QUALITY CONTROL STANDARDIZATION AND ANTI-INFLAMMATORY ACTIVITY OF AYURVEDA FORMULATION YASHTIMADHU CHURNA

Yashtimadhu Churna is one of the traditional formulations used as antacid, anti-inflammatory, anticancer, antioxidant, analgesic, anti-stress, demulcent, antibacterial and anti-asthmatic agents. The formulation mainly contains Yastimadhu (Glycyrrhiza glabra) root extract. Present study described preliminary qualitative phytochemicals analysis and chromatographic fingerprinting of Yashtimadhu Churna using HPLC and HPTLC analysis. Study also presented anti-inflammatory potential of formulation using protein denaturation method. This study helps to establish standardization parameters of Yashtimadhu Churna and also confirmed anti-inflammatory potential of formulation.

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Anti-inflammatory and Anti-arthritic Potentials of Maesa Montana Roots

Walailak Journal of Science and Technology (WJST) ◽

10.48048/wjst.2021.9564 ◽

2021 ◽

Vol 18 (16) ◽

Author(s):

Sanjida SHARMIN ◽

Rabeya Gazi JHUMA ◽

Sanjida ISLAM ◽

Riniara KHATUN

Keyword(s):

Bovine Serum ◽

Protein Denaturation ◽

Inflammatory Activity ◽

Flowering Plant ◽

Activity Measurement ◽

Root Extract ◽

Standard Drug ◽

Activity Test ◽

Anti Inflammatory

Maesa Montana is a flowering plant of the Myrsinaceae family, which is locally known as ramjani. The current study aimed to evaluate the in-vitro anti-arthritic and anti-inflammatory activities of the root extract of this plant. The anti-inflammatory performances were measured by the hindrance of egg white denaturation, and anti-arthritic activity was investigated by Bovine serum protein. In the anti-inflammatory activity test, the methanolic root extract of this plant showed 69.29 ± 1.19 % of inhibition at a concentration of 1000 µg/mL and standard drug exhibited 90.11 ± 1.45 % of inhibition at the same concentration. Furthermore, in the anti-arthritic activity test, the extract demonstrated 68.18 ± 1.34 % of inhibition at a concentration of 1000 µg/mL whereas the standard diclofenac drug showed 90.65 ± 1.19 % of inhibition at the same concentration. These results revealed that the root extract of this plant possesses significant anti-arthritic and anti-inflammatory activities based on the inhibition of BSA and protein denaturation. HIGHLIGHTS Methanol soluble compounds extraction from the roots of Maesa Montana Anti-inflammatory activity determination using egg albumin Anti-arthritic activity measurement using bovine serum albumin GRAPHICAL ABSTRACT

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In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs.051018 ◽

2018 ◽

Vol 5 (10) ◽

pp. 417-426 ◽

Cited By ~ 1

Author(s):

Samanjit Kaur ◽

M. Syed Ali ◽

V. Anuradha ◽

V. Suganya ◽

A. Ashashalini ◽

...

Keyword(s):

Protein Denaturation ◽

Inflammatory Activity ◽

Bark Extract ◽

Root Extract ◽

Rhizophora Mucronata ◽

Membrane Stabilization ◽

In Vivo Models ◽

Maximum Inhibition ◽

Anti Inflammatory

In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.

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Evaluation of Anti-Inflammatory Property of Maranta Arundinacea using Protein Denaturation Assay - An In vitro Study

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i47b33190 ◽

2021 ◽

pp. 834-840

Author(s):

Shilpa Merlyn Jose ◽

R. V. Geetha ◽

S. Rajeshkumar

Keyword(s):

Protein Denaturation ◽

Heart Diseases ◽

In Vitro Study ◽

Inflammatory Activity ◽

Root Extract ◽

Perennial Plant ◽

Arrow Root ◽

Anti Inflammatory ◽

Antibacterial And Antifungal

Introduction: Maranta arundinacea is a perennial plant which is seen in Mexico, central and South America. It was introduced and cultivated for its starch rich root which is known as arrowroot. It is gluten free and good for digestion. It also boasts metabolic rate and healthy sleep. Arrow root aid in lowering cholesterol which decreases the risk of heart diseases. It is a good substitute for breast milk because it’s loaded with magnesium, zinc and iron which promotes the growth and development of infants. Arrow root extract is previously known for its antibacterial and antifungal activity and also it gives relief from skin rashes, acne and skin sores. Aim: The aim of this study is to evaluate the anti-inflammatory property of Maranta arundinacea using protein denaturation assay. Materials and Methods: Maranta arundinacea (Arrowroot) was purchased commercially. Extract was prepared by adding 50 ml of distilled water and boiled at 50 degree Celsius and filtered. The extract was concentrated up to 10ml and the anti-inflammatory property was checked using protein denaturation assay and the readings were noted using a calorimeter. % of inhibition= ControlO.D-sampleO.D ∗100/ControlO.D. Results: It showed good anti inflammatory activity and thus it can be used as a potent alternative for anti inflammatory drugs. Conclusion: Maranta arundinacea extract showed good potential for anti-inflammatory activity. Thus in future more research can be done for formulations of eco-friendly treatment methods.

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Synthesis, Characterization and Evaluation of Anti-inflammatory Activity of Novel Indoline Derivatives

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v12i12.4826 ◽

2016 ◽

Vol 12 (12) ◽

pp. 4557-4563

Author(s):

Dhinesh kumar Manoharan

Keyword(s):

1H Nmr ◽

13C Nmr ◽

Protein Denaturation ◽

Inflammatory Activity ◽

Potent Inhibition ◽

Ft Ir ◽

Anti Inflammatory

Series of indoline derivatives were synthesized using N-(4-aminophenyl) indoline-1-carbothiamide as a precursor. The structures of synthesized compounds were confirmed by FT-IR, 1H-NMR, 13C-NMR and LC-MS. The in vitro anti-inflammatory activity of synthesized indoline derivatives were examined by standard anti-denaturation assay. The compounds 4a (IC50 = 62.2 µg/ml) and 4b (IC50 = 60.7 µg/ml) showed potent inhibition on protein denaturation. The compounds 5a (IC50 = 97.8 µg/ml) exhibits moderate inhibition on protein denaturation

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Anti-inflammatory activity of Punica granatum extract on oral microbes - In vitro

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i2.375 ◽

2019 ◽

Vol 10 (2) ◽

pp. 1019-1022

Author(s):

Westeros Dominic Pereira ◽

Geetha RV ◽

Lakshmi Thangavelu

Keyword(s):

Protein Denaturation ◽

Periodontal Diseases ◽

Punica Granatum ◽

Inflammatory Activity ◽

Oral Diseases ◽

Major Health Problem ◽

Maximum Inhibition ◽

Anti Inflammatory ◽

Oral Microbes

To study the anti-inflammatory effect of Punica granatum extract against the oral microbes. Oral diseases continue to be a major health problem worldwide. Dental caries and periodontal diseases are among the most important global oral health problems, although conditions such as oral and pharyngeal cancers and oral tissue lesions are also significant health concerns. Pomegranate extracts have been used for centuries in traditional medicine to confer health benefits in a number of inflammatory diseases, microbial infections and cancer. The anti-inflammatory activity of pomegranate extract was evaluated by protein denaturation assay, and the results were read spectrophotometrically. Denaturation of proteins is a great‐ documented cause of inflammation. As a part of the investigation on the mechanism of the anti-inflammatory activity, the ability to extract to inhibit protein denaturation was studied. It was effective in inhibiting heat induced albumin denaturation at different concentrations as shown in Table 1. Maximum inhibition, 70.12±1.12% was observed at500µg/ml. IC50 value was found to be 105.35±1.99µg/ml. Aspirin, a standard anti-inflammatory drug showed the maximum inhibition, 77.12±1.42% at the concentration of 200µg/ml. Hence it can be concluded that pomegranate extract has anti-inflammatory property and also can be used in products such as toothpaste and mouth wash etc.

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In vitro Evaluation of Anti-Inflammatory Activity of Symplocos racemosa Using Protein Denaturation Assay

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i47b33174 ◽

2021 ◽

pp. 715-720

Author(s):

K. Janani ◽

R. V. Geetha ◽

S. Rajeshkumar

Keyword(s):

Plant Extract ◽

Protein Denaturation ◽

Inflammatory Activity ◽

Whatman Filter Paper ◽

Traditional Drug ◽

Herbal Plants ◽

Anti Inflammatory ◽

Herbal Formulations ◽

Symplocos Racemosa

Introduction: Recently there is considerable awareness and interest in the field of herbal medicine due to its natural origin and lesser side effects compared to Allopathy. Selected herbal plants like Symplocos racemosa, commonly known as lodhra, are found mainly in plains and lower hills of Bengal. The word ‘Lodhra’ means ‘Propitious’. Symplocos racemosa is an important Indian traditional drug used in many Ayurvedic and herbal formulations for treatment of liver as well as uterine disorders and leucorrhea. Ethnobotanical Literature indicates use of Symplocos racemosa in treatment of eye disease, skin disease, ear disorders, liver and bowel complaints, tumours,uterine disorders, spongy and bleeding gums, asthma, fever, snakebite, gonorrhoea and arthritis. Aim: To analyse the anti-inflammatory activity of Symplocos racemosa using protein denaturation assay. Materials and Methods: 2 g of Lodhra bark powder is mixed with 100 ml distilled water & boiled for 20 min at 50°C. The extract is filtered using whatman filter paper & concentrated to 10 ml.1 ml each of Bovine serum albumin is added to various fixations of plant extract (10μL - 50 μL) and the anti - inflammatory activity was evaluated by analysing the percentage inhibition. Results: From this study, it is evident that Lodhra has significant anti-inflammatory activity. At 50μℓ concentration, the plant extract shower higher anti- inflammatory activity of 76%. Conclusion: Symplocos racemosa extract has proved to exhibit effective anti- inflammatory activity. Further studies have to be carried to analyse the other properties of this herb, which can be incorporated successfully in the pharmaceutical industry.

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ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA SILVER NANOPARTICLES: BIOCHEMICAL STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i2.29775 ◽

2019 ◽

pp. 346-349

Author(s):

GOMATHI KANNAYIRAM ◽

SANDHYA A ◽

SOWMIYA S ◽

VALARMATHI S ◽

DANIEL JOSEPH

Keyword(s):

Silver Nanoparticles ◽

Protein Denaturation ◽

Nigella Sativa ◽

Inflammatory Activity ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Infrared Analysis ◽

Standard Drug ◽

Rbc Membrane ◽

Anti Inflammatory

Objective: The aim of this study is to evaluate the anti-inflammatory activity of Nigella sativa silver nanoparticles (NS AgNPs). Methods: Fourier transform infrared analysis was used to characterize the NS AgNPs and the extract. 2,2-diphenylpicrylhydrazyl assay was done to test the antioxidant potency of NS AgNP. Furthermore, in vitro anti-inflammatory activity of the extract and the NS AgNP was determined by red blood cell (RBC) membrane stabilization assay, protein inhibition assay, and interleukin-1 (IL-1) beta assay. Results: The NS AgNP exhibited dose-dependent antioxidant property. At the concentration 0.01 mg/ml 80% of radical was scavenged by NS AgNP. Inhibition of protein denaturation assay also suggests that NS AgNP shows the highest activity (70%) when compared with the standard drug aspirin (65%). RBC assay suggests that NS AgNP stabilizes the RBC membrane and prevents leaking. In the enzyme-linked immunosorbent assay method the NS AgNP showed better IL-1 beta inhibition activity when compared to aqueous extract. Conclusion: From the study, it was inferred that NS AgNPs are more effective when compared to the extract. These results suggest that NS AgNP can be used to treat inflammatory disorders.

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ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i5.32512 ◽

2019 ◽

pp. 278-281

Author(s):

RAJESH A ◽

DOSS A ◽

TRESINA PS ◽

MOHAN VR

Keyword(s):

Methanol Extract ◽

Protein Denaturation ◽

In Vitro Models ◽

Inflammatory Activity ◽

Membrane Stabilization ◽

Standard Drug ◽

Inflammatory Agent ◽

Potential Source ◽

Anti Inflammatory

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action. Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin. Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml. Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

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FORMULATION AND EVALUATION OF TOPICAL HERBAL GEL CONTAINING INCLUSION COMPLEX OF CURCUMIN

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i9.34053 ◽

2019 ◽

pp. 196-201

Author(s):

VANDANA D ◽

SHWETA PAWAR

Keyword(s):

Inclusion Complex ◽

Protein Denaturation ◽

Release Kinetics ◽

Inflammatory Activity ◽

In Vitro Dissolution ◽

Diffusion Method ◽

Topical Gel ◽

In Vitro Diffusion ◽

Anti Inflammatory

Objective: The current work was aimed to prepare a topical gel containing curcumin (CUR) for the treatment of microbial infections on skin. Methods: CUR was complexed with the β-cyclodextrin (β-CD) using kneading method in 1:1, 1:2, and 1:3 molar ratios and characterized. The inclusion complex with high aqueous solubility was loaded in the topical gel containing (2% CUR) which was prepared using carbopol, sodium CMC, and guar gum and evaluated for viscosity, spreadability, extrudability, pH, drug content, and in vitro diffusion studies. The in vitro anti-inflammatory activity of the gel was performed by albumin protein denaturation technique, the statistical analysis was done using ANOVA followed Dunnett’s t-test. The antimicrobial activity of CUR was evaluated using standard strains of Candida albicans and Escherichia coli by agar well diffusion method. Results: The complexation of CUR had an increased solubility up to 103.09 times for 1:3 molar ratio with in vitro dissolution 90.64% for 60 min. The optimized formulation F9 had viscosity of 6500.3 cps and 97.5% in vitro drug diffusion for 8 h which follows zero-order release kinetics. In vitro anti-inflammatory activity studied showed that the CUR gel has a good potency for renaturation and was as effective as standard diclofenac with 76.9% inhibition (p=0.0507). CUR showed approx. 3 mm diameter of zone of inhibition against C. albicans and E. coli. Conclusion: A stable topical gel of CUR using β-CD and carbopol was successfully prepared which showed better in vitro diffusion with promising anti-inflammatory and antifungal action.

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