Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

AbstractAptamers are a kind of special nucleic acids that have the ability to bind various targets (e.g. small molecules, macromolecules, and cells) with high affinity and specificity. As a type of efficient recognition component in analytical designs and applications, aptamers have gained intense achievements in the designs of biosensors. Especially, the past few years have witnessed the fast development of electrochemical aptasensors. In this mini-review, we summarize recent progresses in electrochemical biosensors based on aptamers. Different targets and the corresponding detection principles are introduced. The methods to enhance detectable signals are mentioned including the employment of nanomaterials. Also, some possible limitations and future perspectives are discussed.

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Electrochemical biosensors for pathogen detection

Biosensors and Bioelectronics ◽

10.1016/j.bios.2020.112214 ◽

2020 ◽

Vol 159 ◽

pp. 112214 ◽

Cited By ~ 49

Author(s):

Ellen Cesewski ◽

Blake N. Johnson

Keyword(s):

Pathogen Detection ◽

Electrochemical Biosensors

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A Critical Review of Carbon Quantum Dots: From Synthesis toward Applications in Electrochemical Biosensors for the Determination of a Depression-Related Neurotransmitter

Materials ◽

10.3390/ma14143987 ◽

2021 ◽

Vol 14 (14) ◽

pp. 3987

Author(s):

Jingying Xu ◽

Jiangang Tao ◽

Lili Su ◽

Jidong Wang ◽

Tifeng Jiao

Keyword(s):

Quantitative Assessment ◽

Recent Progress ◽

Electrochemical Sensors ◽

Carbon Quantum Dots ◽

Synthetic Methods ◽

Therapeutic Interventions ◽

Electrochemical Biosensors ◽

Future Perspectives ◽

Human Fluids

Depression has become the leading cause of disability worldwide and is a global health burden. Quantitative assessment of depression-related neurotransmitter concentrations in human fluids is highly desirable for diagnosis, monitoring disease, and therapeutic interventions of depression. In this review, we focused on the latest strategies of CD-based electrochemical biosensors for detecting a depression-related neurotransmitter. We began this review with an overview of the microstructure, optical properties and cytotoxicity of CDs. Next, we introduced the development of synthetic methods of CDs, including the “Top-down” route and “Bottom-up” route. Finally, we highlighted detecting an application of CD-based electrochemical sensors in a depression-related neurotransmitter. Moreover, challenges and future perspectives on the recent progress of CD-based electrochemical sensors in depression-related neurotransmitter detection were discussed.

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Future Perspectives on New Approaches in Pathogen Detection

Biomedical Science Letters ◽

10.15616/bsl.2015.21.4.165 ◽

2015 ◽

Vol 21 (4) ◽

pp. 165-171 ◽

Cited By ~ 1

Author(s):

Peng Li ◽

Bow Ho ◽

Jeak Ling Ding

Keyword(s):

Pathogen Detection ◽

Future Perspectives ◽

New Approaches

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Electrochemical biosensors for detection of avian influenza virus--current status and future trends.

Acta Biochimica Polonica ◽

10.18388/abp.2014_1866 ◽

2014 ◽

Vol 61 (3) ◽

Cited By ~ 18

Author(s):

Iwona Grabowska ◽

Kamila Malecka ◽

Urszula Jarocka ◽

Jerzy Radecki ◽

Hanna Radecka

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Avian Influenza Virus ◽

Pathogen Detection ◽

Low Cost ◽

Virus Detection ◽

Small Sample ◽

Current Status ◽

Electrochemical Biosensors ◽

Influenza Virus Detection

Electrochemical biosensors have emerged as reliable analytical devices suitable for pathogen detection. Low cost, small sample requirement and possibility of miniaturization justifies their increasing development. Thus, we report in this review on the state of the art of avian influenza virus detection with genosensors and immunosensors working by an electrochemical mode. Their working principles focusing on the physical properties of the transducer, the immobilization chemistry, as well as new trends including incorporation of nanoparticles will be presented. Then, we critically review the detection of avian influenza virus in the complex matrices that use electrochemical biosensors and compare them with traditionally applied methods such as ELISA or Western blot.

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Electrochemical biosensors: State of the art and future perspectives in food analysis

Advanced Food Analysis Tools ◽

10.1016/b978-0-12-820591-4.00009-8 ◽

2021 ◽

pp. 167-185

Keyword(s):

Food Analysis ◽

State Of The Art ◽

Electrochemical Biosensors ◽

Future Perspectives

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Advances in Nanomaterials-Based Electrochemical Biosensors for Foodborne Pathogen Detection

Nanomaterials ◽

10.3390/nano11102700 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2700

Author(s):

Ivan Bobrinetskiy ◽

Marko Radovic ◽

Francesco Rizzotto ◽

Priya Vizzini ◽

Stefan Jaric ◽

...

Keyword(s):

Quantum Dots ◽

Foodborne Pathogens ◽

Pathogen Detection ◽

Carbon Nanomaterials ◽

Graphene Quantum Dots ◽

Foodborne Pathogen ◽

Electrochemical Biosensors ◽

Reduced Graphene ◽

Gold Silver ◽

2D And 3D Nanostructures

Electrochemical biosensors utilizing nanomaterials have received widespread attention in pathogen detection and monitoring. Here, the potential of different nanomaterials and electrochemical technologies is reviewed for the development of novel diagnostic devices for the detection of foodborne pathogens and their biomarkers. The overview covers basic electrochemical methods and means for electrode functionalization, utilization of nanomaterials that include quantum dots, gold, silver and magnetic nanoparticles, carbon nanomaterials (carbon and graphene quantum dots, carbon nanotubes, graphene and reduced graphene oxide, graphene nanoplatelets, laser-induced graphene), metal oxides (nanoparticles, 2D and 3D nanostructures) and other 2D nanomaterials. Moreover, the current and future landscape of synergic effects of nanocomposites combining different nanomaterials is provided to illustrate how the limitations of traditional technologies can be overcome to design rapid, ultrasensitive, specific and affordable biosensors.

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