Pola Aktivitas Enzim Lakase, Selulase, dan Xilanase Pada Masa Pertumbuhan Budidaya Jamur Tiram Putih [Pleurotus ostreatus (Jacq.) P. Kumm.]

Recently, Pleurotus ostreatus has become the most cultivated edible mushroom in Indonesia because of the simple in cultivation. Pleurotus ostreatus easy to grow in a medium contained sawdust as a main substrate, with the addition of rice brand, corn flour, lime and gypsum. Mushroom production is bioconversion process which involves the activity of lignocellulolytic enzymes such as laccase, cellulase, and xylanase. The aim of this study was to reveal the pattern of laccase, cellulase, and xylanase activity during the growth of P. ostreatus on the sawdust medium. The results show that the highest laccase activity was obtained on the 15th day after spawn inoculation (ASI) of 0.66 Unit and decline sharply after the day of 45th ASI until the day of 75th ASI. Furthermore, the similar pattern with difference on the time incubation was obtained on the activity of cellulase and xylanase. It was decrease after the day 30th ASI. The highest activity of cellulase was 0.51 Unit on 30th day of ASI and decrease gradually until the day of 75th ASI. Furthermore, the highest activity of xylanase was on 30th day ASI of 0.34 Unit and decrease sharply until the day of 75th.

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Solid-state fermentation on poplar sawdust and corncob wastes for lignocellulolytic enzymes by different Pleurotus ostreatus strains

BioResources ◽

10.15376/biores.15.3.4982-4995 ◽

2020 ◽

Vol 15 (3) ◽

pp. 4982-4995

Author(s):

Mei-Ling Han ◽

Qi An ◽

Sai-Fei He ◽

Xiao-Lin Zhang ◽

Ming-Hui Zhang ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Pleurotus Ostreatus ◽

Enzyme Production ◽

Hydrolytic Enzyme ◽

Wild Strain ◽

Xylanase Activity ◽

Lignocellulosic Materials ◽

Lignocellulolytic Enzymes ◽

Poplar Sawdust

Solid state fermentation with different lignocellulolytic materials as inducers was used for lignocellulolytic enzyme production in this study. Pleurotus ostreatus strains were assessed by measuring laccase, CMCase, and xylanase activities. The secretion potential of the lignocellulolytic enzymes by wild and cultivated strains was analyzed for the first time. The wild and cultivated strain showed their unique capacities for secreting lignocellulolytic enzymes on solid-state fermentation with different lignocellulosic materials. The wild P. ostreatus strain preferred corncob for the secretion of laccase and xylanase activity, but the cultivated strain preferred poplar sawdust. The wild strain and cultivated strain showed a consistent preference for poplar sawdust for the secretion of CMCase activity. The wild strain was advantageous because it achieved the maximum hydrolytic enzyme activities within a short time period. Poplar sawdust and corncob were conducive to laccase secretion by the wild or cultivated strains and the rapid accumulation of laccase on solid-state fermentation. Additionally, continuous, stable laccase production was an extremely important advantage by solid-state fermentation of poplar sawdust, particularly in the wild strain. These findings are helpful in selecting the appropriate strain that corresponds to suitable lignocellulosic materials. The optimization of integrated industrial lignocellulolytic enzyme production can also be achieved.

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Utilization of coconut water as inducing substance in the cultivation of Pleurotus ostreatus

Jurnal AGROSAINS dan TEKNOLOGI ◽

10.24853/jat.4.1.25-30 ◽

2019 ◽

Vol 4 (1) ◽

pp. 25

Author(s):

Elfarisna Elfarisna ◽

Iwan Saskiawan

Keyword(s):

Developing Countries ◽

Pleurotus Ostreatus ◽

Fruit Body ◽

Edible Mushroom ◽

The Other ◽

Coconut Water ◽

Edible Mushrooms ◽

Body Formation ◽

Fruit Body Formation ◽

Main Substrate

Cultivation of edible mushrooms has been evaluated as an effective way for increasing income of people in developing countries. Pleurotus ostreatus, well known as an oyster mushroom is the most popular edible mushroom in Indonesia. They usually is cultivated using a sawdust as a main substrate. The aim of this research is to reveal the addition of coconut water, the byproduct of traditionally coconut process as an inducing substance in P. ostreatus cultivation. The results shown that the addition 25% (v/v) of coconut water in substrate of P. ostreatus induce the growth of mycelium. On the other hand, the addition of 50% (v/v) of coconut water affect in the day of fruit body formation become faster as well as increase the number of pileus (stalk) and the weight of fruit body.

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A heteropolysaccharide from aqueous extract of an edible mushroom, Pleurotus ostreatus cultivar: structural and biological studies

Carbohydrate Research ◽

10.1016/j.carres.2010.10.026 ◽

2011 ◽

Vol 346 (2) ◽

pp. 366-372 ◽

Cited By ~ 34

Author(s):

Kankan K. Maity ◽

Sukesh Patra ◽

Biswajit Dey ◽

Sanjoy K. Bhunia ◽

Soumitra Mandal ◽

...

Keyword(s):

Aqueous Extract ◽

Pleurotus Ostreatus ◽

Edible Mushroom ◽

Biological Studies

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Growth and yield impact of oyster mushroom Pleurotus ostreatus (Jacq P. Kumm) cultivated on different agricultural wastes

Journal of Bioscience and Agriculture Research ◽

10.18801/jbar.270121.272 ◽

2021 ◽

Vol 27 (01) ◽

pp. 2225-2233

Author(s):

M. O. Agba ◽

◽

A. A. Markson ◽

J. O. Oni ◽

G. A. Bassey ◽

...

Keyword(s):

Pleurotus Ostreatus ◽

Growth Parameters ◽

Total Yield ◽

Fruit Body ◽

Food Product ◽

Dry Weight ◽

Edible Mushroom ◽

Growth And Yield ◽

Biological Efficiency ◽

Essential Food

Pleurotus ostreatus, an edible mushroom is an essential food product. Recognised as one of the cheapest sources of proteins, their high nutritional and anti-nutritional properties are of immense importance to humans. The present study evaluated the effect of sawdust and dried plantain leaves on the growth and yield of Pleurotus ostreatus. Sawdust and dried plantain leaves were composted into seven substrates; as single substrates and at different combinations. The composted substrates include sawdust 100 % (M0), Dry plantain leaves 100% (M1), Dry plantain leaves + sawdust at the ratio of 1:4 (M2), 2:3 (M3), 3:2 (M4), 1:1 (M5) and 4:1(M6). Growth parameters of interest assessed flush include mycelia running rate (MRR), area of pileus, length of the stipe, the girth of the stipe, fresh weight and dry weight, number of fruit body, total yield and biological efficiency. At the end of the three weeks spawn running period, M0 was overall best in supporting mycelia running rate with a mean MRR of 16.00 cm. M1 substrates produced mushroom with longer and bigger stipes (7.17 cm) at the first flush. A higher number of fruit bodies (82.66), total yield (130.35 g), and biological efficiency (43.45 g) were all seen in M0 substrates. Sawdust at 100 % (M0) proved to be the best substrate for the cultivation of Pleurotus ostreatus.

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Enhancement of The Edible Mushroom Pleurotus Ostreatus Yield via A New Liquid Fermentation Tank

Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016) ◽

10.2991/bep-16.2017.11 ◽

2017 ◽

Author(s):

Xiaoyu DUAN ◽

Yu HU ◽

Yuxian YOU ◽

Qianqian TANG ◽

Yiwen LI ◽

...

Keyword(s):

Pleurotus Ostreatus ◽

Edible Mushroom ◽

Liquid Fermentation ◽

Fermentation Tank

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Valorisation of deinking sludge as a substrate for lignocellulolytic enzymes production by Pleurotus ostreatus

Journal of Cleaner Production ◽

10.1016/j.jclepro.2018.06.163 ◽

2018 ◽

Vol 197 ◽

pp. 253-263 ◽

Cited By ~ 1

Author(s):

Maša Vodovnik ◽

Katja Vrabec ◽

Patrick Hellwig ◽

Dirk Benndorf ◽

Mija Sežun ◽

...

Keyword(s):

Pleurotus Ostreatus ◽

Lignocellulolytic Enzymes

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MOLECULAR ANALYSIS OF LIGNOCELLULOLYTIC ENZYMES OF THE EDIBLE MUSHROOM AGARICUS BISPORUS

Biotechnology in Pulp and Paper Manufacture ◽

10.1016/b978-0-409-90192-4.50072-8 ◽

1990 ◽

pp. 659-666 ◽

Cited By ~ 2

Author(s):

D.A. Wood ◽

C. Perry ◽

C.F. Thurston ◽

S.E. Matcham ◽

K. Dudley ◽

...

Keyword(s):

Molecular Analysis ◽

Agaricus Bisporus ◽

Edible Mushroom ◽

Lignocellulolytic Enzymes

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Comparative Study of Pleurotus ostreatus Mushroom Grown on Modified PAN Nanofiber Mats

Nanomaterials ◽

10.3390/nano9030475 ◽

2019 ◽

Vol 9 (3) ◽

pp. 475 ◽

Cited By ~ 9

Author(s):

Lilia Sabantina ◽

Franziska Kinzel ◽

Thomas Hauser ◽

Astrid Többer ◽

Michaela Klöcker ◽

...

Keyword(s):

Comparative Study ◽

Growth Rates ◽

Pleurotus Ostreatus ◽

Edible Mushroom ◽

Fungal Mycelium ◽

Specific Substrate ◽

Biotechnological Applications ◽

Mushroom Species ◽

Nanofiber Mats ◽

Pan Nanofiber

Pleurotus ostreatus is a well-known edible mushroom species which shows fast growth. The fungus can be used for medical, nutritional, filter, or packaging purposes. In this study, cultivation experiments were carried out with Pleurotus ostreatus growing on polyacrylonitrile (PAN) nanofiber mats in the presence of saccharose and Lutrol F68. The aim of this study was to find out whether modified PAN nanofiber mats are well suited for the growth of fungal mycelium, to increase growth rates and to affect mycelium fiber morphologies. Our results show that Pleurotus ostreatus mycelium grows on nanofiber mats in different morphologies, depending on the specific substrate, and can be used to produce a composite from fungal mycelium and nanofiber mats for biomedical and biotechnological applications.

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