Monitoring of human cytomegalovirus infection in immunosuppressed patients using real-time PCR on whole blood

2007 ◽  
Vol 40 (3) ◽  
pp. 173-179 ◽  
Author(s):  
C. Deback ◽  
A.M. Fillet ◽  
N. Dhedin ◽  
B. Barrou ◽  
S. Varnous ◽  
...  
Keyword(s):  
Real Time ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Cytomegalovirus Infection ◽  
Immunosuppressed Patients ◽  
Human Cytomegalovirus Infection

scholarly journals Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real-Time PCR Assay

2003 ◽  
Vol 41 (8) ◽  
pp. 3840-3845 ◽  
Author(s):  
C. Mengelle ◽  
K. Sandres-Saune ◽  
C. Pasquier ◽  
L. Rostaing ◽  
J.-M. Mansuy ◽  
...  
Keyword(s):  
Real Time ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Automated Extraction ◽  
Pcr Assay

scholarly journals The Prevalence of Human Cytomegalovirus Infection in Children Leukemia by Chip Digital PCR

2021 ◽  
Author(s):  
Wen-Jun Wang ◽  
Miao Feng ◽  
Feng He ◽  
Juan Song ◽  
Qin-Qin Song ◽  
...  
Keyword(s):  
Viral Load ◽  
Human Cytomegalovirus ◽  
Whole Blood ◽  
Cytomegalovirus Infection ◽  
Hcmv Infection ◽  
Laboratory Diagnosis ◽  
Digital Pcr ◽  
Clinical Treatment ◽  
Diagnosis Rate ◽  
Human Cytomegalovirus Infection

Abstract Background To establish a method for detecting HCMV viral load to guide clinical treatment by chip digital PCR (cdPCR). Methods 5.67×106TCID50/ml of HCMV AD169 was serially diluted to evaluate sensitive of cdPCR, HSV-1, HSV-2, VZV, EBV, HHV-6 and HHV-7 were used to evaluate the specificity of HCMV cdPCR. HCMV infection were analyzed in 110 children leukemia whole blood by RT-qPCR and cdPCR. Results The sensitive of HCMV cdPCR was up to 71 ± 32 copies/ml, which is higher than that of RT-qPCR. HCMV cdPCR did not cross react with other herpesviruses. The cdPCR effectively detected 7 HCMV positive samples, making the laboratory diagnosis rate of HCMV increased by 6.36% (7/110) for children leukemia patients. And the prevalence of HCMV infection increased from 28.18–34.54% in 110 children leukemia patients by cdPCR. Conclusion cdPCR is more sensitive to detect viral load than RT-qPCR. The cdPCR may be used to evaluate relationship between viral load and progression of HCMV infection in patients.

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