Utilization of agricultural wastes as a substrate for Carboxymethyl cellulase production from Aspergillus niger in submerged fermentation

2011 ◽  
Vol 5 (5) ◽  
pp. 464 ◽  
Author(s):  
Muhammad Irfan ◽  
Usman Irfan ◽  
Zunaira Razaq ◽  
Quratulain Syed ◽  
Muhammad Nadeem
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Cellulase Production ◽  
Agricultural Wastes ◽  
Carboxymethyl Cellulase

Cellulase Production from Filamentous Fungi for Its Application in the Hydrolysis of Wheat Straw

2015 ◽  
Vol 1763 ◽  
Author(s):  
L. Toscano-Palomar ◽  
G. Montero-Alpirez ◽  
M. Stilianova-Stoytcheva ◽  
E. Vertiz-Pelaez ◽  
y E. Romero Uscanga
Keyword(s):  
Aspergillus Niger ◽  
Wheat Straw ◽  
Filamentous Fungi ◽  
Submerged Fermentation ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Mineral Medium ◽  
Cellulolytic Enzymes

ABSTRACTExtended research has been developed in the use of wheat straw (WS) as biomass for the production of biofuels (bioethanol), including the processes of degradation of cellulose by enzymatic systems. For centuries, Cellulose has been used by man; however, its enormous potential as a renewable energy source was recognized only after the discovery of cellulose degrading enzymes (cellulases). A wide variety of microorganisms can produce cellulolytic enzymes under appropriate culture conditions and among these microorganisms are filamentous fungi of the genera Trichoderma, Aspergillus, Penicillium and Fusarium. The purpose of this study was to produce cellulase enzyme from previously isolated and characterized filamentous fungi. Cellulytic fungi belonged toAspergillus flavus, Aspergillus niger, Aspergillus oryzae, Penicillium chrysogenum, Penicillium sp.,andTrichoderma harzianum.All these strains were preserved by lyophilization and also kept in sterile media (sand and soil) at 4 °C. The production of cellulases by submerged fermentation was performed in a Mandels mineral medium. The nitrogen sources were urea and ammonium sulfate. Glucose alone was used in the pre-inoculum, and dried and ground wheat straw was used in the fermentation as carbon sources. Subcultures of spore suspensions were incubated with orbital stirring (120 rpm) at 30 °C for 48 hours and used as inoculum for submerged fermentation with wheat straw as substrate in mineral medium with an initial pH of 5. Activity cellulase was determined by the method of 3,5-dinitrosalicylic acid (DNS). The results showed that wheat straw have potential for use as a substrate in the production of cellulases.Aspergillus nigershowed the highest enzymatic activity from the cellulase produced 0.051 FPU (filter paper units) after 96 hours of fermentation.

PRODUCTION OF LIGNOCELLULOSES FROM ASPERGILLUS NIGER USING ARECA NUT SPADE AS BIOMASS..

2019 ◽  
pp. 1-3
Author(s):  
Madhuri B ◽  
Narasimha G ◽  
Balaji M*
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Cellulase Production ◽  
Areca Nut ◽  
Physical Treatment ◽  
Cellulose Production ◽  
Lactobionic Acid ◽  
Cellulase Enzyme

Areca palm (ChrysalidoCarpus lutescenes) a widely used plant having feathery arching brands with 100 leaflets. All these plants produce much of waste in additions to greeny and nuts. This waste of spade is used for the production of various molecules that are used in industry and pharma sector. Fermentation techniques are used to generate economically important enzymes for industrial and pharmaceutical purposes. Cellulase enzyme degrades the cellulose in between β-1, 4 glucosidic link found in lignocellulosic complex which under physical treatment is slower to degrade. The present study of Aspergillus niger for cellulose production was carried in solid state (SS) and submerged (SM) fermentations for production of cellulase enzyme. Cellulase production in SSF after 72 h of fermentation was 8.02 and in SMF activity was 2.98 per ml of cultured broth at H 6 and temperature at 30°C. Both SMF and SSF were supplemented with lactose and lactobionic acid, which acted as cellulase P production inducers. The aim of the present work was to study the effect of Areca palm spade as substrate for Aspergillus niger and its cellulase production under SMF and SSF.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

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