Diphtheria toxoid and N -trimethyl chitosan layer-by-layer coated pH-sensitive microneedles induce potent immune responses upon dermal vaccination in mice

AbstractCapacitive field-effect electrolyte-diamond-insulator-semiconductor (EDIS) structures with O-terminated nanocrystalline diamond (NCD) as sensitive gate material have been realized and investigated for the detection of pH, penicillin concentration, and layer-by-layer adsorption of polyelectrolytes. The surface oxidizing procedure of NCD thin films as well as the seeding and NCD growth process on a Si-SiO2 substrate have been improved to provide high pH-sensitive, non-porous thin films without damage of the underlying SiO2 layer and with a high coverage of O-terminated sites. The NCD surface topography, roughness, and coverage of the surface groups have been characterized by SEM, AFM and XPS methods. The EDIS sensors with O-terminated NCD film treated in oxidizing boiling mixture for 45 min show a pH sensitivity of about 50 mV/pH. The pH-sensitive properties of the NCD have been used to develop an EDIS-based penicillin biosensor with high sensitivity (65-70 mV/decade in the concentration range of 0.25-2.5 mM penicillin G) and low detection limit (5 μM). The results of label-free electrical detection of layer-by-layer adsorption of charged polyelectrolytes are presented, too.

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Ultrathin Layer-by-Layer Hydrogels with Incorporated Gold Nanorods as pH-Sensitive Optical Materials

Chemistry of Materials ◽

10.1021/cm8023633 ◽

2008 ◽

Vol 20 (24) ◽

pp. 7474-7485 ◽

Cited By ~ 117

Author(s):

Veronika Kozlovskaya ◽

Eugenia Kharlampieva ◽

Bishnu P. Khanal ◽

Pramit Manna ◽

Eugene R. Zubarev ◽

...

Keyword(s):

Gold Nanorods ◽

Optical Materials ◽

Ph Sensitive ◽

Layer By Layer ◽

Ultrathin Layer

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Transcutaneous vaccination using a hydrogel patch induces effective immune responses to tetanus and diphtheria toxoid in hairless rat

Journal of Controlled Release ◽

10.1016/j.jconrel.2010.05.012 ◽

2011 ◽

Vol 149 (1) ◽

pp. 15-20 ◽

Cited By ~ 45

Author(s):

Kazuhiko Matsuo ◽

Yumiko Ishii ◽

Ying-Shu Quan ◽

Fumio Kamiyama ◽

Yohei Mukai ◽

...

Keyword(s):

Immune Responses ◽

Diphtheria Toxoid

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Layer-by-layer pH-sensitive nanoparticles for drug delivery and controlled release with improved therapeutic efficacy in vivo

Drug Delivery ◽

10.1080/10717544.2019.1709922 ◽

2020 ◽

Vol 27 (1) ◽

pp. 180-190 ◽

Cited By ~ 7

Author(s):

Wanfu Men ◽

Peiyao Zhu ◽

Siyuan Dong ◽

Wenke Liu ◽

Kun Zhou ◽

...

Keyword(s):

Drug Delivery ◽

Controlled Release ◽

Therapeutic Efficacy ◽

Ph Sensitive ◽

Layer By Layer

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Comparison of a Three-Component Acellular Pertussis Vaccine With a Whole-Cell Pertussis Vaccine in 15- Through 20-Month-Old Infants

PEDIATRICS ◽

10.1542/peds.93.4.656 ◽

1994 ◽

Vol 93 (4) ◽

pp. 656-659

Author(s):

Henry H. Bernstein ◽

Edward P. Rothstein ◽

Sarah S. Long ◽

Keith S. Reisinger ◽

Mark M. Blatter ◽

...

Keyword(s):

Immune Responses ◽

Pertussis Vaccine ◽

Tetanus Toxoid ◽

Geometric Mean ◽

Acellular Pertussis Vaccine ◽

Diphtheria Toxoid ◽

Enzyme Linked Immunosorbent Assay ◽

Double Blind ◽

Whole Cell ◽

Filamentous Hemagglutinin

Objective. To compare the immunogenicity and reactogenicity of a diphtheria and tetanus toxoids and three-component acellular pertussis vaccine (DTaP) with a diphtheria and tetanus toxoids and whole-cell pertussis vaccine (DTwP) when administered as a booster dose to infants 15 through 20 months of age. Design. Randomized, double-blind, comparative study. Setting. Three pediatric practices (two private; one hospital-based). Participants. One hundred and sixty-five healthy 15-through 20-month old infants. Selection procedures and interventions. Infants were randomly assigned in a 2:1 ratio to receive vaccine from a single lot of DTaP or from commercially available DTwP. DTaP contained 25 µg of pertussis toxoid, 25 µg of filamentous hemagglutinin, 8 µg of pertactin (69-kilodalton outer membrane protein), 25 flocculating units of diphtheria toxoid, and 10 flocculating units of tetanus toxoid per 0.5-mL dose. DTwP contained one half the concentrations of diphtheria and tetanus toxoids compared with DTaP and a pertussis component with a potency of 4 U/0.5-mL dose. Serum samples were obtained on the day of immunization and 4 weeks later. Adverse reactions were recorded by parents for 7 days after immunization. An interval history was obtained 4 weeks after immunization. Measurements and results. IgG antibody to pertussis toxoid, filamentous hemagglutinin, pertactin, diphtheria toxoid, and tetanus toxoid was measured by an indirect enzyme-linked immunosorbent assay (ELISA) method. One month after immunization, the geometric mean antibody levels after DTaP compared with DTwP were: pertussis toxoid, 70.6 vs 28 ELISA U/mL (P = .003); filamentous hemagglutinin, 183.4 vs 43 ELISA U/mL (P < .001); pertactin, 216 vs 49.9 ELISA U/mL (P < .001); diphtheria, 14.1 vs 14.9 IU/mL (P = .74); and tetanus, 11.9 vs 14.8 IU/mL (P = .089). After immunization with DTaP, most local and systemic adverse experiences were significantly fewer compared with DTwP (P < .05). Conclusions. This three-component DTaP vaccine demonstrates significantly greater immune responses to pertussis toxoid, filamentous hemagglutinin, and pertactin, equivalent immune responses to diphtheria and tetanus toxoids, and significantly less reactogenicity compared with a licensed DTwP.

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