Cellulase production by Aspergillus niger using urban lignocellulosic waste as substrate: Evaluation of different cultivation strategies

2022 ◽  
Vol 305 ◽  
pp. 114431
Author(s):  
Gislaine Brito Santos ◽  
Álvaro de Sousa Francisco Filho ◽  
Jacqueline Rêgo da Silva Rodrigues ◽  
Roberto Rodrigues de Souza
Keyword(s):  
Aspergillus Niger ◽  
Cellulase Production ◽  
Lignocellulosic Waste

PRODUCTION OF LIGNOCELLULOSES FROM ASPERGILLUS NIGER USING ARECA NUT SPADE AS BIOMASS..

2019 ◽  
pp. 1-3
Author(s):  
Madhuri B ◽  
Narasimha G ◽  
Balaji M*
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Cellulase Production ◽  
Areca Nut ◽  
Physical Treatment ◽  
Cellulose Production ◽  
Lactobionic Acid ◽  
Cellulase Enzyme

Areca palm (ChrysalidoCarpus lutescenes) a widely used plant having feathery arching brands with 100 leaflets. All these plants produce much of waste in additions to greeny and nuts. This waste of spade is used for the production of various molecules that are used in industry and pharma sector. Fermentation techniques are used to generate economically important enzymes for industrial and pharmaceutical purposes. Cellulase enzyme degrades the cellulose in between β-1, 4 glucosidic link found in lignocellulosic complex which under physical treatment is slower to degrade. The present study of Aspergillus niger for cellulose production was carried in solid state (SS) and submerged (SM) fermentations for production of cellulase enzyme. Cellulase production in SSF after 72 h of fermentation was 8.02 and in SMF activity was 2.98 per ml of cultured broth at H 6 and temperature at 30°C. Both SMF and SSF were supplemented with lactose and lactobionic acid, which acted as cellulase P production inducers. The aim of the present work was to study the effect of Areca palm spade as substrate for Aspergillus niger and its cellulase production under SMF and SSF.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

scholarly journals Chemical pretreatment of lignocellulosic wastes for cellulase production by Aspergillus niger FNU 6018

2019 ◽  
Author(s):  
Ida Bagus Wayan Gunam ◽  
Nyoman Semadi Antara ◽  
Anak Agung Made Dewi Anggreni ◽  
Yohanes Setiyo ◽  
I. Putu Eka Wiguna ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Cellulase Production ◽  
Chemical Pretreatment ◽  
Lignocellulosic Wastes

scholarly journals Optimization of Cellulase Enzyme Production by Co-cultures of Fungi Isolated from Lignocellulosic Waste

2020 ◽  
Vol 6 (5) ◽  
pp. 19-26
Author(s):  
Francis John V ◽  
Dr. Soloman P A
Keyword(s):  
Moisture Content ◽  
Incubation Time ◽  
Substrate Concentration ◽  
Enzyme Production ◽  
Trichoderma Viride ◽  
Cellulase Production ◽  
Optimal Conditions ◽  
Lignocellulosic Waste ◽  
Cellulase Enzyme ◽  
Degree Of Degradation

Fruit wastes were incubated with the mixture of cellulolytic fungi Penicillium citrinum, Aspergillus oryzae, and Trichoderma viride to hydrolyze the cellulosic components and to increase the degree of degradation. . The batch experiments are statistically designed and performed using Box-Benhken method of Response Surface Methodology to investigate the influence of major parameters viz., incubation time, temperature, pH, moisture content and substrate concentration on cellulase enzyme production. Maximum cellulase production of 2.03 Units/ml (U/ml) was detected by the RSM method in a mixed culture containing fungi at a ratio of 1: 1: 1 under optimal conditions at an incubation time of 5.27 days, a temperature of 34.09 °C, pH 4.85, moisture content of 63.83% and a substrate concentration of 5.03%.

scholarly journals Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology

2018 ◽  
Vol 25 (3) ◽  
pp. 115
Author(s):  
Abdullah Abdullah ◽  
Hamid Hamid ◽  
Marcelinus Christwardana ◽  
H. Hadiyanto
Keyword(s):  
Response Surface Methodology ◽  
Aspergillus Niger ◽  
Response Surface ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Lignocellulosic Material ◽  
Huge Amount ◽  
Activity Effect ◽  
Mgcl2 Concentration ◽  
Important Enzyme

Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap and already available in a huge amount. The objective of this research was to produce cellulase enzyme and to optimize it by using response surface methodology. The bagasse with water content of 80% was incubated with 2 ml inoculum of Aspergillus niger ITBCC L74 in a 250 ml Erlenmeyer flask. After reaching the specified time the enzyme was extracted and then determined for its activity. Effect of process parameters such as pH, urea and MgCl2 addition were studied. The optimal cellulase activity was achieved at urea concentration of 4.5% (w/w), MgCl2 concentration of 1 mM and pH of 3.5, with maximum enzyme activity was 0.630 U/gr.

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