Performance evaluation of the FDA-approved Determine™ HIV-1/2 Ag/Ab Combo assay using plasma and whole blood specimens

Summary: The capillus HIV-1/HIV-2 latex agglutination (LA) test was evaluated for its potential as an initial screening test in primary health care. For the serum study, panels totalling 289 HIV-positive sera and 323 known HIV-negative sera plus 50 individual seroconversion samples were tested by capillus. Paired blood specimens were also collected in heparinized and plain tubes from 501 consecutive patients with newly diagnosed sexually transmitted diseases (STDs) attending an STD clinic at a Transvaal hospital. Overall, an initial sensitivity of 99.3% and an initial specificity of 99.7% were obtained by visual reading of the capillus HIV-1/ HIV-2 LA tests on serum samples. Capillus also detected 40 (80%) of the 50 seroconversion samples. Of the 501 paired plain and heparinized blood specimens, serum testing by enzyme immunoassay (EIA) and indirect immunofluorescence/ Western blot (IFA/WB) showed 147 (29%) to be HIV Ab positive. Capillus testing of the paired specimens correctly identified all 147 known positive patients and 345 of the 346 negative patients, thus showing an initial sensitivity of 100% and an initial specificity of 99.7% for the testing of heparinized whole blood by a relatively unskilled health worker. It was concluded that the capillus HIV-1/HIV-2 LA test would be suitable for use as a primary screening test in small outlying laboratories or primary health care clinics.

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Performance evaluation of a point-of-care whole blood viral load test (SAMBA II HIV-1 Semi-Q Whole Blood) to optimise HIV treatment

http://isrctn.com/ ◽

10.1186/isrctn12803987 ◽

2016 ◽

Author(s):

Sarah Oakley-Mudge

Keyword(s):

Performance Evaluation ◽

Viral Load ◽

Whole Blood ◽

Point Of Care ◽

Hiv Treatment ◽

Load Test ◽

Viral Load Test ◽

Hiv 1 ◽

Blood Viral Load

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Performance evaluation of a whole blood propofol analyser

Journal of Clinical Monitoring and Computing ◽

10.1007/s10877-011-9330-0 ◽

2012 ◽

Vol 26 (1) ◽

pp. 29-36 ◽

Cited By ~ 15

Author(s):

Bo Liu ◽

David M. Pettigrew ◽

Stephen Bates ◽

Peter G. Laitenberger ◽

Gavin Troughton

Keyword(s):

Performance Evaluation ◽

Whole Blood

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Controlled storage conditions prolong stability of biochemical components in whole blood

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2005.036 ◽

2005 ◽

Vol 43 (2) ◽

Cited By ~ 8

Author(s):

Marta Stahl ◽

Ivan Brandslund

Keyword(s):

Whole Blood ◽

Storage Conditions ◽

Ion Concentration ◽

Potassium Ions ◽

Uncertainty Of Measurements ◽

Biochemical Components ◽

Turn Around Time ◽

Analytical Result ◽

Blood Specimens ◽

Doctor's Office

AbstractBlood specimens from primary care centres are normally transported to central laboratories by mail. This necessitates centrifugation and separation, especially since the potassium ion concentration in whole blood changes during storage at ambient temperature. Thus, because of the growing awareness of and concern for pre-analytical contributions to the uncertainty of measurements, we investigated 27 components and their stability under controlled temperature conditions from 17 to 23°C. We found that storage of whole blood can be prolonged by up to 8–12h for all components examined, including potassium ions, when stored at 20±0.2°C. We conclude that this opens the possibility for establishing a pick-up service, by which whole blood specimens stored at 20–21°C can be collected at the doctor's office, making centrifugation, separation and mailing superfluous. In addition, the turn-around time from sample drawing to reporting the analytical result would be shortened. After investments in thermostatted boxes and logistics, the system could reduce costs for transporting blood samples from general practice centres to central laboratories.

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Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood

Annals of Laboratory Medicine ◽

10.3343/alm.2016.36.6.603 ◽

2016 ◽

Vol 36 (6) ◽

pp. 603-606 ◽

Cited By ~ 4

Author(s):

Jong Eun Park ◽

Ji-Youn Kim ◽

Sun Ae Yun ◽

Myoung-Keun Lee ◽

Hee Jae Huh ◽

...

Keyword(s):

Performance Evaluation ◽

Real Time ◽

Real Time Pcr ◽

Whole Blood ◽

The Real

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Occurrence of adenovirus infection and clinical impact in paediatric stem cell transplant recipients

Microbiologia Medica ◽

10.4081/mm.2016.5850 ◽

2016 ◽

Vol 31 (3) ◽

Author(s):

Gabriele Bianco ◽

Cristina Costa ◽

Andrea Piceghello ◽

Francesca Sidoti ◽

Mareva Giacchino ◽

...

Keyword(s):

Stem Cell ◽

Whole Blood ◽

Limit Of Detection ◽

Antiviral Agent ◽

Clinical Impact ◽

Cell Transplant ◽

Adenovirus Infection ◽

Hematopoietic Stem ◽

Organ Specific ◽

Blood Specimens

In this study, the occurrence and clinical impact of adenovirus (AdV) infection was investigated in paediatric hematopoietic stem cell transplantation (HSCT) recipients. A number of 603 specimens (including whole blood, respiratory and other samples) from 181 patients were tested by real-time polymerase chain reaction; clinical outcome was investigated. Overall, 118/603 (19.6%) specimens from 21/181 (11.6%) patients resulted positive to AdV (including 17.3, 29.9, 17.6, and 15.8% of total number of whole blood, respiratory, urine and other specimens, respectively). On whole blood specimens, viral loads ranged from <600 (limit of detection) to >5×10<sup>6</sup> copies/mL, with a median value 2×104. Multiple specimens were positive in patients in which viral load on whole blood was high. Adenoviral positivity on whole blood was associated to poor prognosis, as death occurred in three of ten (30%) patients with persistent positivity on whole blood specimens, also despite the administration of an antiviral agent (cidofovir). Adenovirus infection can account for systemic and/or organ-specific signs/symptoms in approximately 10% of paediatric HSCT recipients. At moment, there is no indication for routine monitor of AdV in these patients, although AdV aetiology of infectious transplant complications should be taken in account.

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Use of the Procleix HIV‐1 and HCV discriminatory assays to detect HIV and HCV RNA in whole blood

Transfusion ◽

10.1046/j.1537-2995.2002.00266.x ◽

2002 ◽

Vol 42 (11) ◽

pp. 1525-1527 ◽

Cited By ~ 5

Author(s):

L.H. Tobler ◽

Janel Dockter ◽

S.L. Stramer ◽

S.H. Kleinman ◽

D. Todd ◽

...

Keyword(s):

Whole Blood ◽

Hcv Rna ◽

Hiv 1

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Detection of Pneumocystis carinii DNA in Blood Specimens from Human Immunodeficiency Virus-Infected Patients by Nested PCR

Journal of Clinical Microbiology ◽

10.1128/jcm.37.1.127-131.1999 ◽

1999 ◽

Vol 37 (1) ◽

pp. 127-131 ◽

Cited By ~ 21

Author(s):

Meja Rabodonirina ◽

Laurent Cotte ◽

André Boibieux ◽

Karine Kaiser ◽

Martine Mayençon ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Whole Blood ◽

Nested Pcr ◽

Large Subunit ◽

Pneumocystis Carinii ◽

Proteinase K ◽

Rrna Gene ◽

Immunodeficiency Virus ◽

Blood Specimens ◽

Large Subunit Rrna

The detection of Pneumocystis carinii DNA in blood by PCR could be useful for studying the natural history of pneumocystosis and could also be a noninvasive diagnostic method. The results of previous studies are nevertheless conflicting. In our study, we compared three commercially available DNA extraction kits (GeneReleaser, QIAamp Tissue Kit, and ReadyAmp Genomic DNA Purification System) and proteinase K and proteinase K-phenol-chloroform treatments for the extraction of P. carinii DNA from dilutions of a P. carinii f. sp.hominis cyst suspension mixed with human whole blood. A rapid and simple nested PCR protocol which amplifies a portion of the mitochondrial large-subunit rRNA gene was applied to all the extraction products. The QIAmp Tissue Kit was the most effective kit for the isolation of amplification-ready P. carinii DNA and was used with nested PCR for the testing of whole-blood specimens from 35 immunocompetent control patients and 84 human immunodeficiency virus (HIV)-infected patients investigated for pulmonary disease and/or fever. In HIV-infected patients, P. carinii DNA was detected by nested PCR in blood samples from 3 of 14 patients with microscopically proven P. carinii pneumonia, 7 of 22 patients who were considered to be colonized with P. carinii, and 9 of 48 patients who were neither infected nor colonized with P. carinii. P. carinii DNA was not detected in blood specimens from the 35 immunocompetent patients. P. carinii DNA in blood might represent viable P. carinii organisms or DNA complexes released from pulmonary phagocytes. In conclusion, P. carinii DNA may be detected in whole blood from HIV-infected patients, but the nature and the meaning of the circulating form of P. carinii remain to be established.

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