scholarly journals Valveless pumping mechanics of the embryonic heart during cardiac looping: Pressure and flow through micro-PIV

2017 ◽  
Vol 50 ◽  
pp. 50-55 ◽  
Author(s):  
D.L. Bark ◽  
B. Johnson ◽  
D. Garrity ◽  
L.P. Dasi
Keyword(s):  
Embryonic Heart ◽  
Cardiac Looping ◽  
Micro Piv ◽  
Valveless Pumping ◽  
Flow Through

scholarly journals Blood flow through the embryonic heart outflow tract during cardiac looping in HH13–HH18 chicken embryos

2015 ◽  
Vol 12 (111) ◽  
pp. 20150652 ◽  
Author(s):  
Madeline Midgett ◽  
Venkat Keshav Chivukula ◽  
Calder Dorn ◽  
Samantha Wallace ◽  
Sandra Rugonyi
Keyword(s):  
Blood Flow ◽  
Cardiac Development ◽  
Structural Information ◽  
Blood Flow Rate ◽  
Embryonic Heart ◽  
Outflow Tract ◽  
Cardiac Looping ◽  
Chicken Embryos ◽  
Normal Blood Flow ◽  
Flow Through

Blood flow is inherently linked to embryonic cardiac development, as haemodynamic forces exerted by flow stimulate mechanotransduction mechanisms that modulate cardiac growth and remodelling. This study evaluated blood flow in the embryonic heart outflow tract (OFT) during normal development at each stage between HH13 and HH18 in chicken embryos, in order to characterize changes in haemodynamic conditions during critical cardiac looping transformations. Two-dimensional optical coherence tomography was used to simultaneously acquire both structural and Doppler flow images, in order to extract blood flow velocity and structural information and estimate haemodynamic measures. From HH13 to HH18, peak blood flow rate increased by 2.4-fold and stroke volume increased by 2.1-fold. Wall shear rate (WSR) and lumen diameter data suggest that changes in blood flow during HH13–HH18 may induce a shear-mediated vasodilation response in the OFT. Embryo-specific four-dimensional computational fluid dynamics modelling at HH13 and HH18 complemented experimental observations and indicated heterogeneous WSR distributions over the OFT. Characterizing changes in haemodynamics during cardiac looping will help us better understand the way normal blood flow impacts proper cardiac development.

scholarly journals Functional Morphology of the Cardiac Jelly in the Tubular Heart of Vertebrate Embryos

2019 ◽  
Author(s):  
Jörg Männer ◽  
Talat Mesud Yelbuz
Keyword(s):  
Spatial Distribution ◽  
Cross Sections ◽  
Embryonic Heart ◽  
Endocardial Cushions ◽  
Valveless Pumping ◽  
Matrix Layer ◽  
Heart Looping ◽  
Biological Aspects ◽  
Vertebrate Embryos ◽  
Heart Wall

The early embryonic heart is a multi-layered tube consisting of (1) an outer myocardial tube; (2) an inner endocardial tube; and (3) an extracellular matrix layer interposed between myocardium and endocardium, called “cardiac jelly” (CJ). During the past decades, research on CJ has mainly focused on its molecular and cell biological aspects. This review focuses on the morphological and biomechanical aspects of CJ. Special attention is given to (1) the spatial distribution and fiber architecture of CJ; (2) the morphological dynamics of CJ during the cardiac cycle; and (3) the removal/remodeling of CJ during advanced heart looping stages, which leads to the formation of ventricular trabeculations and endocardial cushions. CJ acts as a hydraulic skeleton displaying striking structural and functional similarities with the mesoglea of jellyfish. CJ not only represents a filler substance, facilitating end-systolic occlusion of the embryonic heart lumen. Its elastic components antagonize the systolic deformations of the heart wall and thereby power the refilling phase of the ventricular tube. Non-uniform spatial distribution of CJ generates non-circular cross sections of the opened endocardial tube (initially elliptic, later deltoid), which seem to be advantageous for valveless pumping. Endocardial cushions arise from non-removed remnants of the original CJ.

scholarly journals The Complexity of Porous Media Flow Characterized in a Microfluidic Model Based on Confocal Laser Scanning Microscopy and Micro-PIV

2020 ◽  
Author(s):  
D. A. M. de Winter ◽  
K. Weishaupt ◽  
S. Scheller ◽  
S. Frey ◽  
A. Raoof ◽  
...  
Keyword(s):  
Porous Media ◽  
Fluid Flow ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Pore Scale ◽  
Micro Piv ◽  
Flow Through

Abstract In this study, the complexity of a steady-state flow through porous media is revealed using confocal laser scanning microscopy (CLSM). Micro-particle image velocimetry (micro-PIV) is applied to construct movies of colloidal particles. The calculated velocity vector fields from images are further utilized to obtain laminar flow streamlines. Fluid flow through a single straight channel is used to confirm that quantitative CLSM measurements can be conducted. Next, the coupling between the flow in a channel and the movement within an intersecting dead-end region is studied. Quantitative CLSM measurements confirm the numerically determined coupling parameter from earlier work of the authors. The fluid flow complexity is demonstrated using a porous medium consisting of a regular grid of pores in contact with a flowing fluid channel. The porous media structure was further used as the simulation domain for numerical modeling. Both the simulation, based on solving Stokes equations, and the experimental data show presence of non-trivial streamline trajectories across the pore structures. In view of the results, we argue that the hydrodynamic mixing is a combination of non-trivial streamline routing and Brownian motion by pore-scale diffusion. The results provide insight into challenges in upscaling hydrodynamic dispersion from pore scale to representative elementary volume (REV) scale. Furthermore, the successful quantitative validation of CLSM-based data from a microfluidic model fed by an electrical syringe pump provided a valuable benchmark for qualitative validation of computer simulation results. Graphic Abstract

215 Measurement of blood flow through bifurcation and confluence of microchannel with confocal micro-PIV system

2009 ◽  
Vol 2008.21 (0) ◽  
pp. 87-88
Author(s):  
Hiroki FUJIWARA ◽  
Takuji ISHIKAWA ◽  
Noriaki MATSUKI ◽  
Takefumi YOSHIMOTO ◽  
Yohsuke IMAI ◽  
...  
Keyword(s):  
Blood Flow ◽  
Micro Piv ◽  
Flow Through

scholarly journals Functional Morphology of the Cardiac Jelly in the Tubular Heart of Vertebrate Embryos

2019 ◽  
Vol 6 (1) ◽  
pp. 12 ◽  
Author(s):  
Jörg Männer ◽  
Talat Mesud Männer
Keyword(s):  
Spatial Distribution ◽  
Cross Sections ◽  
Embryonic Heart ◽  
Endocardial Cushions ◽  
Valveless Pumping ◽  
Matrix Layer ◽  
Heart Looping ◽  
Biological Aspects ◽  
Vertebrate Embryos ◽  
Heart Wall

The early embryonic heart is a multi-layered tube consisting of (1) an outer myocardial tube; (2) an inner endocardial tube; and (3) an extracellular matrix layer interposed between the myocardium and endocardium, called “cardiac jelly” (CJ). During the past decades, research on CJ has mainly focused on its molecular and cellular biological aspects. This review focuses on the morphological and biomechanical aspects of CJ. Special attention is given to (1) the spatial distribution and fiber architecture of CJ; (2) the morphological dynamics of CJ during the cardiac cycle; and (3) the removal/remodeling of CJ during advanced heart looping stages, which leads to the formation of ventricular trabeculations and endocardial cushions. CJ acts as a hydraulic skeleton, displaying striking structural and functional similarities with the mesoglea of jellyfish. CJ not only represents a filler substance, facilitating end-systolic occlusion of the embryonic heart lumen. Its elastic components antagonize the systolic deformations of the heart wall and thereby power the refilling phase of the ventricular tube. Non-uniform spatial distribution of CJ generates non-circular cross sections of the opened endocardial tube (initially elliptic, later deltoid), which seem to be advantageous for valveless pumping. Endocardial cushions/ridges are cellularized remnants of non-removed CJ.

Development of apical pits in chloride cells of the gills of Pimephales promelas after chronic exposure to acid water

1983 ◽  
Vol 41 ◽  
pp. 462-463
Author(s):  
Richard L. Leino ◽  
Jon G. Anderson ◽  
J. Howard McCormick
Keyword(s):  
Confidence Interval ◽  
Chronic Exposure ◽  
Lake Superior ◽  
Pimephales Promelas ◽  
Chloride Cells ◽  
Fathead Minnows ◽  
Secondary Lamellae ◽  
Untreated Water ◽  
Water Ph ◽  
Flow Through

Groups of 12 fathead minnows were exposed for 129 days to Lake Superior water acidified (pH 5.0, 5.5, 6.0 or 6.5) with reagent grade H2SO4 by means of a multichannel toxicant system for flow-through bioassays. Untreated water (pH 7.5) had the following properties: hardness 45.3 ± 0.3 (95% confidence interval) mg/1 as CaCO3; alkalinity 42.6 ± 0.2 mg/1; Cl- 0.03 meq/1; Na+ 0.05 meq/1; K+ 0.01 meq/1; Ca2+ 0.68 meq/1; Mg2+ 0.26 meq/1; dissolved O2 5.8 ± 0.3 mg/1; free CO2 3.2 ± 0.4 mg/1; T= 24.3 ± 0.1°C. The 1st, 2nd and 3rd gills were subsequently processed for LM (methacrylate), TEM and SEM respectively.Three changes involving chloride cells were correlated with increasing acidity: 1) the appearance of apical pits (figs. 2,5 as compared to figs. 1, 3,4) in chloride cells (about 22% of the chloride cells had pits at pH 5.0); 2) increases in their numbers and 3) increases in the % of these cells in the epithelium of the secondary lamellae.

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