scholarly journals Valorisation of deinking sludge as a substrate for lignocellulolytic enzymes production by Pleurotus ostreatus

2018 ◽  
Vol 197 ◽  
pp. 253-263 ◽  
Author(s):  
Maša Vodovnik ◽  
Katja Vrabec ◽  
Patrick Hellwig ◽  
Dirk Benndorf ◽  
Mija Sežun ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Lignocellulolytic Enzymes

Pola Aktivitas Enzim Lakase, Selulase, dan Xilanase Pada Masa Pertumbuhan Budidaya Jamur Tiram Putih [Pleurotus ostreatus (Jacq.) P. Kumm.]

2021 ◽  
Vol 17 (2) ◽  
pp. 145-151
Author(s):  
Iwan Saskiawan ◽  
◽  
Nunuk Widhyastuti ◽  
Kasirah ◽  
Dodi Sutardi ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Similar Pattern ◽  
Laccase Activity ◽  
Xylanase Activity ◽  
Edible Mushroom ◽  
Lignocellulolytic Enzymes ◽  
Corn Flour ◽  
Mushroom Production ◽  
Main Substrate

Recently, Pleurotus ostreatus has become the most cultivated edible mushroom in Indonesia because of the simple in cultivation. Pleurotus ostreatus easy to grow in a medium contained sawdust as a main substrate, with the addition of rice brand, corn flour, lime and gypsum. Mushroom production is bioconversion process which involves the activity of lignocellulolytic enzymes such as laccase, cellulase, and xylanase. The aim of this study was to reveal the pattern of laccase, cellulase, and xylanase activity during the growth of P. ostreatus on the sawdust medium. The results show that the highest laccase activity was obtained on the 15th day after spawn inoculation (ASI) of 0.66 Unit and decline sharply after the day of 45th ASI until the day of 75th ASI. Furthermore, the similar pattern with difference on the time incubation was obtained on the activity of cellulase and xylanase. It was decrease after the day 30th ASI. The highest activity of cellulase was 0.51 Unit on 30th day of ASI and decrease gradually until the day of 75th ASI. Furthermore, the highest activity of xylanase was on 30th day ASI of 0.34 Unit and decrease sharply until the day of 75th.

scholarly journals Solid-state fermentation on poplar sawdust and corncob wastes for lignocellulolytic enzymes by different Pleurotus ostreatus strains

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 4982-4995
Author(s):  
Mei-Ling Han ◽  
Qi An ◽  
Sai-Fei He ◽  
Xiao-Lin Zhang ◽  
Ming-Hui Zhang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Wild Strain ◽  
Xylanase Activity ◽  
Lignocellulosic Materials ◽  
Lignocellulolytic Enzymes ◽  
Poplar Sawdust

Solid state fermentation with different lignocellulolytic materials as inducers was used for lignocellulolytic enzyme production in this study. Pleurotus ostreatus strains were assessed by measuring laccase, CMCase, and xylanase activities. The secretion potential of the lignocellulolytic enzymes by wild and cultivated strains was analyzed for the first time. The wild and cultivated strain showed their unique capacities for secreting lignocellulolytic enzymes on solid-state fermentation with different lignocellulosic materials. The wild P. ostreatus strain preferred corncob for the secretion of laccase and xylanase activity, but the cultivated strain preferred poplar sawdust. The wild strain and cultivated strain showed a consistent preference for poplar sawdust for the secretion of CMCase activity. The wild strain was advantageous because it achieved the maximum hydrolytic enzyme activities within a short time period. Poplar sawdust and corncob were conducive to laccase secretion by the wild or cultivated strains and the rapid accumulation of laccase on solid-state fermentation. Additionally, continuous, stable laccase production was an extremely important advantage by solid-state fermentation of poplar sawdust, particularly in the wild strain. These findings are helpful in selecting the appropriate strain that corresponds to suitable lignocellulosic materials. The optimization of integrated industrial lignocellulolytic enzyme production can also be achieved.

scholarly journals Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

2014 ◽  
Vol 20 (1) ◽  
pp. 117 ◽  
Author(s):  
Balkys Quevedo-Hidalgo ◽  
Paulo Cesar Narváez-Rincón ◽  
Aura Marina Pedroza-Rodríguez ◽  
Mario Enrique Velásquez-Lozano
Keyword(s):  
Pleurotus Ostreatus ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
Cost Effective ◽  
Industrial Sector ◽  
Raw Material ◽  
Manganese Sulfate ◽  
Lignocellulolytic Enzymes ◽  
Waste Products ◽  
Balance Of Trade

Floriculture is a vital agro-industrial sector in the Colombian economy; the export of flowers positively impacts employment and the balance of trade. However, this industry could negatively impact the environment if its waste products are not handled properly. These flower residues, rich in lignin, hemicellulose and cellulose, could be a cost-effective raw material to produce enzymes. Here, we evaluate the production of lignocellulolytic enzymes by degradation of Chrysanthemum and Rosa residues using Pleurotus ostreatus and manganese sulfate and copper sulfate as inductors. From the two residues, we obtained laccase, manganese peroxidase, endoglucanase, exoglucanase, and β-glucosidase. The use of inductors, favored all enzyme activities except for β-glucosidase. The enzymes that displayed the highest activity were laccase (4,693.4 U/L and 2,640 U/L from the residues of Chrysanthemum and Rosa respectively) and β-glucosidase (9,513 U/L and 6,811.9 U/L). The enzyme that showed the lowest activity was endoglucanase (11.5 U/L and 15.4 U/L). Under the conditions evaluated, the best substrate for enzyme production is <em>Chrysanthemum</em> wastes; the extracts obtained had higher enzymatic activity than the extracts from Rosa residues.

Пищевая, лечебная и экологическая ценность грибов Pleurotus ostreatus

2010 ◽  
Vol 18 (1) ◽  
pp. 3-9
Author(s):  
O. M. Alekseenko ◽  
T. M. Polishko ◽  
A. I. Vinnikov
Keyword(s):  
Pleurotus Ostreatus

Проанализированы данные литературы, характеризующие пищевые, лечебные и экологические свойства гриба Рleurotus ostreatus (вешенка обыкновенная). Вешенка – полноценный продукт питания, обеспечивающий потребность организма человека в белках, углеводах, жирах, витаминах и минеральных солях. В белках плодовых тел вешенки содержится 18 аминокислот, восемь из которых незаменимые (изолейцин, лейцин, лизин, метионин, фенилаланин, триптофан, треонин, валин). Лечебная ценность грибов характеризуется содержанием водорастворимых (тиамин В1, рибофлавин В2, ниацин В5, РР, пиридоксин В6, биотин В7, аскорбиновая и пантотеновая кислота) и жирорастворимых (кальциферол, эргостерол, токоферол) витаминов. Отмечена возможность значительного повышения эффективности использования отходов сельского хозяйства за счет получения плодовых тел, а также последующего применения субстрата после плодоношения в растениеводстве и животноводстве. 

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