UPLC-Q-TOF/MS-based screening and identification of two major bioactive components and their metabolites in normal and CKD rat plasma, urine and feces after oral administration of Rehmannia glutinosa Libosch extract

2015 ◽  
Vol 1001 ◽  
pp. 98-106 ◽  
Author(s):  
Jin-hua Tao ◽  
Min Zhao ◽  
Dong-geng Wang ◽  
Chi Yang ◽  
Guang-tong Chen ◽  
...  
Keyword(s):  
Oral Administration ◽  
Rat Plasma ◽  
Rehmannia Glutinosa ◽  
Bioactive Components ◽  
Screening And Identification ◽  
Rehmannia Glutinosa Libosch ◽  
Tof Ms

scholarly journals Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS

2021 ◽  
Vol 71 (4) ◽  
pp. 567-585
Author(s):  
Hai-Qiao Wang ◽  
Fen Lan ◽  
Yi-Han Zhang ◽  
Jin-Er Xia ◽  
Xiao-Mei Gong ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Oral Administration ◽  
Pharmacokinetic Study ◽  
Matrix Effect ◽  
Plasma Concentrations ◽  
Rat Plasma ◽  
Bioactive Components ◽  
Elimination Half ◽  
Intended Use ◽  
Tof Ms

Abstract Rhizoma Anemarrhenae is a well-known herbal medicine with saponins as its commonly regarded major bioactive components. It is essential to classify the properties of saponins which are associated with their toxicity and efficacy. In this study, 25 compounds were identified by HPLC-Q-TOF/MS in the extract of Rhizoma Anemarrhenae and 8 saponins were detected in rat plasma by HPLC-MS/MS after oral administration of this extract. These were neomangiferin, mangiferin, timosaponin E1, timosaponin E, timosaponin B-II, timosaponin B-III, timosaponin A-III and timosaponin A-I. A sensitive and accurate HPLC-MS/MS method was developed and successfully applied to a pharmacokinetic study of the abovementioned eight saponins after oral administration of the Rhizoma Anemarrhenae extract to rats. The method validation, including specificity, linearity, precision, accuracy, recovery, matrix effect and robustness, met the requirements of the intended use. The pharmacokinetic parameter, T max value, ranged from 2 to 8 h for these eight saponins whereas their elimination half-life (t 1/2) ranged from 4.06 to 9.77 h, indicating slow excretion. The plasma concentrations of these eight saponins were all very low, indicating a relatively low oral bioavailability. All these results provide support for further clinical studies.

Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q-TOF-MS/MS

2008 ◽  
Vol 46 (3) ◽  
pp. 477-490 ◽  
Author(s):  
Xijun Wang ◽  
Wenjun Sun ◽  
Hui Sun ◽  
Haitao Lv ◽  
Zeming Wu ◽  
...  
Keyword(s):  
Oral Administration ◽  
Rat Plasma ◽  
Tof Ms

Identification of chemical constituents in Rhizoma Paridis Saponins and their oral administration in rat plasma by UPLC/Q-TOF/MS

2010 ◽  
Vol 25 (6) ◽  
pp. 712-719 ◽  
Author(s):  
Shuli Man ◽  
Wenyuan Gao ◽  
Yanjun Zhang ◽  
Luqi Huang ◽  
Changxiao Liu
Keyword(s):  
Oral Administration ◽  
Chemical Constituents ◽  
Rat Plasma ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document