Characterization of neural cell differentiation potential of human amnion derived epithelia cells and mesenchymal stem cells

The mesenchymal stem cells have multidirectional differentiation potential and can differentiate into adipocytes, osteoblasts, cartilage tissue, muscle cells and so on. The adipogenic differentiation of mesenchymal stem cells is of great significance for the construction of tissue-engineered fat and the treatment of soft tissue defects. Exosomes are nanoscale vesicles secreted by cells and widely exist in body fluids. They are mainly involved in cell communication processes and transferring cargo contents to recipient cells. In addition, exosomes can also promote tissue and organ regeneration. Recent studies have shown that various exosomes can influence the adipogenic differentiation of stem cells. In this review, the effects of exosomes on stem cell differentiation, especially on adipogenic differentiation, will be discussed, and the mechanisms and conclusions will be drawn. The main purpose of studying the role of these exosomes is to understand more comprehensively the influencing factors existing in the process of stem cell differentiation into adipocytes and provide a new idea in adipose tissue engineering research.

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ID: 1054 Comparison between fresh and cryopreserved Human Amnion Mesenchymal Stem Cells (HAMCs) in terms of serial passaging, morphology and differentiation potential during long term culture

Biomedical Research and Therapy ◽

10.15419/bmrat.v4is.329 ◽

2017 ◽

Vol 4 (S) ◽

pp. 134

Author(s):

Sandra Lisa Gumpil ◽

Kamaruzaman Ampon ◽

Helen Lasimbang ◽

Simat Siti Fatimah ◽

S.Vijay Kumar

Keyword(s):

Stem Cells ◽

Cell Culture ◽

Mesenchymal Stem Cells ◽

Cellular Therapy ◽

Enzymatic Digestion ◽

Positive Staining ◽

Differentiation Potential ◽

Human Amnion ◽

Long Term Culture

The therapeutic efficiency of Human Amnion Mesenchymal stem cells (HAMCs) is significantly promising. However, its sustainability in long term sub-cultivation has yet to be identified, especially post-cryopreservation. There are concerns whether stem cells which are cryopreserved will be able to retain its functions and differentiation potential efficiently. The aim of this study is to determine the effects of cryopreservation on the serial passaging, morphology and differentiation potential of HAMCs culture post-cryopreservation compared to fresh HAMCs culture. HAMCs was isolated through serial enzymatic digestion of the amnion membrane of human term placenta after delivery. Fresh HAMCs cultures were sub-cultivated until passage 15 while cryopreserved HAMCs samples was preserved at passage 2 of the cell culture and revived after 3 months of cryopreservation period. Through microscopic observation, the cryopreserved HAMCs started to flatten and become larger in size, losing it fibroblastic feature as early as passage 6. The enlarged and flatten morphological features of the HAMCs indicate that the cells began to lose its proliferative activity at this point. In comparison, the HAMCs normally began to enlarge when reaching passage 15 in non-cryopreserved HAMCs cultures. Fresh HAMCs cultures were able to be sustained up to 15 passages while cryopreserved HAMCs were not able to survive pass 10 passages of cell culture. Furthermore, it was observed that both fresh and cryopreserved HAMCs were able to retain its differentiation potential through osteogenesis and adipogenesis medium induction. This was experimentally visualized through positive staining of the calcium composite and lipid droplet in the induced HAMCs culture using Von Kossa stain solution and Oil Red-O stain solution, respectively. Despite retaining it differentiation potential, cryopreserved HAMCs were only able to survive a few passages after being revived. The result suggests that fresh HAMCs is a more suitable candidate to be used in cellular therapy and various clinical application as it were able to retain all its function in long term culture.

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Differential expression profiles of lncRNAs and mRNAs during male germ-like cell differentiation in human umbilical mesenchymal stem cells by RNA-seq

10.21203/rs.3.rs-281787/v1 ◽

2021 ◽

Author(s):

Lichun Xie ◽

Guichi Zhou ◽

Lian Ma ◽

Feiqiu Wen

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cell Differentiation ◽

Expression Profiles ◽

Differentiation Potential ◽

Non Coding Rna ◽

Development And Differentiation ◽

Non Coding Rnas ◽

Minimum Numbers ◽

Umbilical Mesenchymal Stem Cells

Abstract Background: Long non-coding RNAs (lncRNAs) are key regulators of various biological processes and crucial for cell development and differentiation. However, their roles in the differentiation of human umbilical mesenchymal stem cells (HUMSCs) into male germ-like cells remain largely unknown. Method: Here, the expression of lncRNAs and mRNAs in undifferentiated HUMSCs and HUMSCs undergoing differentiation into male germ-like cells was analyzed. RNA-sequencing was performed to profile the expression of non-coding RNAs. We analyzed the total expression of lncRNAs/mRNAs at three time points during HUMSC differentiation [day (D)7, D14, and D21]. Result: Expression profiling revealed 110 lncRNAs, 584 mRNAs, and 21 miRNAs common to the three experimental groups during HUMSC male germ-like cell differentiation. The maximum and minimum total overall lncRNA expression occurred on D14 (638) and D21 (283), respectively. The maximum and minimum numbers of up-regulated mRNAs were observed on D21 (2,398) and D7 (2,106), respectively. The maximum and minimum numbers of down-regulated mRNAs were observed on D14 (3,357) and D21 (202), respectively. The expression level of mRNA ENST00000486554 was up-regulated on D7, D14, and D21 after induction. Pathway analysis identified meiotic signaling pathways and nitrogen metabolism as being associated with the differentiation potential of HUMSC male germ-like cells. Non-coding RNA expression profiles significantly differed in HUMSC male germ-like cell differentiation. One mRNA, ENST00000486554, was crucial for differentiation. Conclusions: Our results provide a systematic perspective on the potential functions of non-coding RNAs and novel insights into the complicated regulatory mechanisms underlying the differentiation of HUMSCs into male germ-like cells.

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