(s):  
High Density Lipoprotein ◽  
Dextran Sulfate ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Manganese Chloride ◽  
Lipoprotein Subfractions ◽  
Hdl Subfractions ◽  
High Density Lipoprotein Subfractions

Abstract We compared the effects of freezing serum on the determination of high-density lipoprotein (HDL) subfractions by two dual-precipitation methods, heparin and manganese chloride/dextran sulfate (HM/DS) (Gidez et al., J Lipid Res 1982;23:1206-23) and DS/DS (Warnick et al., Clin Chem 1982;28:1574), and by ultracentrifugation. Storing serum for 1 month at -70 degrees C resulted in reduced HDL3-cholesterol by ultracentrifugation and reduced total and HDL3-cholesterol by the DS/DS method. There was no change in either total HDL-cholesterol or HDL3-cholesterol with the HM/DS method. Additional studies involving only HM/DS indicated that total HDL-cholesterol in serum stored at 4 degrees C begins to decline after 3 days (-3.1 +/- 3.5%, P &lt; 0.1). HDL was stable at -20 degrees C for 2 weeks but both total and HDL3-cholesterol decreased significantly after 1 month. Storage of serum at -70 degrees C resulted in no changes for 1 year; however, at 18 months, HDL3-cholesterol was reduced 13% (P = 0.002). We conclude that HDL subfractions can be determined accurately in serum as well as in plasma after storage at -70 degrees C for up to 1 year.

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