scholarly journals 3D microstructure reconstruction of casting aluminum alloy based on serial block-face scanning electron microscopy

2019 ◽  
Vol 778 ◽  
pp. 721-730 ◽  
Author(s):  
Jiawei Lu ◽  
Bo Chen ◽  
Xianping Liu ◽  
Fei Yang ◽  
Ian K. Robinson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Aluminum Alloy ◽  
3D Microstructure ◽  
Face Scanning ◽  
Microstructure Reconstruction ◽  
Casting Aluminum Alloy ◽  
Block Face ◽  
Casting Aluminum ◽  
Scanning Electron

scholarly journals Helical fibrillar microstructure of tendon using serial block-face scanning electron microscopy and a mechanical model for interfibrillar load transfer

2019 ◽  
Vol 16 (160) ◽  
pp. 20190547 ◽  
Author(s):  
Babak N. Safa ◽  
John M. Peloquin ◽  
Jessica R. Natriello ◽  
Jeffrey L. Caplan ◽  
Dawn M. Elliott
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Load Transfer ◽  
Three Dimensional ◽  
Helical Structures ◽  
3D Microstructure ◽  
Face Scanning ◽  
Block Face ◽  
Matrix Shear ◽  
Scanning Electron

Tendon's hierarchical structure allows for load transfer between its fibrillar elements at multiple length scales. Tendon microstructure is particularly important, because it includes the cells and their surrounding collagen fibrils, where mechanical interactions can have potentially important physiological and pathological contributions. However, the three-dimensional (3D) microstructure and the mechanisms of load transfer in that length scale are not known. It has been postulated that interfibrillar matrix shear or direct load transfer via the fusion/branching of small fibrils are responsible for load transfer, but the significance of these mechanisms is still unclear. Alternatively, the helical fibrils that occur at the microstructural scale in tendon may also mediate load transfer; however, these structures are not well studied due to the lack of a three-dimensional visualization of tendon microstructure. In this study, we used serial block-face scanning electron microscopy to investigate the 3D microstructure of fibrils in rat tail tendon. We found that tendon fibrils have a complex architecture with many helically wrapped fibrils. We studied the mechanical implications of these helical structures using finite-element modelling and found that frictional contact between helical fibrils can induce load transfer even in the absence of matrix bonding or fibril fusion/branching. This study is significant in that it provides a three-dimensional view of the tendon microstructure and suggests friction between helically wrapped fibrils as a mechanism for load transfer, which is an important aspect of tendon biomechanics.

scholarly journals Compact-sized Cutting System for a Serial-block-face Scanning Electron Microscopy

2021 ◽  
Vol 27 (S1) ◽  
pp. 3176-3177
Author(s):  
Nanami Takagi ◽  
Norio Yamashita ◽  
Yuki Tsujimura ◽  
Hiroshi Takemura ◽  
Sze Keat Chee ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Face Scanning ◽  
Block Face ◽  
Cutting System ◽  
Scanning Electron

scholarly journals Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis

2017 ◽  
Vol 130 (10) ◽  
pp. 1845-1855 ◽  
Author(s):  
Faye M. Nixon ◽  
Thomas R. Honnor ◽  
Nicholas I. Clarke ◽  
Georgina P. Starling ◽  
Alison J. Beckett ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Mitotic Spindles ◽  
Microtubule Organization ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

10.3791/62045 ◽  
2021 ◽  
Author(s):  
Justin A. Courson ◽  
Paul T. Landry ◽  
Thao Do ◽  
Eric Spehlmann ◽  
Pascal J. Lafontant ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Biological Tissue ◽  
Tissue Samples ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

scholarly journals Staining and Embedding of Human Chromosomes for 3-D Serial Block-Face Scanning Electron Microscopy

BioTechniques ◽  
2014 ◽  
Vol 57 (6) ◽  
Author(s):  
Mohammed Yusuf ◽  
Bo Chen ◽  
Teruo Hashimoto ◽  
Ana Katrina Estandarte ◽  
George Thompson ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Chromosomes ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron
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