Analgesic and anti-inflammatory activity of a crude root extract of Pfaffia glomerata (Spreng) Pedersen

2005 ◽  
Vol 96 (1-2) ◽  
pp. 87-91 ◽  
Author(s):  
A.G. Neto ◽  
J.M.L.C. Costa ◽  
C.C. Belati ◽  
A.H.C. Vinhólis ◽  
L.S. Possebom ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Root Extract ◽  
Pfaffia Glomerata ◽  
Anti Inflammatory

scholarly journals Anti-inflammatory and Anti-arthritic Potentials of Maesa Montana Roots

2021 ◽  
Vol 18 (16) ◽  
Author(s):  
Sanjida SHARMIN ◽  
Rabeya Gazi JHUMA ◽  
Sanjida ISLAM ◽  
Riniara KHATUN
Keyword(s):  
Bovine Serum ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Flowering Plant ◽  
Activity Measurement ◽  
Root Extract ◽  
Standard Drug ◽  
Activity Test ◽  
Anti Inflammatory

Maesa Montana is a flowering plant of the Myrsinaceae family, which is locally known as ramjani. The current study aimed to evaluate the in-vitro anti-arthritic and anti-inflammatory activities of the root extract of this plant. The anti-inflammatory performances were measured by the hindrance of egg white denaturation, and anti-arthritic activity was investigated by Bovine serum protein. In the anti-inflammatory activity test, the methanolic root extract of this plant showed 69.29 ± 1.19 % of inhibition at a concentration of 1000 µg/mL and standard drug exhibited 90.11 ± 1.45 % of inhibition at the same concentration. Furthermore, in the anti-arthritic activity test, the extract demonstrated 68.18 ± 1.34 % of inhibition at a concentration of 1000 µg/mL whereas the standard diclofenac drug showed 90.65 ± 1.19 % of inhibition at the same concentration. These results revealed that the root extract of this plant possesses significant anti-arthritic and anti-inflammatory activities based on the inhibition of BSA and protein denaturation. HIGHLIGHTS Methanol soluble compounds extraction from the roots of Maesa Montana Anti-inflammatory activity determination using egg albumin Anti-arthritic activity measurement using bovine serum albumin GRAPHICAL ABSTRACT

scholarly journals In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)

2018 ◽  
Vol 5 (10) ◽  
pp. 417-426 ◽  
Author(s):  
Samanjit Kaur ◽  
M. Syed Ali ◽  
V. Anuradha ◽  
V. Suganya ◽  
A. Ashashalini ◽  
...  
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Rhizophora Mucronata ◽  
Membrane Stabilization ◽  
In Vivo Models ◽  
Maximum Inhibition ◽  
Anti Inflammatory

In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.

Anti-inflammatory activity of tea (Camellia sinensis) root extract

Life Sciences ◽  
2004 ◽  
Vol 74 (15) ◽  
pp. 1839-1849 ◽  
Author(s):  
P Chattopadhyay ◽  
S.E Besra ◽  
A Gomes ◽  
M Das ◽  
P Sur ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Inflammatory Activity ◽  
Root Extract ◽  
Anti Inflammatory

scholarly journals EVALUATION OF ETHANOLIC ROOT EXTRACT OF PARTHENIUM HYSTEROPHORUS LINN FOR ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY

2017 ◽  
pp. 194-197 ◽  
Author(s):  
Pankaj Lohumi ◽  
Tirath Kumar ◽  
Lipi Nogai
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Parthenium Hysterophorus ◽  
Inflammatory Activity ◽  
Root Extract ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory

Objective: The work is aimed to draw out the health beneficial properties of a weed (Parthenium hysterophorus Linn). The present work is organized to evaluate the antioxidant and anti-inflammatory activity of the ethanolic root extract of Parthenium hysterophorus Linn.Methods: In the present work the ethanolic extract was determined by using soxhlet apparatus. The antioxidant scavenging activity of this extract was determined by applying three different assay methods: (1) DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical method. (2) Nitric oxide scavenging assay and (3) Reducing power method. The anti-inflammatory activity was determined by in vivo method i.e. Carrageenan induced rat paw edema.Results: DPPH radical scavenging activities of the standard antioxidant and extracts were found to be increased in dose dependent manner. The percentage inhibition increases from 4.19% to 97.09% within the concentration range of 10µg/ml to 160µg/ml. Parthenium hysterophorus Linn effectively reduced the generation of nitric oxide radicals from sodium nitroprusside solution in a concentration dependent manner and percentage inhibition increases from 3.53% to 55.21% within the concentration range 10µg/ml to 160µg/ml. All the concentrations of extract significantly showed higher absorbance than the absorbance of control reaction (0.9705) in reducing power assay. A Higher absorbance indicates high reducing power due to the formation of reduced intermediates. Parthenium hysterophorus Linn showed highly significant anti-inflammatory activity at a dose of 200 mg/kg and the lesser effect was observed at 100 mg/kg with the percentage change in paw volume at 0 min, 30 min, 60 min, 90 min and 120 min.Conclusion: Thus, from above experimental observations, it can be stated that Parthenium is a natural antioxidant and bearing anti-inflammatory activity. 

scholarly journals QUALITY CONTROL STANDARDIZATION AND ANTI-INFLAMMATORY ACTIVITY OF AYURVEDA FORMULATION YASHTIMADHU CHURNA

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Prince Kumar Pal ◽  
Biresh Kumar Sarkar ◽  
Harjeet Singh ◽  
Shashi Pal ◽  
Vijay Kumar ◽  
...  
Keyword(s):  
Quality Control ◽  
Protein Denaturation ◽  
Glycyrrhiza Glabra ◽  
Inflammatory Activity ◽  
Root Extract ◽  
Analysis Study ◽  
Anti Inflammatory ◽  
Chromatographic Fingerprinting

Yashtimadhu Churna is one of the traditional formulations used as antacid, anti-inflammatory, anticancer, antioxidant, analgesic, anti-stress, demulcent, antibacterial and anti-asthmatic agents. The formulation mainly contains Yastimadhu (Glycyrrhiza glabra) root extract. Present study described preliminary qualitative phytochemicals analysis and chromatographic fingerprinting of Yashtimadhu Churna using HPLC and HPTLC analysis. Study also presented anti-inflammatory potential of formulation using protein denaturation method. This study helps to establish standardization parameters of Yashtimadhu Churna and also confirmed anti-inflammatory potential of formulation.

scholarly journals Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract

2019 ◽  
pp. 62-68
Author(s):  
Ahmad Razi Othman ◽  
Intan Safinar Ismail ◽  
Norhani Abdullah ◽  
Syahida Ahmad
Keyword(s):  
Fatty Acid ◽  
Jatropha Curcas ◽  
Octadecadienoic Acid ◽  
Inflammatory Activity ◽  
Cytotoxic Activities ◽  
Preparative Hplc ◽  
Root Extract ◽  
Raw 264.7 ◽  
Carbon Fatty Acid ◽  
Anti Inflammatory

Jatropha curcas is a medicinal plant with many therapeutic properties such as anti-inflammatory, anti-malaria, anti-cancer and antioxidant. The root extract has been shown to possess high anti-inflammatory activity. Previously, the compounds responsible for this activity have not been fully elucidated. Two fractions (Fraction 1 and Fraction 2) obtained from a preparative HPLC of the root extract showed significant anti-inflammatory and cytotoxic activities in RAW 264.7 murine macrophage cells with Fraction 1 giving higher nitric oxide (NO) inhibition compared to Fraction 2 and L-NAME. Further purification steps involving column chromatography, thin layer chromatography and analytical HPLC of Fraction 1 produced two fractions labeled as Fraction A and Fraction B. Both fractions showed anti-inflammatory activity without cytotoxic activity in RAW 264.7 cells. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis showed that Fraction A contained a group of 18 carbon fatty acid compounds consisting of 2 oxooctadecanoic acids; 15, 16 dihydroxy 9Z, 12Z octadecadienoic acid; octadecadienoic isomer and 15,16 dihydroxy 9Z, 12Z octadecadienoic acid, 15S, 16S. The 18-carbon fatty acid structure was confirmed by nuclear magnetic resonance (NMR) spectral data. The IC50 value of compounds in Fraction A for anti-inflammatory activity in RAW 264.7 cell line was 434.8±0.75 µg/mL. From the analysis, it can be concluded that Fraction A can be classified under 18 carbon long chain fatty acid group based on LC MS/MS and NMR analysis. This active compound shows an inhibition towards NO activity.

Dandelion root extract affects ESCC progression via regulating multiple signal pathways

2021 ◽  
Author(s):  
Xiaofang Duan ◽  
Limin Pan ◽  
Yuying Deng ◽  
Ya Liu ◽  
Xue Han ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Signal Pathways ◽  
Root Extract ◽  
Edible Plant ◽  
Multiple Signal ◽  
Anti Inflammatory

Dandelion, a medicinal and edible plant, exhibits anti-inflammatory activity. The purpose of the present study was to investigate the inhibitory effectiveness of aqueous dandelion root extract (DRE) on esophageal squamous...

In vivo and in vitro anti-inflammatory activity of Harrisonia perforata root extract

2012 ◽  
Vol 4 (32) ◽  
pp. 38-44 ◽  
Author(s):  
Pattama Somsil ◽  
Nijsiri Ruangrungsi ◽  
Wacharee Limpanasitikul ◽  
Chandhanee Itthipanichpong
Keyword(s):  
Inflammatory Activity ◽  
Root Extract ◽  
Anti Inflammatory

scholarly journals In Vitro Anti-Inflammatory Activity in Arthritic Synoviocytes of A. brachypoda Root Extracts and Its Unusual Dimeric Flavonoids

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5219
Author(s):  
Carlota Salgado ◽  
Hugo Morin ◽  
Nayara Coriolano de Aquino ◽  
Laurence Neff ◽  
Cláudia Quintino da Rocha ◽  
...  
Keyword(s):  
High Speed ◽  
Inflammatory Activity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Root Extract ◽  
Traditional Use ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
Counter Current

Arrabidaea brachypoda is a plant commonly used for the treatment of kidney stones, arthritis and pain in traditional Brazilian medicine. Different in vitro and in vivo activities, ranging from antinociceptive to anti-Trypanosoma cruzi, have been reported for the dichloromethane root extract of Arrabidaea brachypoda (DCMAB) and isolated compounds. This work aimed to assess the in vitro anti-inflammatory activity in arthritic synoviocytes of the DCMAB, the hydroethanolic extract (HEAB) and three dimeric flavonoids isolated from the DCMAB. These compounds, brachydin A (1), B (2) and C (3), were isolated both by medium pressure liquid and high-speed counter current chromatography. Their quantification was performed by mass spectrometry on both DCMAB and HEAB. IL-1β activated human fibroblast-like synoviocytes were incubated with both extracts and isolated compounds to determine the levels of pro-inflammatory cytokine IL-6 by enzyme-linked immunosorbent assay (ELISA). DCMAB inhibited 30% of IL-6 release at 25 µg/mL, when compared with controls while HEAB was inactive. IC50 values determined for 2 and 3 were 3-fold higher than 1. The DCMAB activity seems to be linked to higher proportions of compounds 2 and 3 in this extract. These observations could thus explain the traditional use of A. brachypoda roots in the treatment of osteoarthritis.

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