Optimal production of bioflocculant from Pseudomonas sp. GO2 and its removal characteristics of heavy metals

Author(s):  
Jiayin Feng ◽  
Yijie Xu ◽  
Jianhui Ding ◽  
Jikun He ◽  
Yihan Shen ◽  
...  
2010 ◽  
Vol 29 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Hee-Jung Kim ◽  
Ri-Bi Yoo ◽  
Seok-Soon Han ◽  
Sun-Hee Woo ◽  
Moon-Soon Lee ◽  
...  

Chemosphere ◽  
2020 ◽  
Vol 240 ◽  
pp. 124893 ◽  
Author(s):  
Shaozu Xu ◽  
Yonghui Xing ◽  
Song Liu ◽  
Xiuli Hao ◽  
Wenli Chen ◽  
...  

2014 ◽  
Vol 68 ◽  
pp. 300-308 ◽  
Author(s):  
Liang Chen ◽  
Shenglian Luo ◽  
Xiaojie Li ◽  
Yong Wan ◽  
Jueliang Chen ◽  
...  

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Sudaryono Sudaryono

Absorption of heavy metals by plants is influenced by soil and plant biology. Heavy metals are absorbed into plant tissues through the root, then enter the food chain cycle. Metal will accumulate in body tissues and can have negative impacts for humans, animals, and plants when exceeding the limit of tolerance. Heavy metal accumulation in plant tissues can directly affect plant growth and production, can indirectly affect human health when consuming food contaminated heavy metals. The research objective was to determine the ability of biological fertilizers containing bacteria Azotobacter sp. and Pseudomonas sp., in absorbing heavy metals (Cu and Pb) contained in landfill waste compost, which is used to fertilize crops corn (baby corn). The research methodologyuses a split plot design arranged in Completely Randomized Design with 3 blocks as replicates. The first factor that serves as the main plot of the biological fertilizer, consisting of two cedars, namely: no biological fertilizers and biological fertilizers. The second factor that functions as a sub plot that is a combination of landfill waste compost with NPK fertilizer, consisting of four treatments, namely: Compost waste landfill: no fertilizer NPK = 1: 0; compost waste landfill: NPK = 2/3 : 1/3; compost waste landfill: fertilizer NPK = 1/3: 2/3Without landfill waste compost: NPK = 0: 1. The results showed that administration of biological fertilizers in combination with compost waste landfill is more dominant for increasing and improving soil physical chemistry, such as increasing soil pH, nutrients nitrogen, phosphorus and potassium and cation exchange capacity (KPK). Biological fertilizer containing bacteria Azotobacter sp. and Pseudomonas sp. may reduce the uptake of heavy metals Cu to below the threshold of 2.526 to 15.79 ppm, the maximum content of Cu ranges between 20-100 ppm. The mean content of Pb in tissues and cobs of corn is above the maximum limit of 29.80 to 42.69 ppm, which is still allowed Pb content between 0.1 to 10 ppm. Means baby corn crop is not safe for consumption.key words: compost from the landfill, heavy metals, biological fertilizer


2018 ◽  
Vol 111 ◽  
pp. 236-240
Author(s):  
Yangyang Feng ◽  
Xian Xu ◽  
Lei Zhang ◽  
Chenyang Zhang
Keyword(s):  

Author(s):  
Ahmed M. AZZAM ◽  
Ahmed TAWFIK

Bio-flocculants produced by Bacillus sp. and Pseudomonas sp. were evaluated as flocculating agents for the removal of Cu (II), Pb (II) and Cd (II) from chemical and textile wastewater industries. Both bio-flocculants were very effective for removal of heavy metals at a dosage not exceeding 0.1 mg/ml. However, the removal efficiency of heavy metals was dependant on initial concentration and type of bio-flocculants. 84.0% of Cu2+ and 99.5% of Pb2+ were removed from industrial wastewater using Bacillus sp. Bio-flocculant resulting residual values of 28.5 and 1.13μg/l respectively in the treated effluent. Lower removal efficiencies of 70.4% for Cu2+ and 97.8% for Pb2+ occurred using Pseudomonas sp. bioflocculant. Nevertheless, Pseudomonas sp. bio-flocculant achieved a substantially higher removal efficiency of Cd2+ (93.5%) as compared to 72.9% using Bacillus sp. Based on these results bio-flocculants are considered as a viable alternative for the treatment of industrial wastewater containing heavy metals.


2019 ◽  
Vol 5 (03) ◽  
pp. 170-185
Author(s):  
Rajesh Bajpai ◽  
C. P. Singh ◽  
D. K. Upreti

Soil microflora is continuously changing with altered soil conditions. These soil alterations are a consequence of heavy metals entering and affecting every sphere of life. Heavy metals are not only hazardous for crops but also affect the soil microbial community. Soil bacteria with the potential of plant growth promotion and multiple metal resistances can be an instrument for crop improvement and heavy metal detoxification. In this study, predominant bacterial community associated with the heavy metal contaminated soil was studied using 16S rRNA gene sequencing in association with culture-based techniques. Elemental metal analysis of collected soil samples showed an elevated level of metal content in the soil. 16S rRNA gene analysis and phylogenetic analysis of 126 bacterial clones revealed the probable predominance of Pseudomonas (40.48%) followed by Flavisolibacter (13.49%). Based on morphological and biochemical characterization, nine Pseudomonas strains were selected from the soil and were further confirmed by 16S rRNA gene sequencing with 92%-100% similarity with Pseudomonas species. The minimum inhibitory concentration (MIC) and maximum tolerance capacity (MTC) of three essential metals Cu, Zn, and Fe were determined individually and in combinations. It was found that Zn is the most toxic metal among the three metals and the metal showed a synergistic effect in inhibiting microbial growth when used in combinations. Presence of three metal resistant/tolerant genes czcA, pcoA and copB were also determined in the isolated Pseudomonas sp. by PCR. The soil in this region has high concentrations of heavy metals. The indigenous Pseudomonas sp. has multiple metal resistances and can be used for bioremediation of heavy metals and microbe assisted phytoremediation.


2019 ◽  
Vol 5 (01) ◽  
pp. 26-32 ◽  
Author(s):  
Sanjeev Kumar ◽  
Arindam Adhikary ◽  
Rashmi Saini ◽  
Pankaj Bhardwaj

Soil microflora is continuously changing with altered soil conditions. These soil alterations are a consequence of heavy metals entering and affecting every sphere of life. Heavy metals are not only hazardous for crops but also affect the soil microbial community. Soil bacteria with the potential of plant growth promotion and multiple metal resistances can be an instrument for crop improvement and heavy metal detoxification. In this study, predominant bacterial community associated with the heavy metal contaminated soil was studied using 16S rRNA gene sequencing in association with culture-based techniques. Elemental metal analysis of collected soil samples showed an elevated level of metal content in the soil. 16S rRNA gene analysis and phylogenetic analysis of 126 bacterial clones revealed the probable predominance of Pseudomonas (40.48%) followed by Flavisolibacter (13.49%). Based on morphological and biochemical characterization, nine Pseudomonas strains were selected from the soil and were further confirmed by 16S rRNA gene sequencing with 92%-100% similarity with Pseudomonas species. The minimum inhibitory concentration (MIC) and maximum tolerance capacity (MTC) of three essential metals Cu, Zn, and Fe were determined individually and in combinations. It was found that Zn is the most toxic metal among the three metals and the metal showed a synergistic effect in inhibiting microbial growth when used in combinations. Presence of three metal resistant/tolerant genes czcA, pcoA and copB were also determined in the isolated Pseudomonas sp. by PCR. The soil in this region has high concentrations of heavy metals. The indigenous Pseudomonas sp. has multiple metal resistances and can be used for bioremediation of heavy metals and microbe assisted phytoremediation.


2020 ◽  
Vol 77 (10) ◽  
pp. 2694-2701 ◽  
Author(s):  
Micaela Santana Ramos ◽  
João Pedro Rueda Furlan ◽  
Inara Fernanda Lage Gallo ◽  
Lucas David Rodrigues dos Santos ◽  
Tatiana Amabile de Campos ◽  
...  

2016 ◽  
Vol 2 (1 and 2) ◽  
pp. 43-58
Author(s):  
Neera Garg ◽  
Priyanka Singla

Soil microflora is continuously changing with altered soil conditions. These soil alterations are a consequence of heavy metals entering and affecting every sphere of life. Heavy metals are not only hazardous for crops but also affect the soil microbial community. Soil bacteria with the potential of plant growth promotion and multiple metal resistances can be an instrument for crop improvement and heavy metal detoxification. In this study, predominant bacterial community associated with the heavy metal contaminated soil was studied using 16S rRNA gene sequencing in association with culture-based techniques. Elemental metal analysis of collected soil samples showed an elevated level of metal content in the soil. 16S rRNA gene analysis and phylogenetic analysis of 126 bacterial clones revealed the probable predominance of Pseudomonas (40.48%) followed by Flavisolibacter (13.49%). Based on morphological and biochemical characterization, nine Pseudomonas strains were selected from the soil and were further confirmed by 16S rRNA gene sequencing with 92%-100% similarity with Pseudomonas species. The minimum inhibitory concentration (MIC) and maximum tolerance capacity (MTC) of three essential metals Cu, Zn, and Fe were determined individually and in combinations. It was found that Zn is the most toxic metal among the three metals and the metal showed a synergistic effect in inhibiting microbial growth when used in combinations. Presence of three metal resistant/tolerant genes czcA, pcoA and copB were also determined in the isolated Pseudomonas sp. by PCR. The soil in this region has high concentrations of heavy metals. The indigenous Pseudomonas sp. has multiple metal resistances and can be used for bioremediation of heavy metals and microbe assisted phytoremediation.


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