Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

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Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

Journal of Tissue Engineering ◽

10.1177/2041731420942734 ◽

2020 ◽

Vol 11 ◽

pp. 204173142094273

Author(s):

Karen M Marshall ◽

Janos M Kanczler ◽

Richard OC Oreffo

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Organotypic Culture ◽

Ex Vivo ◽

Chorioallantoic Membrane ◽

Experimental Methodology ◽

Chick Chorioallantoic Membrane ◽

Recent Developments ◽

Chorioallantoic Membrane Assay

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

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Preparation of microporous and macroporous biphasic calcium phosphate scaffolds modified via a biomimetic method for bone tissue engineering applications

Journal of Controlled Release ◽

10.1016/j.jconrel.2013.08.211 ◽

2013 ◽

Vol 172 (1) ◽

pp. e132

Author(s):

Lei Nie ◽

Qi Yang ◽

Jinping Suo

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biphasic Calcium Phosphate ◽

Engineering Applications ◽

Biomimetic Method

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Preparation and characterization of nano biphasic calcium phosphate/poly-L-lactide composite scaffold

Science and Engineering of Composite Materials ◽

10.1515/secm-2014-0100 ◽

2016 ◽

Vol 23 (1) ◽

pp. 37-44 ◽

Cited By ~ 1

Author(s):

Weizhong Yang ◽

Yong Yi ◽

Yuan Ma ◽

Li Zhang ◽

Jianwen Gu ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Cell Viability ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biphasic Calcium Phosphate ◽

Composite Scaffold ◽

Pore Wall ◽

Tissue Engineering Scaffold ◽

Plla Scaffold

AbstractNano biphasic calcium phosphate (BCP) particles were synthesized using the sol-gel method. As-prepared BCP particles were combined with poly-L-lactide (PLLA) to fabricate nano-BCP/PLLA composite scaffold through a series of processing steps containing solvent self-diffusion, hot-pressing, and particulate leaching. The composite had a suitable porous structure for bone tissue engineering scaffold. In comparison, micro-BCP/PLLA scaffold was studied as well. Nano-BCP particles were distributed homogeneously in the PLLA matrix, and much more tiny crystallites exposed on the surface of the pore wall. Due to the finer inorganic particle distribution in the PLLA phase and the larger area of the bioactive phase exposed in the pore wall surface, nano-BCP/PLLA scaffold had enhanced compressive strength, good bioactivity, and superior cell viability. A nonstoichiometric apatite layer could be rapidly formed on the surface of nano- BCP/PLLA when soaked in simulated body fluid. The MG-63 cell viability of nano-BCP/PLLA scaffold is significantly higher than that of micro-BCP/PLLA scaffold. Therefore, nano-BCP/PLLA composite may be a suitable alternative for bone tissue engineering scaffold.

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