Optimization of Extraction Process and the Antioxidant Activity Spectrum‐effect Relationship of Angelica dahurica

2022 ◽  
Author(s):  
Hanting Yang ◽  
Qian Li
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Angelica Dahurica ◽  
Effect Relationship ◽  
Activity Spectrum ◽  
Relationship Of ◽  
Spectrum Effect

scholarly journals Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Ya You ◽  
Zijin Xu ◽  
Qingrou Zhong ◽  
Lin Zhu ◽  
Susu Lin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Evaluation Method ◽  
Control Method ◽  
Partial Least Square ◽  
Least Square ◽  
Crocus Sativus ◽  
Effect Relationship ◽  
Spectrum Effect

Crocus sativus L. is commonly used as functional food and medicinal herb in traditional Chinese medicine. In this study, the spectrum–effect relationship was established between HPLC fingerprints and in vitro antioxidant activity of saffron to improve the quality evaluation method of saffron. The fingerprints of 21 batches of saffron collected from different regions were assessed, and the data were further analyzed by chemometric methods, including similarity analysis, hierarchical clustering analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The spectrum–effect relationship between fingerprints and antioxidant effect of saffron was analyzed by grey relational analysis and partial least square methods to figure out the antioxidant component of saffron. Thirteen common peaks of 21 batches of saffron were included in the analysis, and peak 3 (picrocrocin), peak 7 (crocin I), and peak 10 (crocin II) were identified as the main active components responsible for antioxidant efficacy. Besides, a multi-index quality control method was developed for simultaneous determination of these three antioxidant components in saffron. Taken together, this study provided new strategies for the quality control and the development of new bioactive products of saffron in the future.

Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora

2020 ◽  
Vol 1140 ◽  
pp. 121970 ◽  
Author(s):  
Yinrui Chen ◽  
Guoji Pan ◽  
Wenfen Xu ◽  
Qingwen Sun ◽  
Bo Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Effect Relationship ◽  
Relationship Study ◽  
Spectrum Effect

scholarly journals Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Li Yu ◽  
Yangyang Zhang ◽  
Xixi Zhao ◽  
Yu He ◽  
Haofang Wan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Linear Regression Analysis ◽  
Radical Scavenging ◽  
Multiple Linear Regression Analysis ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Effect Relationship ◽  
Spectrum Effect

Yangyin Tongnao (YYTN) prescription is used as a traditional Chinese herbal formula, and it has antioxidant activity that mainly contributes in the treatment of cardiovascular and cerebrovascular diseases. However, the compounds related to its antioxidant activity are still unknown. In the present study, the fingerprints of YYTN extracts under different extraction conditions were obtained by high performance liquid chromatography (HPLC) to identify the common peaks to all the samples processed. A 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and ferric reducing antioxidant power (FRAP) assay were carried out to evaluate the antioxidant activity of the extracts. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN was assessed by Pearson product-moment correlation coefficient (PPMCC) and multiple linear regression analysis (MLRA). The results showed that peaks 5, 6, 13, 15, and 24 of the fingerprints were closely connected to antioxidant activity. Five peaks were identified: vanillic acid (P5), puerarin (P7), ferulic acid (P13), daidzein (P21), and formononetin (P23). Our study successfully established the spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN, which provided a general method for establishing quality standards with a combination of chromatography and antioxidant activity.

scholarly journals Dynamic Change of Secondary Metabolites and spectrum-effect relationship of Malus halliana Koehne flowers during blooming

2018 ◽  
Vol 16 (1) ◽  
pp. 362-370 ◽  
Author(s):  
Lili Cui ◽  
Nan He ◽  
Xiaofeng Zhang ◽  
Shiming Li ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Dynamic Change ◽  
Inhibitory Effect ◽  
Partial Least Square ◽  
Least Square ◽  
Effect Relationship ◽  
Relationship Of ◽  
Spectrum Effect

AbstractMalus halliana Koehne flowers have been used as a Chinese traditional medicine to treat metrorrhagia. In this study, the dynamic changes in its secondary metabolites and spectrum-effect relationship of inhibition on α-glucosidase during blooming were investigated. The changes in the contents of three flavonoids (phloretin-4’-O-glycosidase, afzeloside, and 3-hydroxyphloridzin) were determined by high performance liquid chromatography (HPLC) and changes in inhibitory effect on α-glucosidase were evaluated in vitro. Then, spectrum-effect relationship was evaluated by partial least square method. The results indicated that the contents of three flavonoids and inhibition of α-glucosidase activity in vitro showed a fluctuating downward trend, thereinto, the maximum contents of phloretin-4’-O-glycosidase, afzeloside, and 3-hydroxyphloridzin reached 157.43±0.36, 17.27±0.06 and 22.67±0.35 (mg/g), respectively. In spectrum-effect relationship assay, matched 40 mutual peaks, thereinto, P2, P5, P6-P12, P14 (3-hydroxyphloridzin), P16-P19, P20 (phloretin-4’-O-glycosidase), P24, P26, P29, P31, P33, P34, P36, P39 and P40 were positively correlated to inhibitory effect on α-glucosidase in vitro. P1, P3, P4, P13, P15, P21-P23, P27, P28, P30 (afzeloside), P32, P35, P37 and P38 were negatively related to inhibitory effect on α-glucosidase in vitro.

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