Effect of different irradiation doses on human and murine fibroblasts used as feeder layer for keratinocytes’ culture

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S181-S182
Author(s):  
A. Sierra-Sanchez ◽  
A. Ordóñez-Luque ◽  
A. Ruiz-Garcia ◽  
J. Guerrero ◽  
N. Fernández-Porcel ◽  
...  
Keyword(s):  
Feeder Layer ◽  
Murine Fibroblasts

Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate

2010 ◽  
Vol 191 (1) ◽  
pp. 21-35 ◽  
Author(s):  
Laura Panacchia ◽  
Elena Dellambra ◽  
Sergio Bondanza ◽  
Patrizia Paterna ◽  
Riccardo Maurelli ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
Feeder Layer ◽  
Murine Fibroblasts

scholarly journals A Study on the Effects of 50 Hz Magnetic Fields on UV-induced Radical Reactions in Murine Fibroblasts

2010 ◽  
Vol 51 (5) ◽  
pp. 609-613 ◽  
Author(s):  
Ari MARKKANEN ◽  
Jonne NAARALA ◽  
Jukka JUUTILAINEN
Keyword(s):  
Magnetic Fields ◽  
Radical Reactions ◽  
Murine Fibroblasts

Stromal cell and human prostatic epithelial cell in-vitro co-coltures: Growth and morphology

1996 ◽  
Vol 63 (1_suppl) ◽  
pp. 65-68
Author(s):  
S. De Angeli ◽  
A. Fandella ◽  
C. Gatto ◽  
S. Buoro ◽  
C. Favretti ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Marked Reduction ◽  
Stromal Cell ◽  
Optical Microscope ◽  
Neutral Red ◽  
Cellular Growth ◽  
Murine Fibroblasts ◽  
Prostatic Epithelial Cell

A study was carried out on the effect of stroma-epithelium interaction on cellular growth and morphology in co-coltures of U285 prostatic epithelial cells with human prostatic and esophageal stromal cells and with murine fibroblasts of the 3T3-J2 line. The proliferation rate was determined by growth tests of neutral red and kenacid blue. Morphological observations were made under optical microscope on the same cultures used for the growth tests. Results highlighted a marked reduction in cellular growth in the co-cultures compared to control cultures, as well as the tendency of the stromal and epithelial cells to re-organise themselves in pseudo-acinous structures.

scholarly journals Tyrosine Phosphorylation of Selected Secretory Carrier Membrane Proteins, SCAMP1 and SCAMP3, and Association with the EGF Receptor

1998 ◽  
Vol 9 (7) ◽  
pp. 1661-1674 ◽  
Author(s):  
Theodore T. Wu ◽  
J. David Castle
Keyword(s):  
Membrane Proteins ◽  
Egf Receptor ◽  
Tyrosine Phosphatase ◽  
Chinese Hamster ◽  
Tyrosine Residue ◽  
Cell Interior ◽  
Murine Fibroblasts ◽  
Secretory Carrier Membrane Proteins

Secretory carrier membrane proteins (SCAMPs) are ubiquitously expressed proteins of post-Golgi vesicles. In the presence of the tyrosine phosphatase inhibitor vanadate, or after overexpression in Chinese hamster ovary (CHO) cells, SCAMP1 and SCAMP3 are phosphorylated selectively on tyrosine residue(s). Phosphorylation is reversible after vanadate washout in situ or when isolated SCAMP3 is incubated with the recombinant tyrosine phosphatase PTP1B. Vanadate also causes the partial accumulation of SCAMP3, but not SCAMP1, in “patches” at or near the cell surface. A search for SCAMP kinase activities has shown that SCAMPs 1 and 3, but not SCAMP2, are tyrosine phosphorylated in EGF-stimulated murine fibroblasts overexpressing the EGF receptor (EGFR). EGF catalyzes the progressive phosphorylation of the SCAMPs up to 1 h poststimulation and may enhance colocalization of the EGFR and SCAMP3 within the cell interior. EGF also induces SCAMP–EGFR association, as detected by coimmunoprecipitation, and phosphorylation of SCAMP3 is stimulated by the EGFR in vitro. These results suggest that phosphorylation of SCAMPs, either directly or indirectly, may be functionally linked to the internalization/down-regulation of the EGFR.

Elimination of feeder layer effects in cultured keratinocyte grafts

2000 ◽  
Vol 191 (4) ◽  
pp. S22 ◽  
Author(s):  
Wesley G Schooler ◽  
Kevin J Bruen ◽  
Suzan deSerres ◽  
Bruce A Cairns ◽  
Jeffrey A Frelinger ◽  
...  
Keyword(s):  
Feeder Layer
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