scholarly journals Cell-derived Extracellular Matrix - a promising Biomaterial for in vitro-vascularization in Adipose Tissue Engineering

Authorea ◽  
2020 ◽  
Author(s):  
Svenja Nellinger ◽  
Isabelle Schmidt ◽  
Simon Heine ◽  
Ann Cathrin Volz ◽  
Petra Kluger
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Adipose Tissue Engineering

scholarly journals Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation

2020 ◽  
Vol 6 (3) ◽  
pp. 410-413
Author(s):  
Petra J. Kluger ◽  
Svenja Nellinger ◽  
Simon Heine ◽  
Ann-Cathrin Volz
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipogenic Differentiation ◽  
Cellular Activity ◽  
Adipose Tissue Engineering ◽  
Physical Support ◽  
Supporting Effect ◽  
Brdu Assay ◽  
The Impact

AbstractThe extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial biochemical signals that influence cell adhesion, morphology, proliferation and differentiation. Next to native ECM from mature tissue, ECM can also be obtained from the in vitro culture of cells. In this study, we aimed to highlight the supporting effect of cell-derived- ECM (cdECM) on adipogenic differentiation. ASCs were seeded on top of cdECM from ASCs (scdECM) or pre-adipocytes (acdECM). The impact of ECM on cellular activity was determined by LDH assay, WST I assay and BrdU assay. A supporting effect of cdECM substrates on adipogenic differentiation was determined by oil red O staining and subsequent quantification. Results revealed no effect of cdECM substrates on cellular activity. Regarding adipogenic differentiation a supporting effect of cdECM substrates was obtained compared to control. With these results, we confirm cdECM as a promising biomaterial for adipose tissue engineering.

In Vitro Adipose Tissue Engineering Using an Electrospun Nanofibrous Scaffold

2008 ◽  
Vol 61 (5) ◽  
pp. 566-571 ◽  
Author(s):  
Rabie M. Shanti ◽  
Sasa Janjanin ◽  
Wan-Ju Li ◽  
Leon J. Nesti ◽  
Michael B. Mueller ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Nanofibrous Scaffold ◽  
Adipose Tissue Engineering

scholarly journals VEGF heparinized-decellularized adipose tissue scaffolds enhance tissue engineering vascularization in vitro

RSC Advances ◽  
2018 ◽  
Vol 8 (59) ◽  
pp. 33614-33624
Author(s):  
Mei Song ◽  
Yu Zhou ◽  
Yi Liu
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Tissue Scaffolds ◽  
Adipose Tissue Engineering

Scaffolds based on decellularized adipose tissue (DAT) are gaining popularity in the adipose tissue engineering field due to their high biocompatibility and vascularizing properties.

scholarly journals Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial

2021 ◽  
Author(s):  
Svenja Nellinger ◽  
Ivana Mrsic ◽  
Silke Keller ◽  
Simon Heine ◽  
Alexander Southan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Cultured Cells ◽  
Structural Characteristics ◽  
Human Adipose Tissue ◽  
Minimal Invasive ◽  
Healthcare Applications ◽  
Decellularized Extracellular Matrix

Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively towards their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells (ASCs), towards their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans (sGAGs) compared to cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results.

The tissue-engineered breast

2021 ◽  
pp. 1115-1120
Author(s):  
Wayne Morrison
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stem Cell Differentiation ◽  
Vascular Pedicle ◽  
Human Trial ◽  
Fat Transfer ◽  
Adipose Tissue Engineering ◽  
Proof Of Principle

The adipose tissue engineering paradigm of in vitro cell-seeded scaffolds subsequently implanted in vivo failed because of inadequate vascularization. Consequently, entirely in vivo models of tissue engineering are being trialled where angiogenic growth is stimulated in unison with expansion of implanted cells and matrices. In animals, impressive amounts of fat and fibroblastic tissue have been grown by matrix induction of preadipocytes or by redirecting a vascular pedicle with fat into a sealed chamber space and proof of principle shown in a human trial. The models are cumbersome limiting clinical translation and currently direct fat transfer by injection is simpler. Unlike true tissue engineering there is, however, no net gain of tissue and even when ‘successful’ it is debated whether the graft survives or is replaced by newly regenerated adipocytes. Research focuses on stem cell differentiation, cell survival, and matrix and biomechanical manipulations.

Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering

2009 ◽  
Vol 139 (1) ◽  
pp. 2-7 ◽  
Author(s):  
Ji Suk Choi ◽  
Hyun-Jin Yang ◽  
Beob Soo Kim ◽  
Jae Dong Kim ◽  
Jun Young Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Cell Delivery ◽  
Adipose Tissue Engineering

Adipose Tissue Engineering Based on Mesenchymal Stem Cells and Basic Fibroblast Growth Factor in Vitro

2005 ◽  
Vol 11 (11-12) ◽  
pp. 1840-1851 ◽  
Author(s):  
Markus Neubauer ◽  
Michael Hacker ◽  
Petra Bauer-Kreisel ◽  
Barbara Weiser ◽  
Claudia Fischbach ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Adipose Tissue Engineering
Sign in / Sign up

Export Citation Format

Share Document