In vitro inhibitory effect of Streblus asper leaf-extract on adhesion of Candida albicans to human buccal epithelial cells

2005 ◽  
Vol 96 (1-2) ◽  
pp. 221-226 ◽  
Author(s):  
S. Taweechaisupapong ◽  
T. Choopan ◽  
S. Singhara ◽  
S. Chatrchaiwiwatana ◽  
S. Wongkham
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Buccal Epithelial Cells ◽  
Streblus Asper

scholarly journals Perbandingan Daya Hambat Larutan Antiseptik Povidone iodine dengan Ekstrak Daun Sirih terhadap Candida albicans secara In Vitro

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Septriana Putri ◽  
Aziz Djamal ◽  
Rahmatini Rahmatini ◽  
Cimi Ilmiawati
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Vaginal Discharge ◽  
Povidone Iodine ◽  
Inhibitory Effect ◽  
Iodine Solution ◽  
Betel Leaf ◽  
Post Hoc ◽  
Povidone Iodine Solution

Abstrak Candida albicansb (C. albicans) adalah salah satu mikroorganisme penyebab masalah kesehatan reproduksi wanita, yaitu keputihan (fluor albus). Penggunaan larutan povidone iodine dan bahan alam seperti ekstrak daun sirih menjadi pilihan masyarakat sebagai pembersih alat kewanitaan. Tujuan penelitian ini adalah untuk membandingkandaya hambat larutan antiseptik povidone iodine dan ekstrak daun sirih terhadap jamur C. albicans secara in vitro. Penelitian dilakukan terhadap lima isolat jamur C. albicans dengan larutan kontrol akuades.Perlakuan terdiri dari povidone iodine, ekstrak daun sirih dengan konsentrasi 5%, 10%, dan 20%.Hasil penelitian menunjukkan bahwa povidone iodine memiliki daya hambat terhadap C. albicans. Ekstrak daun sirih dengan konsentrasi 5% dan 10% tidak memiliki daya hambat terhadap C. albicans, namun ekstrak daun sirih konsentrasi 20% memiliki daya hambat terhadap C. albicans. Analisis statistik dengan uji ANOVA yang dilanjutkan dengan uji Post-hoc menunjukkan perbedaan bermakna antara daya hambat larutan povidone iodine dan ekstrak daun sirih 20% terhadap kontrol(p < 0.05).Larutan povidone iodine memiliki daya hambat dua kali lebih besar terhadap pertumbuhan C. albicans dibandingkan ekstrak daun sirih 20%. Dari penelitian ini dapat disimpulkan bahwa larutan povidone iodine dan ekstrak daun sirih 20% dapat menghambat pertumbuhan jamur C. albicans secara in vitro. Kata kunci: povidone iodine, ekstrak daun sirih, Candida albicansAbstract Candida albicans (C. albicans) is one of the frequent causes of  reproductive health problems in women, namely vaginal discharge (fluor albus). The antiseptic solution, povidone iodine, is still an option to overcome vaginal discharge. The use of natural materials such as betel (Piper betle L.) leaves extract also become a popular choice as adouche for women. The objective of this study was to compare the inhibitory activity of povidone iodine solution and betel leaf extract against the growth of C. albicans in vitro. We used five different isolates of C. albicans with distilled water as control. Each isolate was treated with povidone iodine solution, betel leaf extract at concentration of 5%, 10%,and 20%. The results showed that povidone iodine had inhibitory effect on C. albicans. Betel leaf extract at concentration of 5% and 10% did not have inhibitory effectwhile betel leaf extract at concentration of 20% hadinhibitory effect on C. albicans. Analysis by ANOVA followed by Post-hoc tests showed a significant difference in inhibitory activity of povidone iodine and betel leaf extract at 20% concentration compared to control (p < 0,05). Povidone iodine solution showed twice as much as inhibitory effect on C. albicans compared to betel leaf extract (20% concentration). It is concluded that povidone iodine solution and betel leaf extract at 20% concentration can inhibit the growth of C. albicans in vitro.Keywords: povidone iodine, betel leaf extract, Candida albicans

Inhibitory effect of Streblus asper leaf-extract on adhesion of Candida albicans to denture acrylic

2006 ◽  
Vol 106 (3) ◽  
pp. 414-417 ◽  
Author(s):  
S. Taweechaisupapong ◽  
P. Klanrit ◽  
S. Singhara ◽  
W. Pitiphat ◽  
S. Wongkham
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Streblus Asper

In vitro effect of Paullinia cupana (guaraná) on hydrophobicity, biofilm formation, and adhesion of Candida albicans’ to polystyrene, composites, and buccal epithelial cells

2015 ◽  
Vol 60 (3) ◽  
pp. 471-478 ◽  
Author(s):  
Ermelinda Matsuura ◽  
Janine Silva Ribeiro Godoy ◽  
Patrícia de Souza Bonfim-Mendonça ◽  
João Carlos Palazzo de Mello ◽  
Terezinha Inez Estivalet Svidzinski ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Biofilm Formation ◽  
Paullinia Cupana ◽  
Buccal Epithelial Cells ◽  
Vitro Effect

scholarly journals Uji daya hambat ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Candida albicans secara in vitro

e-GIGI ◽  
2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Olivia C. Simatupang ◽  
Jemmy Abidjulu ◽  
Krista V. Siagian
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Morinda Citrifolia ◽  
Predisposing Factor ◽  
Group Design ◽  
Post Test

Abstract: Candida albicans is a normal flora of the mouth, respiratory tract, gastrointestinal tract and the female genital but it becomes pathogen if there is a predisposing factor. Approximately 85-95% of oral candida infections are caused by C. albicans that is usually attached to the labial mucosa, buccal mucosa, the dorsal parts of the tongue and palate. Noni (Morinda citrifolia L.) is already known as a medical plant; one of it parts that has a medicinal effect is the leaf that contains anthraquinon as an antifungal. This study was aimed to determine the effect of noni leaf extract to the growth of C. albicans. This was a true experimental in vitro study with the post test only group design. The Kirby-Bauer method with filter paper was used to evaluate the sensitivity of C. albicans to mengkudu leaf extract. Leaves samples were extracted by using maceration process with ethanol 96%. C. albicans obtained from the pure stock of Laboratory of Microbiology Pharmacy Faculty of Math and Science, University of Sam Ratulangi. The results showed that the inhibition zone diameter of noni leaf extract on the growth of C. albicans was 16 mm (strong inhibition category). Conclusion: Noni leaf extract (Morinda citrifolia L.) had an inhibitory effect on Candida albicans.Keywords: Mengkudu leaf (Morinda citrifolia L.), Candida albicans, inhibition zone Abstrak: Candida albicans merupakan anggota flora normal rongga mulut, saluran pernafasan, saluran pencernaan, dan genital wanita namun dapat menjadi patogen jika terdapat faktor predisposisi. Sekitar 85-95% infeksi kandidiasis oral disebabkan oleh C albicans yang biasanya melekat pada mukosa labial, mukosa bukal, dorsum lidah dan palatum. Tanaman mengkudu (Morinda citrifolia L.) telah dikenal sebagai tumbuhan obat. Salah satu bagian tanaman mengkudu yang memiliki efek obat ialah daunnya karena adanya kandungan antrakuinon yang bersifat antijamur. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun mengkudu terhadap pertumbuhan C. albicans. Jenis penelitian ini ialah eksperimental laboratorik secara in vitro dengan post test only group design. Pengujian menggunakan metode Kirby-Bauer berbahan kertas saring. Sampel daun diekstraksi dengan proses maserasi menggunakan etanol 96%. Jamur C. albicans diambil dari stok biakan jamur murni Laboratorium Farmasi FMIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan diameter zona hambat ekstrak daun mengkudu terhadap pertumbuhan C. albicans 16 mm yang tergolong kriteria zona hambat kuat. Simpulan: Ekstrak daun mengkudu (Morinda citrifolia L.) mempunyai daya hambat terhadap jamur Candida albicans.Kata kunci: daun mengkudu, Candida albicans, zona hambat

scholarly journals Attenuation of Virulence and Changes in Morphology in Candida albicans by Disruption of theN-Acetylglucosamine Catabolic Pathway

2001 ◽  
Vol 69 (12) ◽  
pp. 7898-7903 ◽  
Author(s):  
Praveen Singh ◽  
Sharmistha Ghosh ◽  
Asis Datta
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Vital Role ◽  
Amino Sugars ◽  
Systemic Candidiasis ◽  
Catabolic Pathway ◽  
Pathway Gene ◽  
Buccal Epithelial Cells ◽  
Germ Tubes

ABSTRACT A Candida albicans mutant with mutations in theN-acetylglucosamine (GlcNAc) catabolic pathway gene cluster, including the GlcNAc-6-phosphate deacetylase (DAC1), glucosamine-6-phosphate deaminase (NAG1), and GlcNAc kinase (HXK1) genes, was not able to grow on amino sugars, exhibited highly attenuated virulence in a murine systemic candidiasis model, and was less adherent to human buccal epithelial cells in vitro. No germ tubes were formed by the mutant after induction with GlcNAc, but the mutant exhibited hyperfilamentation under stress-induced filamentation conditions. In addition, the GlcNAc catabolic pathway played a vital role in determining the colony phenotype. Our results imply that this pathway is very important because of its diverse links with pathways involved in virulence and morphogenesis of the organism.

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