Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora

Crocus sativus L. is commonly used as functional food and medicinal herb in traditional Chinese medicine. In this study, the spectrum–effect relationship was established between HPLC fingerprints and in vitro antioxidant activity of saffron to improve the quality evaluation method of saffron. The fingerprints of 21 batches of saffron collected from different regions were assessed, and the data were further analyzed by chemometric methods, including similarity analysis, hierarchical clustering analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The spectrum–effect relationship between fingerprints and antioxidant effect of saffron was analyzed by grey relational analysis and partial least square methods to figure out the antioxidant component of saffron. Thirteen common peaks of 21 batches of saffron were included in the analysis, and peak 3 (picrocrocin), peak 7 (crocin I), and peak 10 (crocin II) were identified as the main active components responsible for antioxidant efficacy. Besides, a multi-index quality control method was developed for simultaneous determination of these three antioxidant components in saffron. Taken together, this study provided new strategies for the quality control and the development of new bioactive products of saffron in the future.

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Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription

Journal of Analytical Methods in Chemistry ◽

10.1155/2021/6650366 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Li Yu ◽

Yangyang Zhang ◽

Xixi Zhao ◽

Yu He ◽

Haofang Wan ◽

...

Keyword(s):

Antioxidant Activity ◽

High Performance ◽

Radical Scavenging Activity ◽

Linear Regression Analysis ◽

Radical Scavenging ◽

Multiple Linear Regression Analysis ◽

Frap Assay ◽

Antioxidant Power ◽

Effect Relationship ◽

Spectrum Effect

Yangyin Tongnao (YYTN) prescription is used as a traditional Chinese herbal formula, and it has antioxidant activity that mainly contributes in the treatment of cardiovascular and cerebrovascular diseases. However, the compounds related to its antioxidant activity are still unknown. In the present study, the fingerprints of YYTN extracts under different extraction conditions were obtained by high performance liquid chromatography (HPLC) to identify the common peaks to all the samples processed. A 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and ferric reducing antioxidant power (FRAP) assay were carried out to evaluate the antioxidant activity of the extracts. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN was assessed by Pearson product-moment correlation coefficient (PPMCC) and multiple linear regression analysis (MLRA). The results showed that peaks 5, 6, 13, 15, and 24 of the fingerprints were closely connected to antioxidant activity. Five peaks were identified: vanillic acid (P5), puerarin (P7), ferulic acid (P13), daidzein (P21), and formononetin (P23). Our study successfully established the spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN, which provided a general method for establishing quality standards with a combination of chromatography and antioxidant activity.

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Spectrum-effect relationship in antioxidant activity of Ligustri Lucidi Fructus based on DPPH, ABTS and FRAP assays

China Journal of Chinese Materia Medica ◽

10.4268/cjcmm20160917 ◽

2014 ◽

Cited By ~ 1

Keyword(s):

Antioxidant Activity ◽

Effect Relationship ◽

Spectrum Effect

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Spectrum–effect relationship between HPLC fingerprints and antioxidant activity of Angelica sinensis

Biomedical Chromatography ◽

10.1002/bmc.4707 ◽

2019 ◽

Vol 34 (2) ◽

Cited By ~ 1

Author(s):

Qin Fan ◽

Ruijing Yang ◽

Feixia Yang ◽

Pengfei Xia ◽

Lei Zhao

Keyword(s):

Antioxidant Activity ◽

Angelica Sinensis ◽

Effect Relationship ◽

Spectrum Effect

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Grouping, Spectrum–Effect Relationship and Antioxidant Compounds of Chinese Propolis from Different Regions Using Multivariate Analyses and Off-Line Anti-DPPH Assay

Molecules ◽

10.3390/molecules25143243 ◽

2020 ◽

Vol 25 (14) ◽

pp. 3243

Author(s):

Xiasen Jiang ◽

Linchen Tao ◽

Chunguang Li ◽

Mengmeng You ◽

George Q. Li ◽

...

Keyword(s):

Antioxidant Activity ◽

Caffeic Acid ◽

Linear Regression Analysis ◽

Antioxidant Properties ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Chemical Compositions ◽

Effect Relationship ◽

Spectrum Effect ◽

Chinese Propolis

49 samples of propolis from different regions in China were collected and analyzed for their chemical compositions, contents of total flavonoids (TFC), total phenolic acid (TPC) and antioxidant activity. High-performance liquid chromatography (HPLC) analysis identified 15 common components, including key marker compounds pinocembrin, 3-O-acetylpinobanksin, galangin, chrysin, benzyl p-coumarate, pinobanksin and caffeic acid phenethyl ester (CAPE). Cluster analysis (CA) and correlation coefficients (CC) analysis showed that these propolis could be divided into three distinct groups. Principal component analysis (PCA) and multiple linear regression analysis (MLRA) revealed that the contents of isoferulic acid, caffeic acid, CAPE, 3,4-dimethoxycinnamic acid, chrysin and apigenin are closely related to the antioxidant properties of propolis. In addition, eight peak areas decreased after reacting with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radicals, indicating that these compounds have antioxidant activity. The results indicate that the grouping and spectrum–effect relationship of Chinese propolis are related to their chemical compositions, and several compounds may serve as a better marker for the antioxidant activity of Chinese propolis than TFC and TPC. The findings may help to develop better methods to evaluate the quality of propolis from different geographic origins.

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