Genetic characterization of respiratory syncytial viruses isolated from consecutive acute respiratory infections in a HIV infected child

2006 ◽  
Vol 35 (1) ◽  
pp. 41-45 ◽  
Author(s):  
J ARBIZA ◽  
M BEROIS ◽  
A DELFRARO ◽  
S FRABASILE ◽  
F MITOMA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Respiratory Infections ◽  
Infected Child ◽  
Acute Respiratory Infections ◽  
Respiratory Syncytial Viruses

scholarly journals Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
Sebastien Kenmoe ◽  
Marie-Astrid Vernet ◽  
Jerôme Le Goff ◽  
Véronique Beng Penlap ◽  
Astrid Vabret ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Respiratory Infections ◽  
Human Adenovirus ◽  
Acute Respiratory Infections

Spread of multidrug-resistant clonal complex 271 of serotype 19FStreptococcus pneumoniaein Beijing, China: characterization of serotype 19F

2013 ◽  
Vol 141 (12) ◽  
pp. 2492-2496 ◽  
Author(s):  
Q. H. LI ◽  
K. H. YAO ◽  
S. J. YU ◽  
X. MA ◽  
M. M. HE ◽  
...  
Keyword(s):  
Clonal Complex ◽  
Respiratory Infections ◽  
Multidrug Resistant ◽  
Acute Respiratory Infections ◽  
Resistant Clone ◽  
Resistant Strains ◽  
Beijing China ◽  
Susceptibility Rate

SUMMARYWe investigated the genetic structure of 120 isolates of serotype 19FStreptococcus pneumoniaefrom Chinese children with acute respiratory infections collected from 1997 to 2006, and 2010. All strains were susceptible to vancomycin and levofloxacin, and only five strains were not susceptible to penicillin. The non-susceptibility rate to cephalosporins increased from 1997 to 2010. Of 119 erythromycin-resistant strains, 60 carried bothermBandmefAgenes. The percentage of clonal complex 271 (CC271) increased from 14·3% in 1997–1998 to 92% in 2010, whereas that of CC983 decreased from 64·3% to 0%. CC271 had a higher non-susceptibility rate toβ-lactam antibiotics than CC983 and other CCs. The increased non-susceptibility rate toβ-lactam antibiotics in serotype 19F pneumococci was found to be associated with the spread of the international resistant clone CC271 presumably caused by antibiotic pressure. Long-term surveys of serotype 19FS. pneumoniaeare required to monitor CC prevalence and trends in antimicrobial resistance.

scholarly journals Detection and Differentiation of SARS-CoV-2, Influenza, and Respiratory Syncytial Viruses by CRISPR

Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 823
Author(s):  
Huifen Zhou ◽  
Jen-Hui Tsou ◽  
Molangur Chinthalapally ◽  
Hongjie Liu ◽  
Feng Jiang
Keyword(s):  
Early Detection ◽  
Influenza A ◽  
Respiratory Infections ◽  
Limit Of Detection ◽  
Respiratory Viruses ◽  
Cross Reaction ◽  
Sensitive Detection ◽  
Acute Respiratory Infections ◽  
Prevention And Treatment ◽  
Respiratory Syncytial Viruses

SARS-CoV-2, influenza, and respiratory syncytial viruses (RSVs) cause acute respiratory infections with similar symptoms. Since the treatments and outcomes of these infections are different, the early detection and accurate differentiation of the viruses are clinically important for the prevention and treatment of the diseases. We previously demonstrated that clustered regularly interspaced short palindromic repeats (CRISPR) could rapidly and precisely detect SARS-CoV-2. The objective of this study was to develop CRISPR as a test for simultaneously detecting and accurately distinguishing the viruses. The CRISPR assay with an RNA guide against each virus was performed in the reference standards of SARS-CoV-2, influenza A and B, and RSV. The CRISPR assay had a limit of detection of 1–100 copies/µL for specifically detecting SARS-CoV-2, influenza A and B, and RSV without cross-reaction with other respiratory viruses. The validation of the test in nasopharyngeal specimens showed that it had a 90–100% sensitivity and 100% specificity for the detection of SARS-CoV-2, influenza A and B, and RSV. The CRISPR assay could potentially be used for sensitive detection and specific differentiation of the respiratory viruses.

scholarly journals Identification and Characterization of a New Orthoreovirus from Patients with Acute Respiratory Infections

PLoS ONE ◽  
2008 ◽  
Vol 3 (11) ◽  
pp. e3803 ◽  
Author(s):  
Kaw Bing Chua ◽  
Kenny Voon ◽  
Gary Crameri ◽  
Hui Siu Tan ◽  
Juliana Rosli ◽  
...  
Keyword(s):  
Respiratory Infections ◽  
Acute Respiratory Infections ◽  
Identification And Characterization

scholarly journals Characterization of novel canine bocaviruses and their association with respiratory disease

2012 ◽  
Vol 93 (2) ◽  
pp. 341-346 ◽  
Author(s):  
Amit Kapoor ◽  
Natasha Mehta ◽  
Edward J. Dubovi ◽  
Peter Simmonds ◽  
Lakshmanan Govindasamy ◽  
...  
Keyword(s):  
Respiratory Diseases ◽  
Genetic Characterization ◽  
Respiratory Infections ◽  
Novel Species ◽  
Association Studies ◽  
Evolutionary Analysis ◽  
Vp2 Gene ◽  
Minute Virus ◽  
Comparative Genetic Analysis

We report the first identification, genetic characterization and disease association studies of several novel species of canine bocaviruses (CBoV). Evolutionary analysis confirmed that CBoV are genetically distinct from the only other known canine bocavirus, minute virus of canines, with which they share less than 63, 62 and 64 % protein identity in NS, NP and VP genes, respectively. Comparative genetic analysis of 37 VP gene variants found in diseased and healthy animals showed that these novel viruses are genetically highly diverse and are common in canine respiratory infections that have remained undetected until now. Interestingly, we observed that a CBoV genotype with a unique deletion in the VP2 gene was significantly more prevalent in animals with respiratory diseases compared with healthy animals.

scholarly journals ENTEROVIRUSES ISOLATED FROM PATIENTS WITH ACUTE RESPIRATORY INFECTIONS DURING SEVEN YEARS IN RIO DE JANEIRO (1985-1991)

1998 ◽  
Vol 40 (6) ◽  
pp. 337-342 ◽  
Author(s):  
Silvana A.R. PORTES ◽  
Edson E. DA SILVA ◽  
Marilda M. SIQUEIRA ◽  
Ana Maria B. DE FILIPPIS ◽  
Murilo M. KRAWCZUK ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Respiratory Infections ◽  
Acute Respiratory Infections ◽  
Upper Respiratory Tract ◽  
Coxsackievirus B4 ◽  
Respiratory Syncytial Viruses ◽  
Tract Infections ◽  
Virus Isolates

Enteroviruses were investigated in respiratory secretions collected from patients with acute respiratory infections (ARI) over a seven year period (1985-1991), as part of a longitudinal study of ARI aetiology. All the viruses that are most commonly associated with ARI were found in this study. Among the virus isolates, enteroviruses were only less frequent than respiratory syncytial viruses, adenoviruses and influenzaviruses. Forty five enterovirus samples were isolated from patients with either upper respiratory tract infections (URTI) or lower respiratory tract infections (LRTI). From these enterovirus isolates, thirty one samples were identified as poliovirus (n=18) and non polio enterovirus (n=13) by serum neutralization. Poliovirus were identified as type 1 and 2 and all of them were vaccinal strains. From thirteen non polio enterovirus, twelve were identified as echovirus serotypes 1, 2, 7, 11, 19 and 31. The remainder was identified as coxsackievirus B4.

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