scholarly journals Time-series genome-centric analysis unveils bacterial response to operational disturbance in activated sludge

2019 ◽  
Author(s):  
María Victoria Pérez ◽  
Leandro D. Guerrero ◽  
Esteban Orellana ◽  
Eva L. Figuerola ◽  
Leonardo Erijman
Keyword(s):  
Growth Rate ◽  
Time Series ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Operating Conditions ◽  
The Face

ABSTRACTUnderstanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of three years, including a period of nine month of disturbance, characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons and thein situgrowth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single copy of the ribosomal RNA (rrn) operon. Despite only moderate differences in resource availability, replication rates were distributed uniformly throughout time, with no differences between disturbed and stable periods. We suggest that the length of the growth lag phase, rather than the growth rate, as the primary driver of selection under disturbed conditions. Thus, the system could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions.IMPORTANCEIn this work we investigated the response of microbial communities to disturbances in a full-scale activated sludge wastewater treatment plant over a time-scale that included periods of stability and disturbance. We performed a genome-wide analysis, which allowed us the direct estimation of specific cellular traits, including the rRNA operon copy number and the in situ growth rate of bacteria. This work builds upon recent efforts to incorporate growth efficiency for the understanding of the physiological and ecological processes shaping microbial communities in nature. We found evidence that would suggest that activated sludge could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions. This paper provides relevant insights into wastewater treatment process, and may also reveal a key role for growth traits in the adaptive response of bacteria to unsteady environmental conditions.

scholarly journals Time Series Genome-Centric Analysis Unveils Bacterial Response to Operational Disturbance in Activated Sludge

mSystems ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
María Victoria Pérez ◽  
Leandro D. Guerrero ◽  
Esteban Orellana ◽  
Eva L. Figuerola ◽  
Leonardo Erijman
Keyword(s):  
Growth Rate ◽  
Time Series ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Operating Conditions ◽  
Lag Phase

ABSTRACT Understanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of 3 years, including a 9-month period of disturbance characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons, and the in situ growth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single copy of the rRNA (rrn) operon. Despite moderate differences in resource availability, replication rates were distributed uniformly throughout time, with no differences between disturbed and stable periods. We suggest that the length of the growth lag phase, rather than the growth rate, is the primary driver of selection under disturbed conditions. Thus, the system could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions. IMPORTANCE Disturbance is a key determinant of community assembly and dynamics in natural and engineered ecosystems. Microbiome response to disturbance is thought to be influenced by bacterial growth traits and life history strategies. In this time series observational study, the response to disturbance of microbial communities in a full-scale activated sludge wastewater treatment plant was assessed by computing specific cellular traits of genomes retrieved from metagenomes. It was found that the genomes observed in disturbed periods have more copies of the rRNA operon than genomes observed in stable periods, whereas the in situ mean relative growth rates of bacteria present during stable and disturbed periods were indistinguishable. From these intriguing observations, we infer that the length of the lag phase might be a growth trait that affects the microbial response to disturbance. Further exploration of this hypothesis could contribute to better understanding of the adaptive response of microbiomes to unsteady environmental conditions.

Nitrogen removal from wastewater in a pilot plant operated in the recirculation anoxic-oxic activated sludge mode

1996 ◽  
Vol 33 (12) ◽  
pp. 255-258 ◽  
Author(s):  
Olga Burica ◽  
Marjeta Strazar ◽  
Ivan Mahne
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Pilot Plant ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Volume Ratio ◽  
Mineral Nitrogen ◽  
Operating Conditions ◽  
Effluent Quality

The recirculation activated sludge process with preanoxic treatment was applied for biological reduction of the nitrogen content in municipal wastewater at pilot plant level. The pilot plant of total volume 3 300 1 with an initial anoxic to aerobic volume ratio of 40 : 60 was fed with wastewater from the first heavily loaded aerobic stage of a local wastewater treatment plant. Experiments were run over the summer and winter periods, the influent wastewater temperature being approx 24°C and approx 10°C, respectively. Special attention was paid to the hydraulic retention time, the total as well as mineral nitrogen loading, the aerobic to anaerobic volume ratio, and to the energy demand for denitrification of oxidised mineral nitrogen forms. Under optimal operating conditions the effluent quality that could be achieved was about 10 mg/l of total nitrogen (74% removal) and less than 2 mg N/l mineral nitrogen (87% removal), while simultaneously 205 mg BOD5/l in the influent was reduced to less than 7mg O2/l in the effluent. It was found feasible from the pilot plant experiments to upgrade an existing two stage aerobic-anaerobic wastewater treatment plant to reduce nitrogen from the liquid fraction of municipal wastewater so as to meet effluent quality standards without much additional volume and without amending the energy source for bioconversion of oxidised mineral nitrogen to gaseous forms.

scholarly journals Wastewater Treatment Plant Operation: Simple Control Schemes with a Holistic Perspective

2020 ◽  
Vol 12 (3) ◽  
pp. 768 ◽  
Author(s):  
S. Revollar ◽  
R. Vilanova ◽  
P. Vega ◽  
M. Francisco ◽  
M. Meneses
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Performance Indicator ◽  
Treatment Plant ◽  
Operating Conditions ◽  
Activated Sludge Process ◽  
Proportional Integral ◽  
Whole Plant ◽  
Global Performance

In this paper, a control approach for improving the overall efficiency of a wastewater treatment plant (WWTP) is presented. It consists of a cascaded control system that uses a global performance indicator as the controlled variable to drive the plant to operating conditions that satisfies trade-offs involved in the WWTP operation, improving the global performance of the plant. The selected global performance indicator is the N/E index that measures the ratio between the amount of nitrogenated compounds eliminated (kgN) and the energy (kWh) required to achieve that goal. This index links the variables of the activated sludge process with the energy consumed in the whole plant, thus the control strategy takes actions based on plantwide considerations. An external Proportional Integral (PI) controller changes the DO set point according to the N/E index and the basic dissolved oxygen (DO) control scheme in the activated sludge process follows this reference changes varying the aeration intensity. An outer loop with an event-based controller is used to compute the index values when the DO concentration is driven to excessively low limits, preventing long operation periods in this undesirable condition. Simple proportional integral controllers (PI) are used to adapt the strategy to the automation systems available in WWTPs. The implementation in the Benchmark Simulation Model 2 (BSM2) demonstrates the potential of the proposed approach. The results show the possibilities of the N/E index to be used as an indicator of global performance of WWTPs. It provides a link between water line objectives and energy consumption in the whole plant that can be exploited to introduce plantwide considerations in alternative control strategies formulated to drive the plant to operating conditions that optimize the overall process efficiency.

Rehabilitation and Upgrading of the Beni Suef City Wastewater Treatment Plant

1990 ◽  
Vol 22 (7-8) ◽  
pp. 131-138
Author(s):  
Ahmed Fadel
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Primary Treatment ◽  
High Rate ◽  
Shock Load ◽  
Economic Evaluations ◽  
Activated Sludge Process ◽  
Trickling Filter

Many of Egypt's cities have existing treatment plants under operation that have been constructed before 1970. Almost all of these treatment plants now need rehabilitation and upgrading to extend their services for a longer period. One of these plants is the Beni Suef City Wastewater Treatment Plant. The Beni Suef WWTP was constructed in 1956. It has primary treatment followed by secondary treatment employing intermediate rate trickling filters. The BOD, COD, and SS concentration levels are relatively high. They are approximately 800, 1100, and 600 mg/litre, respectively. The Beni Suef city required the determination of the level of work needed for the rehabilitation and upgrading of the existing 200 l/s plant and to extend its capacity to 440 l/s at year 2000 A description of the existing units, their deficiencies and operation problems, and the required rehabilitation are presented and discussed in this paper. Major problems facing the upgrading were the lack of space for expansion and the shortage of funds. It was, therefore, necessary to study several alternative solutions and methods of treatment. The choice of alternatives was from one of the following schemes: a) changing the filter medium, its mode of operation and increasing the number of units, b) changing the trickling filter to high rate and combining it with the activated sludge process, for operation by one of several possible combinations such as: trickling filter-solids contact, roughing filter-activated sludge, and trickling filter-activated sludge process, c) dividing the flow into two parts, the first part to be treated using the existing system and the second part to be treated by activated sludge process, and d) expanding the existing system by increasing the numbers of the different process units. The selection of the alternative was based on technical, operational and economic evaluations. The different alternatives were compared on the basis of system costs, shock load handling, treatment plant operation and predicted effluent quality. The flow schemes for the alternatives are presented. The methodology of selecting the best alternative is discussed. From the study it was concluded that the first alternative is the most reliable from the point of view of costs, handling shock load, and operation.

Operational Results of the Wolfsburg Wastewater Treatment Plant

1992 ◽  
Vol 25 (4-5) ◽  
pp. 203-209 ◽  
Author(s):  
R. Kayser ◽  
G. Stobbe ◽  
M. Werner
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Content ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Phosphate Removal ◽  
Total Nitrogen Content ◽  
Activated Sludge Process

At Wolfsburg for a load of 100,000 p.e., the step-feed activated sludge process for nitrogen removal is successfully in operation. Due to the high denitrification potential (BOD:TKN = 5:1) the effluent total nitrogen content can be kept below 10 mg l−1 N; furthermore by some enhanced biological phosphate removal about 80% phosphorus may be removed without any chemicals.

Management of toxicity effects in a large wastewater treatment plant

1997 ◽  
Vol 36 (2-3) ◽  
pp. 1-8 ◽  
Author(s):  
P. Grau ◽  
B. P. Da-Rin
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Severe Case ◽  
Treatment Efficiency ◽  
Filamentous Bulking ◽  
Biological Phenomena ◽  
Inhibition Of Nitrification ◽  
Toxicity Effects

An unusually severe case of toxicity accompanied by activated sludge filamentous bulking was observed at the wastewater treatment plant Sao Paulo-Barueri. Treatment efficiency of the plant, operated without major problems for more than five years before, was significantly hindered for almost six months. Occurrence of toxic shocks was confirmed partly directly but mostly indirectly by inhibition of nitrification and biological phenomena related to toxicity. Several measures adopted, including the recycled activated sludge chlorination, are described in the paper.

Automatic control for DO and pH in the activated sludge process in a coke wastewater treatment plant

1998 ◽  
Vol 37 (12) ◽  
pp. 141-148 ◽  
Author(s):  
B. K. Lee ◽  
S. W. Sung ◽  
H. D. Chun ◽  
J. K. Koo
Keyword(s):  
Wastewater Treatment ◽  
Control System ◽  
Activated Sludge ◽  
Automatic Control ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Pi Controller ◽  
Activated Sludge Process ◽  
Set Point ◽  
Coke Wastewater

The objective of this study is to develop an automatic control system for dissolved oxygen (DO) and pH of the activated sludge process in a coke wastewater treatment plant. A discrete type autotuned proportional-integral (PI) controller using an auto-regressive exogenous (ARX) model as a process model was developed to maintain the DO concentration in aerators by controlling the speed of surface aerators. Also a nonlinear pH controller using the titration curve was used to control the pH of influent wastewater. This control system was tested in a pilot scale plant. During this pilot plant experiment, there was small deviation of pH and the electric power consumption of surface aerators was reduced up to 70% with respect to the full operation when the DO set point was 2 mg/l. For real plant operation with this system, the discrete PI controller showed good tracking for set point change. The electricity saving was more than 40% of the electricity consumption when considering surface aerators. As a result of maintaining the DO constantly at the set point by the automatic control system, the fluctuation of effluent quality was decreased and overall improvement of the effluent water quality was achieved.

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