Stable-isotope dilution GC–MS approach for nitrite quantification in human whole blood, erythrocytes, and plasma using pentafluorobenzyl bromide derivatization: Nitrite distribution in human blood

2011 ◽  
Vol 879 (17-18) ◽  
pp. 1485-1495 ◽  
Author(s):  
Alexandra Schwarz ◽  
Darko Modun ◽  
Karsten Heusser ◽  
Jens Tank ◽  
Frank-Mathias Gutzki ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Human Blood ◽  
Isotope Dilution ◽  
Whole Blood ◽  
Human Whole Blood ◽  
Stable Isotope Dilution ◽  
Pentafluorobenzyl Bromide

Determination of lead in urine and whole blood by stable isotope dilution gas chromatography-mass spectrometry

1994 ◽  
Vol 40 (8) ◽  
pp. 1494-1502 ◽  
Author(s):  
S K Aggarwal ◽  
M Kinter ◽  
D A Herold
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Memory Effect ◽  
Isotope Dilution ◽  
Whole Blood ◽  
Gas Chromatography Mass Spectrometry ◽  
Pb Isotope ◽  
Stable Isotope Dilution

Abstract A stable isotope dilution gas chromatography-mass spectrometry (GC-MS) method is described for the determination of lead (Pb) in urine and whole blood. The use of lithium bis(trifluoroethyl)dithiocarbamate Pb(FDEDTC) as a chelating agent showed strong memory effect, restricting the range of Pb isotope ratios that can be measured in unknown samples. To overcome this carryover problem, we further derivatized the Pb(FDEDTC)2 chelate with 4-fluorophenyl magnesium bromide to form Pb(FC6H4)4. The sequential analyses of solutions of natural Pb and enriched 204Pb with Pb(FC6H4)4 chelate by GC-MS demonstrated no observable memory effect. Precision and accuracy of Pb isotope ratio measurements with Pb(FC6H4)4 were established, and the isotope dilution GC-MS method was validated by determining Pb concentrations in urine standards from the National Institute of Standards and Technology, urine and blood reference materials from the New York State Department of Health, and blood Pb survey samples from the College of American Pathologists.

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