Pellets were collected between 1995 and 2016, from 53 localities (investigated area: XL89, XL99, YL08, YL09, XM52, XM61, XM62, XM70, XM72, XM74, XM80, XM81, XM82, XM83, XM84, XM90, XM91, XM93, XM95, YM02, YM03, YM12, YM13, YM14, YM22, YM23, YM24 and BS73, according to 10×10 km UTM grids). In a total of 5164 Barn Owl pellets 14360 prey remnants were found. In the diet of Barn Owls small mammals dominated (98.6%). From the prey items the presence of 27 mammal species (Croci-dura leucodon, C. suaveolens, Sorex araneus, S. minutus, Neomys anomalus, N. fodiens, Talpa europaea, Eptesi-cus serotinus, Nyctalus leisleri, N. noctula, Myotis myotis, Muscardinus avellanarius, Microtus agrestis, M. arvalis, M. subterraneus, Arvicola amphibius, Myodes glareolus, Apo-demus agrarius, A. flavicollis, A. sylvaticus, A. uralensis, Micromys minutus, Mus musculus, M. spicilegus, Rattus norvegicus, R. rattus, Mustela nivalis) was confirmed. Rem-nants of birds, amphibians and insects made up only 1.4% of total prey.