scholarly journals Skeletal and Adipose Tissue Engineering with Adipose-Derived Stromal Cells

10.5772/22971 ◽  
2011 ◽  
Author(s):  
Jeong S ◽  
Emily R ◽  
Daniel Montoro ◽  
Benjamin Levi ◽  
Michael T.
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Adipose Tissue Engineering ◽  
Adipose Derived Stromal Cells

Adipose Tissue Engineering by Human Adipose-Derived Stromal Cells

2006 ◽  
Vol 183 (3) ◽  
pp. 133-140 ◽  
Author(s):  
Liu Hong ◽  
Ioana A. Peptan ◽  
Aylin Colpan ◽  
Joseph L. Daw
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Adipose Tissue Engineering ◽  
Adipose Derived Stromal Cells

Adipose-tissue engineering: Taking advantage of the properties of human adipose-derived stem/stromal cells

2009 ◽  
Vol 57 (4) ◽  
pp. 309-317 ◽  
Author(s):  
M. Vallée ◽  
J.-F. Côté ◽  
J. Fradette
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Adipose Tissue Engineering

Perfusion bioreactor culture of adipose-derived stromal cells on decellularized adipose tissue scaffolds enhances in vivo regeneration

2020 ◽  
Author(s):  
Tim Tian Y. Han ◽  
Lauren E. Flynn
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Unmet Need ◽  
Perfusion Bioreactor ◽  
Bioreactor Culture ◽  
Adipose Tissue Engineering ◽  
Adipose Derived Stromal Cells ◽  
The Impact

AbstractAdipose tissue engineering holds promise to address the unmet need in plastic and reconstructive surgery for strategies that promote the stable and predictable regeneration of adipose tissue for volume augmentation applications. Previous studies have demonstrated that decellularized adipose tissue (DAT) scaffolds can provide a pro-adipogenic microenvironment, and that seeding with adipose-derived stromal cells (ASCs) can enhance in vivo angiogenesis and adipogenesis within DAT implants. Recognizing that bioreactor systems can promote cell expansion and infiltration on tissue-engineered scaffolds, this study evaluated the effects of culturing human ASCs on DAT scaffolds within a perfusion bioreactor. Using this system, the impact of both shear stress stimulation and hypoxic preconditioning were explored in vitro and in vivo. Initial studies compared the effects of 14 days of culture within the perfusion bioreactor under 2% O2 or ~20% O2 on human ASC expansion and hypoxia inducible factor 1 alpha (HIF-1α) expression in vitro relative to static cultured controls. The findings indicated that culturing within the bioreactor under 2% O2 significantly increased ASC proliferation on the DAT, with a higher cell density observed in the scaffold periphery. HIF-1α expression was significantly higher when the scaffolds were cultured under 2% O2. Subsequent characterization in a subcutaneous implant model in athymic nude mice revealed that in vivo angiogenesis and adipogenesis were markedly enhanced when the ASCs were cultured on the DAT within the perfusion bioreactor under 2% O2 for 14 days prior to implantation relative to the other culture conditions, as well as additional freshly-seeded and unseeded DAT control groups. Overall, dynamic culture within the perfusion bioreactor system under hypoxia represents a promising approach for preconditioning ASCs on DAT scaffolds to enhance their capacity to stimulate blood vessel formation and infiltration, as well as host-derived adipose tissue regeneration.

Composite Scaffolds of Black Phosphorus Nanosheets and Gelatin with Controlled Pore Structures for Photothermal Cancer Therapy and Adipose Tissue Engineering

Biomaterials ◽  
2021 ◽  
pp. 120923
Author(s):  
Linawati Sutrisno ◽  
Huajian Chen ◽  
Yazhou Chen ◽  
Toru Yoshitomi ◽  
Naoki Kawazoe ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Cancer Therapy ◽  
Black Phosphorus ◽  
Composite Scaffolds ◽  
Adipose Tissue Engineering ◽  
Pore Structures

Adipose tissue engineering with naturally derived scaffolds and adipose-derived stem cells

Biomaterials ◽  
2007 ◽  
Vol 28 (26) ◽  
pp. 3834-3842 ◽  
Author(s):  
Lauren Flynn ◽  
Glenn D. Prestwich ◽  
John L. Semple ◽  
Kimberly A. Woodhouse
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Adipose Derived Stem Cells ◽  
Adipose Tissue Engineering

Osteogenesis for postoperative temporal bone defects using human ear adipose-derived stromal cells and tissue engineering: An animal model study

2017 ◽  
Vol 105 (12) ◽  
pp. 3493-3501 ◽  
Author(s):  
Yeon Ju Kim ◽  
Seung Gu Park ◽  
Beomyong Shin ◽  
Jangho Kim ◽  
Seung Won Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Animal Model ◽  
Temporal Bone ◽  
Stromal Cells ◽  
Bone Defects ◽  
Adipose Derived Stromal Cells ◽  
Model Study ◽  
Animal Model Study ◽  
Human Ear
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