Analysis of iron- and sulfur-oxidizing bacteria in a treatment plant of acid rock drainage from a Japanese pyrite mine by use of ribulose-1, 5-bisphosphate carboxylase/oxygenase large-subunit gene

2010 ◽  
Vol 109 (3) ◽  
pp. 244-248 ◽  
Author(s):  
Kazuo Kamimura ◽  
Ai Okabayashi ◽  
Mei Kikumoto ◽  
Mohammed Abul Manchur ◽  
Satoshi Wakai ◽  
...  
Keyword(s):  
Acid Rock Drainage ◽  
Large Subunit ◽  
Treatment Plant ◽  
Subunit Gene ◽  
Acid Rock ◽  
Bisphosphate Carboxylase ◽  
Ribulose 1 ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

scholarly journals Removal of Hydrogen Sulfide in Septic Tanks for Treating Black Water via an Immobilized Media of Sulfur-Oxidizing Bacteria

2020 ◽  
Vol 17 (3) ◽  
pp. 684
Author(s):  
Jeong-Hee Kang ◽  
Hyeong-Gyu Namgung ◽  
Jeong-Il Cho ◽  
Sung Soo Yoo ◽  
Bong-Jae Lee ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Removal Efficiency ◽  
Treatment Plant ◽  
Aeration Rate ◽  
H2s Removal ◽  
Septic Tanks ◽  
Black Water ◽  
Cultivation Period ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

In South Korea, the installation of septic tanks for treating black water (STBW) is regulated even in sewage treatment areas to prevent the black water deposition in combined sewers. STBWs in which black water is anaerobically decomposed generate high concentrations of hydrogen sulfide (H2S). In this study, an immobilized media of sulfur-oxidizing bacteria (SOB) was used to remove the H2S. SOB media was prepared by using activated sludge collected from a wastewater treatment plant. Prior to field application, an appropriate cultivation period and aeration rate for SOB activation were estimated through a laboratory-scale test. The SOB was activated after a 23-day cultivation period and an aeration rate of 0.25 L-water/L-air/min. Moreover, the maximum H2S removal efficiency was observed at a cultivation period of 43 days and an aeration rate of 0.38 L-water/L-air/min. Then, the SOB media was installed on STBWs of various capacities. The H2S removal efficiency was compared between with and without SOB media. The maximum H2S elimination capacity with SOB media was 12.3 g/m3/h, which was approximately three times higher than without SOB media. Furthermore, the energy efficiency and oxidation rate were also three times higher with SOB, demonstrating the applicability of SOB for H2S removal in STBW.

scholarly journals In vitro synthesis and processing of a maize chloroplast transcript encoded by the ribulose 1,5-bisphosphate carboxylase large subunit gene.

1985 ◽  
Vol 5 (10) ◽  
pp. 2733-2745 ◽  
Author(s):  
L Hanley-Bowdoin ◽  
E M Orozco ◽  
N H Chua
Keyword(s):  
Transcription Initiation ◽  
Large Subunit ◽  
Plastid Dna ◽  
Subunit Gene ◽  
Coding Region ◽  
Protein Coding ◽  
Bisphosphate Carboxylase ◽  
Transcription In Vitro ◽  
Maize Chloroplast

The large subunit gene (rbcL) of ribulose 1,5-bisphosphate carboxylase was transcribed in vitro by using maize and pea chloroplast extracts and a cloned plastid DNA template containing 172 base pairs (bp) of the maize rbcL protein-coding region and 791 bp of upstream sequences. Three major in vitro RNA species were synthesized which correspond to in vivo maize rbcL RNAs with 5' termini positioned 300, 100 to 105, and 63 nucleotides upstream of the protein-coding region. A deletion of 109 bp, including the "-300" 5' end (the 5' end at position -300), depressed all rbcL transcription in vitro. A plasmid DNA containing this 109-bp fragment was sufficient to direct correct transcription initiation in vitro. A cloned template, containing 191 bp of plastid DNA which includes the -105 and -63 rbcL termini, did not support transcription in vitro. Exogenously added -300 RNA could be converted to the -63 transcript by maize chloroplast extract. These results established that the -300 RNA is the primary maize rbcL transcript, the -63 RNA is a processed form of the -300 transcript, and synthesis of the -105 RNA is dependent on the -300 region. The promoter for the maize rbcL gene is located within the 109 bp flanking the -300 site. Mutagenesis of the 109-bp chloroplast sequence 11 bp upstream of the -300 transcription initiation site reduced rbcL promoter activity in vitro.

scholarly journals Occurrence, phylogeny and evolution of ribulose-1,5-bisphosphate carboxylase/oxygenase genes in obligately chemolithoautotrophic sulfur-oxidizing bacteria of the genera Thiomicrospira and Thioalkalimicrobium

Microbiology ◽  
2006 ◽  
Vol 152 (7) ◽  
pp. 2159-2169 ◽  
Author(s):  
Tatjana P. Tourova ◽  
Elizaveta M. Spiridonova ◽  
Ivan A. Berg ◽  
Boris B. Kuznetsov ◽  
Dimitry Yu. Sorokin
Keyword(s):  
16S Rrna ◽  
Large Subunit ◽  
Gene Encoding ◽  
Bisphosphate Carboxylase ◽  
Phylogenetic Cluster ◽  
New Family ◽  
Genes Encoding ◽  
Key Enzyme ◽  
Sulfur Oxidizing ◽  
Phylogeny And Evolution

The occurrence of the different genes encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the key enzyme of the Calvin–Benson–Bassham cycle of autotrophic CO2 fixation, was investigated in the members of the genus Thiomicrospira and the relative genus Thioalkalimicrobium, all obligately chemolithoautotrophic sulfur-oxidizing Gammaproteobacteria. The cbbL gene encoding the ‘green-like’ form I RubisCO large subunit was found in all analysed species, while the cbbM gene encoding form II RubisCO was present only in Thiomicrospira species. Furthermore, species belonging to the Thiomicrospira crunogena 16S rRNA-based phylogenetic cluster also possessed two genes of green-like form I RubisCO, cbbL-1 and cbbL-2. Both 16S-rRNA- and cbbL-based phylogenies of the Thiomicrospira–Thioalkalimicrobium–Hydrogenovibrio group were congruent, thus supporting its monophyletic origin. On the other hand, it also supports the necessity for taxonomy reorganization of this group into a new family with four genera.

Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA

Cell ◽  
1983 ◽  
Vol 34 (3) ◽  
pp. 1007-1014 ◽  
Author(s):  
David M. Lonsdale ◽  
Tony P. Hodge ◽  
Christopher J. Howe ◽  
David B. Stem
Keyword(s):  
Mitochondrial Dna ◽  
Chloroplast Dna ◽  
Large Subunit ◽  
Subunit Gene ◽  
Bisphosphate Carboxylase

Enriching acid rock drainage related microbial communities from surface-deposited oil sands tailings

2016 ◽  
Vol 62 (10) ◽  
pp. 870-879 ◽  
Author(s):  
Courtney Dean ◽  
Yeyuan Xiao ◽  
Deborah J. Roberts
Keyword(s):  
Microbial Communities ◽  
Oil Sands ◽  
Acid Rock Drainage ◽  
Model Organisms ◽  
Acid Rock ◽  
Microbial Community Structures ◽  
Sulfur Oxidizing ◽  
Different Populations ◽  
Oil Sands Tailings ◽  
Halothiobacillus Neapolitanus

Little is known about the microbial communities native to surface-deposited pyritic oil sands tailings, an environment where acid rock drainage (ARD) could occur. The goal of this study was to enrich sulfur-oxidizing organisms from these tailings and determine whether different populations exist at pH levels 7, 4.5, and 2.5. Using growth-based methods provides model organisms for use in the future to predict potential activities and limitations of these organisms and to develop possible control methods. Thiosulfate-fed enrichment cultures were monitored for approximately 1 year. The results showed that the enrichments at pH 4.5 and 7 were established quicker than at pH 2.5. Different microbial community structures were found among the 3 pH environments. The sulfur-oxidizing microorganisms identified were most closely related to Halothiobacillus neapolitanus, Achromobacter spp., and Curtobacterium spp. While microorganisms related to Chitinophagaceae and Acidocella spp. were identified as the only possible iron-oxidizing and -reducing microbes. These results contribute to the general knowledge of the relatively understudied microbial communities that exist in pyritic oil sands tailings and indicate these communities may have a potential role in ARD generation, which may have implications for future tailings management.

Characteristics of Cultivated Sulfur Oxidizing Bacteria Community Isolated from Coal Mine Treatment Plant in H2S Removal

2016 ◽  
Vol 848 ◽  
pp. 127-130
Author(s):  
Duongruitai Nicomrat
Keyword(s):  
Coal Mine ◽  
Treatment Plant ◽  
Most Probable Number ◽  
Sulfur Cycling ◽  
Probable Number ◽  
Treatment Facilities ◽  
Sulfur Oxidizing ◽  
Mae Moh ◽  
Over Time ◽  
Sulfur Oxidizing Bacteria

Sulfur cycling based on biological oxidation of sulfide to sulfate involves sulfur-oxidizing reducing microbial communities associated with sulfide which normally oxidize sulfide in acidic environment to sulfate. The chemolithotrophic sulfur oxidizing bacteria (SOB) usually use organic and/ or inorganic sulfide initially oxidizing and subsequently released sulfate under aerobic or subaerobic condition. This study was to understand SOB community isolated from coal mine treating plant at Mae Moh, Lampang and their potentials in hydrogen sulfide (H2S) removal. In this result, with common heterotrophic medium supplemented with glucose 10 mg/ L and sulfur 0.001%, the cultured SOB were successfully cultivated based on most probable number method. Their activity showed an increase in sulfate concentration over time correspondent to an increase in culturable SOB communities. The SOB community could also develop their own microbial niches in the present of continuously aerated H2S in the medium at 30-40°C during 7 day incubation. They could remove H2S between 200-500 ppm from wastewater. These sulfur oxidized H2S contaminated in wastewater treatment facilities or drainage. Their species will be further isolated and characterized by molecular analysis.

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