Activated sludge microbial communities influencing the performance of a full-scale wastewater treatment plant treating optoelectronic wastewater

2021 ◽  
pp. 106833
Author(s):  
Jingsi Gao ◽  
Nan Yang ◽  
Hehuan Liao ◽  
Hao Yuan ◽  
Jia Zhu
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale

Evaluating the treatment performance of a full scale Activated Sludge Plant in Islamabad, Pakistan

2012 ◽  
Vol 7 (1) ◽  
Author(s):  
S. S. Fatima ◽  
S. Jamal Khan
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Suspended Solids ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Full Scale ◽  
Operational Parameter ◽  
Mixed Liquor ◽  
Treatment Performance

In this study, the performance of wastewater treatment plant located at sector I-9 Islamabad, Pakistan, was evaluated. This full scale domestic wastewater treatment plant is based on conventional activated sludge process. The parameters which were monitored regularly included total suspended solids (TSS), mixed liquor suspended solids (MLSS), mixed liquor volatile suspended solids (MLVSS), biological oxygen demand (BOD), and chemical oxygen demand (COD). It was found that the biological degradation efficiency of the plant was below the desired levels in terms of COD and BOD. Also the plant operators were not maintaining consistent sludge retention time (SRT). Abrupt discharge of MLSS through the Surplus Activated sludge (SAS) pump was the main reason for the low MLSS in the aeration tank and consequently low treatment performance. In this study the SRT was optimized based on desired MLSS concentration between 3,000–3,500 mg/L and required performance in terms of BOD, COD and TSS. This study revealed that SRT is a very important operational parameter and its knowledge and correct implementation by the plant operators should be mandatory.

scholarly journals Niche Differentiation among Three Closely RelatedCompetibacteraceaeClades at a Full-Scale Activated Sludge Wastewater Treatment Plant and Putative Linkages to Process Performance

2018 ◽  
Vol 85 (5) ◽  
Author(s):  
Veronica R. Brand ◽  
Laurel D. Crosby ◽  
Craig S. Criddle
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Niche Differentiation ◽  
Full Scale ◽  
Pure Oxygen ◽  
Operational Parameters ◽  
Metabolic Potential ◽  
Operational Conditions

ABSTRACTMultiple clades within a microbial taxon often coexist within natural and engineered environments. Because closely related clades have similar metabolic potential, it is unclear how diversity is sustained and what factors drive niche differentiation. In this study, we retrieved three near-complete Competibacter lineage genomes from activated sludge metagenomes at a full-scale pure oxygen activated sludge wastewater treatment plant. The three genomes represent unique taxa within theCompetibacteraceae. A comparison of the genomes revealed differences in capacity for exopolysaccharide (EPS) biosynthesis, glucose fermentation to lactate, and motility. Using quantitative PCR (qPCR), we monitored these clades over a 2-year period. The clade possessing genes for motility and lacking genes for EPS biosynthesis (CPB_P15) was dominant during periods of suspended solids in the effluent. Further analysis of operational parameters indicate that the dominance of the CPB_P15 clade is associated with low-return activated sludge recycle rates and low wasting rates, conditions that maintain relatively high levels of biomass within the system.IMPORTANCEMembers of the Competibacter lineage are relevant in biotechnology as glycogen-accumulating organisms (GAOs). Here, we document the presence of threeCompetibacteraceaeclades in a full-scale activated sludge wastewater treatment plant and their linkage to specific operational conditions. We find evidence for niche differentiation among the three clades with temporal variability in clade dominance that correlates with operational changes at the treatment plant. Specifically, we observe episodic dominance of a likely motile clade during periods of elevated effluent turbidity, as well as episodic dominance of closely related nonmotile clades that likely enhance floc formation during periods of low effluent turbidity.

scholarly journals Time-series genome-centric analysis unveils bacterial response to operational disturbance in activated sludge

2019 ◽  
Author(s):  
María Victoria Pérez ◽  
Leandro D. Guerrero ◽  
Esteban Orellana ◽  
Eva L. Figuerola ◽  
Leonardo Erijman
Keyword(s):  
Growth Rate ◽  
Time Series ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Operating Conditions ◽  
The Face

ABSTRACTUnderstanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of three years, including a period of nine month of disturbance, characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons and thein situgrowth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single copy of the ribosomal RNA (rrn) operon. Despite only moderate differences in resource availability, replication rates were distributed uniformly throughout time, with no differences between disturbed and stable periods. We suggest that the length of the growth lag phase, rather than the growth rate, as the primary driver of selection under disturbed conditions. Thus, the system could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions.IMPORTANCEIn this work we investigated the response of microbial communities to disturbances in a full-scale activated sludge wastewater treatment plant over a time-scale that included periods of stability and disturbance. We performed a genome-wide analysis, which allowed us the direct estimation of specific cellular traits, including the rRNA operon copy number and the in situ growth rate of bacteria. This work builds upon recent efforts to incorporate growth efficiency for the understanding of the physiological and ecological processes shaping microbial communities in nature. We found evidence that would suggest that activated sludge could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions. This paper provides relevant insights into wastewater treatment process, and may also reveal a key role for growth traits in the adaptive response of bacteria to unsteady environmental conditions.

Simulation of a full-scale wastewater treatment plant performance at various temperatures using Extended Activated Sludge Model No.1

2021 ◽  
Vol 213 ◽  
pp. 190-201
Author(s):  
Noha A. El Hattab ◽  
Mostafa M. El-Seddik ◽  
Hisham S. Abdel-Halim ◽  
Minerva E. Matta
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Plant Performance ◽  
Activated Sludge Model

Full-scale control of Mycolata foam by FEX-120 addition

2010 ◽  
Vol 61 (10) ◽  
pp. 2443-2450 ◽  
Author(s):  
C. Kragelund ◽  
B. Nilsson ◽  
K. Eskilsson ◽  
A. M. Bøgh ◽  
P. H. Nielsen
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
No Reduction ◽  
Treatment Plant ◽  
Full Scale ◽  
Filamentous Bacteria ◽  
Gram Positive ◽  
Sludge Treatment ◽  
Scale Control

Foaming incidents in activated sludge treatment plants are a worldwide problem and occur on a regular basis in both municipal and industrial activated sludge treatment plants. Foaming is most often caused by excessive growth of filamentous bacteria, especially the gram-positive ones affiliated within the Actinobacteria, e.g. the branched Mycolata or CandidatusMicrothrix parvicella. Previous studies have shown that populations of Microthrix can be controlled by addition of certain polyaluminium compounds, but until now no effective chemicals have been identified to control other important foam formers such as the Mycolata. A new chemical (FilamentEx, FEX-120) was tested in full-scale in a Swedish wastewater treatment plant (WWTP) with immense foaming problems. In total, three different dosing events were carried out for more than 1 year. After only 8–17 weeks in each period, all foam had disappeared, and dosing of FEX-120 was stopped. Another 11 full-scale WWTPs in different countries were treated with FEX-120 because of severe Mycolata foaming on process tanks. In nine out of 11 plants, where the causative organisms were Gordonia or Skermania, a significant reduction of foam up to 100% was observed after treatment for approx. 10 weeks. In two WWTPs with unknown Mycolata organisms, no reduction was observed.

scholarly journals Time Series Genome-Centric Analysis Unveils Bacterial Response to Operational Disturbance in Activated Sludge

mSystems ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
María Victoria Pérez ◽  
Leandro D. Guerrero ◽  
Esteban Orellana ◽  
Eva L. Figuerola ◽  
Leonardo Erijman
Keyword(s):  
Growth Rate ◽  
Time Series ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Operating Conditions ◽  
Lag Phase

ABSTRACT Understanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of 3 years, including a 9-month period of disturbance characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons, and the in situ growth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single copy of the rRNA (rrn) operon. Despite moderate differences in resource availability, replication rates were distributed uniformly throughout time, with no differences between disturbed and stable periods. We suggest that the length of the growth lag phase, rather than the growth rate, is the primary driver of selection under disturbed conditions. Thus, the system could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions. IMPORTANCE Disturbance is a key determinant of community assembly and dynamics in natural and engineered ecosystems. Microbiome response to disturbance is thought to be influenced by bacterial growth traits and life history strategies. In this time series observational study, the response to disturbance of microbial communities in a full-scale activated sludge wastewater treatment plant was assessed by computing specific cellular traits of genomes retrieved from metagenomes. It was found that the genomes observed in disturbed periods have more copies of the rRNA operon than genomes observed in stable periods, whereas the in situ mean relative growth rates of bacteria present during stable and disturbed periods were indistinguishable. From these intriguing observations, we infer that the length of the lag phase might be a growth trait that affects the microbial response to disturbance. Further exploration of this hypothesis could contribute to better understanding of the adaptive response of microbiomes to unsteady environmental conditions.

Metagenomic analysis on seasonal microbial variations of activated sludge from a full-scale wastewater treatment plant over 4 years

2013 ◽  
Vol 6 (1) ◽  
pp. 80-89 ◽  
Author(s):  
Feng Ju ◽  
Feng Guo ◽  
Lin Ye ◽  
Yu Xia ◽  
Tong Zhang
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Metagenomic Analysis

Influence of aerators installation angles on process performance of an activated sludge in a full scale wastewater treatment plant, Kermanshah, Iran

2020 ◽  
Vol 207 ◽  
pp. 137-150
Author(s):  
Soheila Saki ◽  
Parviz Mohammadi ◽  
Ali Akbar Zinatizadeh ◽  
Seyyed Alireza Mousavi ◽  
Zahra Rahimi
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Process Performance

An activated sludge model based on activated sludge model number 3 for full‐scale wastewater treatment plant simulation

2009 ◽  
Vol 30 (7) ◽  
pp. 641-649 ◽  
Author(s):  
Ji Fan ◽  
Shu‐Guang Lu ◽  
Zhao‐fu Qiu ◽  
Xiao‐Xia Wang ◽  
Wen‐Zhen Li
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Activated Sludge Model ◽  
Model Based ◽  
Plant Simulation
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