Preparation of microporous and macroporous biphasic calcium phosphate scaffolds modified via a biomimetic method for bone tissue engineering applications

2013 ◽  
Vol 172 (1) ◽  
pp. e132
Author(s):  
Lei Nie ◽  
Qi Yang ◽  
Jinping Suo
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Engineering Applications ◽  
Biomimetic Method

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

Preparation and characterization of nano biphasic calcium phosphate/poly-L-lactide composite scaffold

2016 ◽  
Vol 23 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Weizhong Yang ◽  
Yong Yi ◽  
Yuan Ma ◽  
Li Zhang ◽  
Jianwen Gu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Cell Viability ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Composite Scaffold ◽  
Pore Wall ◽  
Tissue Engineering Scaffold ◽  
Plla Scaffold

AbstractNano biphasic calcium phosphate (BCP) particles were synthesized using the sol-gel method. As-prepared BCP particles were combined with poly-L-lactide (PLLA) to fabricate nano-BCP/PLLA composite scaffold through a series of processing steps containing solvent self-diffusion, hot-pressing, and particulate leaching. The composite had a suitable porous structure for bone tissue engineering scaffold. In comparison, micro-BCP/PLLA scaffold was studied as well. Nano-BCP particles were distributed homogeneously in the PLLA matrix, and much more tiny crystallites exposed on the surface of the pore wall. Due to the finer inorganic particle distribution in the PLLA phase and the larger area of the bioactive phase exposed in the pore wall surface, nano-BCP/PLLA scaffold had enhanced compressive strength, good bioactivity, and superior cell viability. A nonstoichiometric apatite layer could be rapidly formed on the surface of nano- BCP/PLLA when soaked in simulated body fluid. The MG-63 cell viability of nano-BCP/PLLA scaffold is significantly higher than that of micro-BCP/PLLA scaffold. Therefore, nano-BCP/PLLA composite may be a suitable alternative for bone tissue engineering scaffold.

Effect of zirconia-mullite incorporated biphasic calcium phosphate/biopolymer composite scaffolds for bone tissue engineering

2020 ◽  
Vol 6 (5) ◽  
pp. 055004
Author(s):  
Tanawut Rittidach ◽  
Tanatsaparn Tithito ◽  
Panan Suntornsaratoon ◽  
Narattaphol Charoenphandhu ◽  
Jirawan Thongbunchoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Composite Scaffolds

Fully Interconnected Globular Porous Biphasic Calcium Phosphate Ceramic Scaffold Facilitates Osteogenic Repair

2007 ◽  
Vol 361-363 ◽  
pp. 119-122 ◽  
Author(s):  
J.H. Lim ◽  
J.H. Park ◽  
Eui Kyun Park ◽  
Hae Jung Kim ◽  
Il Kyu Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Substitute ◽  
Biphasic Calcium Phosphate ◽  
Bone Defects ◽  
Calcium Phosphate Ceramic ◽  
Ceramic Scaffold ◽  
Porous Biphasic Calcium Phosphate

An appropriate scaffold, which provides structural support for transplanted cells and acts as a vehicle for the delivery of biologically active molecules, is critical for tissue engineering. We developed a fully interconnected globular porous biphasic calcium phosphate ceramic scaffold by adopting a foaming method, and evaluated its efficiency as a bone substitute and a scaffold for bone tissue engineering by in vitro and in vivo biocompatible analysis and its osteogenic healing capacity in rat tibial bone defects. They have spherical pores averaging 400um in diameter and interconnecting interpores averaging 70um in diameter with average 85% porosity. They elicited no cytotoxicity and noxious effect on cellular proliferation and osteoblastic differentiation during the cell-scaffold construct formation. Also the bone defects grafted with fully interconnected globular porous biphasic calcium phosphate ceramic blocks revealed excellent bone healing within 3 weeks. These findings suggest that the fully interconnected porous biphasic calcium phosphate scaffold formed by the foaming method can be a promising bone substitute and a scaffold for bone tissue engineering.

Evaluation of Chitosan/Biphasic Calcium Phosphate Scaffolds for Maxillofacial Bone Tissue Engineering

2008 ◽  
Vol 269 (1) ◽  
pp. 100-105 ◽  
Author(s):  
Aylin Sendemir Urkmez ◽  
Sherrie G. Clark ◽  
Matthew B. Wheeler ◽  
Michael S. Goldwasser ◽  
Russell D. Jamison
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate

scholarly journals Biphasic calcium phosphate–casein bone graft fortified with Cassia occidentalis for bone tissue engineering and regeneration

2015 ◽  
Vol 38 (1) ◽  
pp. 259-266 ◽  
Author(s):  
B SANTHOSH KUMAR ◽  
T HEMALATHA ◽  
R DEEPACHITRA ◽  
R NARASIMHA RAGHAVAN ◽  
P PRABU ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Graft ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Cassia Occidentalis

Preparation of Porous Biphasic Calcium Phosphate-Gelatin Nanocomposite for Bone Tissue Engineering

2010 ◽  
Vol 11 ◽  
pp. 67-72 ◽  
Author(s):  
L. Bakhtiari ◽  
Hamid Reza Rezaie ◽  
S.M. Hosseinalipour ◽  
Mohammad A. Shokrgozar
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Porous Structure ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Bending Strength ◽  
Cell Viability Assay ◽  
Hydroxy Apatite ◽  
Porous Nanocomposite

A new porous structure as a bone tissue engineering scaffold was developed by a freeze-drying method. The porous nanocomposite was prepared from Biphasic Calcium Phosphate (BCP) which was a mixture of 70% hydroxy apatite and 30%ß-TCP (ß-Tricalcium Phosphate). Porogen was naphthalene and gelatin from bovine skin type B was used as polymer. Gelatin was stabilized with EDC (N-(3-dimethyl aminopropyl)-N´-ethyl carbodiimide hydrochloride) by a cross-linking method. The scaffold was characterized by scanning electronic microscope (SEM), Fourier-Transformed Infrared spectroscopy (FTIR). The biocompatibility of this nanocomposite carried out through MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole) cell viability assay. Also other properties of scaffold such as morphology, grain size, bending strength were investigated. Highly porous structure with interconnected porosities, good mechanical behavior and high biocompatibility with bone tissue, were benefits of this porous nanocomposite for bone tissue engineering.

scholarly journals Carbon nanotube reinforced polyvinyl alcohol/biphasic calcium phosphate scaffold for bone tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (67) ◽  
pp. 38998-39010 ◽  
Author(s):  
Weiwei Lan ◽  
Xiumei Zhang ◽  
Mengjie Xu ◽  
Liqin Zhao ◽  
Di Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Calcium Phosphate ◽  
Polyvinyl Alcohol ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Carbon ◽  
Biphasic Calcium Phosphate ◽  
Freeze Drying ◽  
Drying Method

In this paper, a well-developed porous carbon nanotube (CNT) reinforced polyvinyl alcohol/biphasic calcium phosphate (PVA/BCP) scaffold was fabricated by a freeze-thawing and freeze-drying method.

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