Gastroprotective effect of α-terpineol-loaded polymethyl methacrylate particles on gastric injury model

Background. Magnolia officinalis Rehd. and Wils. is widely used in Asian countries because of its multiple pharmacological effects. This study investigated the gastroprotective effect and mechanisms of the ethanol extracts from the bark of Magnolia officinalis (MOE) against ethanol-induced gastric mucosal damage in rats. Methods. MOE was prepared by reflux extraction with 70% ethanol, and its main compounds were analyzed by UPLC-Q-Exactive Orbitrap-MS. DPPH, ABTS, and FRAP methods were used to evaluate the antioxidant capacity of MOE in vitro. The gastroprotective effects of MOE were evaluated by the area of gastric injury, H&E (hematoxylin-eosin), and PAS (periodic acid-Schiff). The mechanism was explored by measuring the levels of cytokines and protein in the NF-κB signaling pathway. Results. 30 compounds were identified from MOE, mainly including lignans and alkaloids. MOE presented a high antioxidant activity in several oxidant in vitro systems. Gastric ulcer index and histological examination showed that MOE reduced ethanol-induced gastric mucosal injury in a dose-dependent manner. MOE pretreatment significantly restored the depleted activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) enzymes, reduced malondialdehyde (MDA), and prostaglandin E2 (PGE2) levels in the gastric tissue in rats. In addition, MOE also inhibited the activation of nuclear factor kappa B (NF-κB) pathway and decreased the production of proinflammatory cytokines. Conclusions. The gastroprotective effect of MOE was attributed to the inhibition of oxidative stress and the NF-κB inflammatory pathway. The results provided substantial evidence that MOE could be a promising phytomedicine for gastric ulcer prevention.

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Gastroprotective effect of crocin in ethanol-induced gastric injury in rats

Chemico-Biological Interactions ◽

10.1016/j.cbi.2015.01.015 ◽

2015 ◽

Vol 229 ◽

pp. 26-35 ◽

Cited By ~ 66

Author(s):

Shohda A. El-Maraghy ◽

Sherine M. Rizk ◽

Nancy N. Shahin

Keyword(s):

Gastric Injury ◽

Gastroprotective Effect

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Antioxidative and Anti-Inflammatory Effects of Water Extract of Acrostichum aureum Linn. against Ethanol-Induced Gastric Ulcer in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/3585394 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Xue Wu ◽

Qionghui Huang ◽

Nan Xu ◽

Jian Cai ◽

Dandan Luo ◽

...

Keyword(s):

Gastric Ulcer ◽

Biological Effects ◽

Interleukin 1 ◽

Water Extract ◽

Gastric Injury ◽

Mangrove Forests ◽

Inflammatory Infiltration ◽

Injury Model ◽

Protein Expressions ◽

Necrosis Factor

Acrostichum aureum Linn., a medicinal pteridophyte growing in mangrove forests and coastal regions of tropical and subtropical areas worldwide, has been proved to possess various biological effects. However, the protective effect of Acrostichum aureum Linn. against gastric ulcer still remains unidentified. Therefore, the gastroprotective effect of the water extract of Acrostichum aureum Linn. (WEAC) was investigated in ethanol-induced gastric injury model. According to our results, pretreatment with WEAC (100, 200, and 400 mg/kg) could dramatically decrease the ulcer areas and ameliorate the pathological damage induced by alcohol in rat’s gastric tissues. In addition, WEAC administration prevented the stomach from oxidative damage via markedly increasing the levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and decreasing the malondialdehyde (MDA). Besides, WEAC pretreatment alleviated inflammatory infiltration by reducing the secretion of proinflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) as well as decreasing the protein expressions of phosphorylation of IκBα and p65. Taken together, WEAC exerted potential therapeutic efficacy for gastric ulceration, and this may be involved in the suppression of oxidative stress and inflammatory response.

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Glucocorticoids Are Involved in the Gastroprotective Effect of Forced Treadmill Running on Indomethacin–induced Gastric Injury in Rats

The FASEB Journal ◽

10.1096/fasebj.2021.35.s1.00322 ◽

2021 ◽

Vol 35 (S1) ◽

Author(s):

Ludmila Filaretova ◽

Olga Komkova ◽

Natalia Yarushkina

Keyword(s):

Treadmill Running ◽

Gastric Injury ◽

Gastroprotective Effect

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Augmenting Effect of DA-9601 on Ghrelin in an Acute Gastric Injury Model

Gut and Liver ◽

10.5009/gnl.2011.5.1.52 ◽

2011 ◽

Vol 5 (1) ◽

pp. 52-56 ◽

Cited By ~ 6

Author(s):

Yoo Hum Baek ◽

Kang Nyeong Lee ◽

Dae Won Jun ◽

Byung Chul Yoon ◽

Ju Mi Kim ◽

...

Keyword(s):

Gastric Injury ◽

Injury Model

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Royal jelly attenuates gastric mucosal injury in a rat ethanol-induced gastric injury model

Molecular Biology Reports ◽

10.1007/s11033-020-05939-w ◽

2020 ◽

Vol 47 (11) ◽

pp. 8867-8879

Author(s):

Yasin Duran ◽

İhsan Karaboğa ◽

Fatin Rüştü Polat ◽

Elif Polat ◽

Zeynep Fidanol Erboğa ◽

...

Keyword(s):

Royal Jelly ◽

Mucosal Injury ◽

Gastric Mucosal Injury ◽

Gastric Injury ◽

Injury Model

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The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut (Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats

Nutrients ◽

10.3390/nu12041138 ◽

2020 ◽

Vol 12 (4) ◽

pp. 1138 ◽

Cited By ~ 2

Author(s):

Rui Liu ◽

Yun-Tao Hao ◽

Na Zhu ◽

Xin-Ran Liu ◽

Jia-Wei Kang ◽

...

Keyword(s):

Body Weight ◽

Gastric Content ◽

Juglans Regia ◽

Antioxidant Properties ◽

Mucosal Injury ◽

Gastric Mucosal Injury ◽

Gastric Injury ◽

Protective Effects ◽

Sprague Dawley ◽

Gastroprotective Effect

The study investigated the protective effect of walnut oligopeptides (WOPs) against ethanol-induced gastric injury using Sprague-Dawley (SD) rats. Rats were randomly divided into seven groups based on body weight (10/group), normal group, ethanol group, whey protein group (220 mg/kg body weight), omeprazole group (20 mg/kg body weight), and three WOPs groups (220, 440, 880 mg/kg body weight). After 30 days of treatment with WOPs, rats were given 5 mL/kg absolute ethanol by gavage to induce gastric mucosal injury. Gastric ulcer index (GUI) were determined and the following measured; gastric content pH, gastric mucin, endogenous pepsinogens (PG), prostaglandin E2 (PGE2), inflammatory cytokines, oxidative stress indicators, and the expression of apoptosis-related proteins were measured to evaluate the gastroprotective effect of WOPs. The results showed that the administration with WOPs markedly mitigated the hemorrhagic gastric lesions caused by ethanol in rats, and decreased the GUI, the gastric content pH, PG1, PG2, and NO levels, enhanced mucin and PGE2. Also, WOPs repressed gastric inflammation through the reduction of TNF-α, IL-6, IL-1β and increase IL-10 levels, and revealed antioxidant properties with the enhancement of superoxide dismutase, glutathione, and catalase activity, while reduction of malondialdehyde. Moreover, WOPs treatment significantly down-regulated Bax, caspase-3 and nuclear factor-κB p65 (NF-κB p65) expression, while up-regulating the expression of Bcl-2 and inhibitor kappa Bα (IκBα) protein. These results indicated that WOPs have protective effects against ethanol-induced gastric mucosal injury in rats through anti-inflammatory, anti-oxidation, and anti-apoptosis mechanisms.

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