Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering

2009 ◽  
Vol 139 (1) ◽  
pp. 2-7 ◽  
Author(s):  
Ji Suk Choi ◽  
Hyun-Jin Yang ◽  
Beob Soo Kim ◽  
Jae Dong Kim ◽  
Jun Young Kim ◽  
...  
2017 ◽  
Vol 12 (3) ◽  
pp. 035007 ◽  
Author(s):  
Alisan Kayabolen ◽  
Dilek Keskin ◽  
Andac Aykan ◽  
Yıldırım Karslıoglu ◽  
Fatih Zor ◽  
...  

RSC Advances ◽  
2016 ◽  
Vol 6 (58) ◽  
pp. 53034-53042 ◽  
Author(s):  
Yongzhou Lu ◽  
Chuanlong Jia ◽  
Bo Bi ◽  
Liang Chen ◽  
Yiqun Zhou ◽  
...  

This study provides a novel method in injectable tissue engineering which contains porcine extracellular matrix (ECM) powder scaffolds and stromal-vascular fraction (SVF) cells.


2020 ◽  
Vol 6 (3) ◽  
pp. 410-413
Author(s):  
Petra J. Kluger ◽  
Svenja Nellinger ◽  
Simon Heine ◽  
Ann-Cathrin Volz

AbstractThe extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial biochemical signals that influence cell adhesion, morphology, proliferation and differentiation. Next to native ECM from mature tissue, ECM can also be obtained from the in vitro culture of cells. In this study, we aimed to highlight the supporting effect of cell-derived- ECM (cdECM) on adipogenic differentiation. ASCs were seeded on top of cdECM from ASCs (scdECM) or pre-adipocytes (acdECM). The impact of ECM on cellular activity was determined by LDH assay, WST I assay and BrdU assay. A supporting effect of cdECM substrates on adipogenic differentiation was determined by oil red O staining and subsequent quantification. Results revealed no effect of cdECM substrates on cellular activity. Regarding adipogenic differentiation a supporting effect of cdECM substrates was obtained compared to control. With these results, we confirm cdECM as a promising biomaterial for adipose tissue engineering.


Biomaterials ◽  
2007 ◽  
Vol 28 (26) ◽  
pp. 3834-3842 ◽  
Author(s):  
Lauren Flynn ◽  
Glenn D. Prestwich ◽  
John L. Semple ◽  
Kimberly A. Woodhouse

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