Three Chinese herbal medicines promote neuroproliferation in vitro, and reverse the effects of chronic mild stress on behavior, the HPA axis, and proliferation of hippocampal precursor cell in vivo

The purpose of this study is to evaluate the effects of Chinese herbal medicines on the enzymatic activity of CYP3A4 and the possible metabolism-based herb-drug interactions in human liver microsomes and in rats. Fifty single-herbal preparations were screened for the activity of CYP3A4 using human liver microsomes for an in vitro probe reaction study. The enzymatic activity of CYP3A4 was estimated by determing the 6β-hydroxytestosterone metabolized from testosterone performed on a liquid chromatography-tandem mass spectrometry (LC-MS/MS). Huang Qin (Scutellaria baicalensis Geprgi), Mu Dan Pi (Paeonia suffruticosa Andr.), Ji Shiee Terng (Spatholobus suberectus Dunn.) and Huang Qi (Astragalus membranaceus [Fisch] Bge) have been demonstrated to have remarkable inhibiting effects on the metabolism of CYP3A4, whereas Xi Yi Hua (Magnolia biondii Pamp.) exhibited a moderate inhibition. These five single herbs were further investigated in an animal study using midazolam. Mu Dan Pi, Ji Shiee Terng and Huang Qi were observed to have greatly increased in the C max and AUC of midazolam. This study provides evidence of possible herb-drug interactions involved with certain single herbs.

Download Full-text

Effects of the Chinese Herbal Medicines Bupleuri radix, Ginseng radix, and Zingiberis rhizoma on Lymphatic Vessel Activity in Rats

The American Journal of Chinese Medicine ◽

10.1142/s0192415x06004521 ◽

2006 ◽

Vol 34 (06) ◽

pp. 1063-1068 ◽

Cited By ~ 5

Author(s):

Michiaki Yamakage ◽

Jun-Ichi Hattori ◽

Jun-Ichi Satoh ◽

Akiyoshi Namiki

Keyword(s):

Herbal Medicine ◽

Lymphatic Vessel ◽

Herbal Medicines ◽

In Vitro Study ◽

Chinese Herbal Medicines ◽

Chinese Herbal ◽

Radix Ginseng ◽

Bupleuri Radix

The aim of the present study was to determine the effects of the Chinese herbal medicines Bupleuri radix, Ginseng radix and Zingiberis rhizoma on spontaneous lymphatic vessel activity. The effect of each herbal medicine on in vivo lymphatic flow was examined by injection of dye into the femoral regions of rats after feeding with the herbal medicines. In an in vitro study, spontaneous changes in diameter of the rat thoracic duct were monitored, and each segment was exposed to each herbal medicine. In the in vivo study, 100% of the right iliac lymphatic node were positively stained in the herbal medicine group, whereas only 40% of the node were positively stained in the control group. In the in vitro study, Bupleuri radix and Ginseng radix increased the amplitude of spontaneous activity of lymphatic vessels in a concentration-dependent manner with or without L-NAME, an NO synthase inhibitor. The results indicated that the herbal medicines Bupleuri radix and Ginseng radix activated spontaneous lymphatic vasomotion and lymph flow, and the mechanisms of this effect seem to be independent of endothelial cells.

Download Full-text

In vitro anti-microbial and in vivo cytokine modulating effects of different prepared Chinese herbal medicines

Food and Chemical Toxicology ◽

10.1016/j.fct.2006.07.010 ◽

2006 ◽

Vol 44 (12) ◽

pp. 2078-2085 ◽

Cited By ~ 15

Author(s):

Shyh-Jye Lin ◽

Chung-Shih Chen ◽

Shih-Shen Lin ◽

Ming-Yung Chou ◽

Hung-Che Shih ◽

...

Keyword(s):

Herbal Medicines ◽

Chinese Herbal Medicines ◽

Chinese Herbal

Download Full-text

Emerging Role of Chinese Herbal Medicines in the Treatment of Pancreatic Fibrosis

The American Journal of Chinese Medicine ◽

10.1142/s0192415x1950037x ◽

2019 ◽

Vol 47 (04) ◽

pp. 709-726 ◽

Cited By ~ 2

Author(s):

Chang Liu ◽

Shuang Li ◽

Qingkai Zhang ◽

Fangyue Guo ◽

Mengying Tong ◽

...

Keyword(s):

Herbal Medicines ◽

Pancreatic Fibrosis ◽

Research Progress ◽

Common Disease ◽

Innovative Drug ◽

Chinese Herbal ◽

Pathologic Characteristic ◽

Prevention And Cure

Pancreatic fibrosis is the main pathologic characteristic in chronic pancreatitis (CP), a common disease that arises from surgery. Pancreatitis is caused by various etiologies, but the mechanism of fibrosis is not completely understood. Existing clinical approaches mainly focus on mitigating the symptoms and therefore do not cure the phenomena. In recent years, there has been a heightened interest in the use of Chinese herbal medicine (CHMs) in the prevention and cure of CP as expressed by increasing numbers of clinical and experimental research. Despite early cell culture and animal models, CHMs are able to interact with plenty of molecular targets involved in the pathogenesis of pancreatic fibrosis mostly via the TGF-[Formula: see text]/Smads pathway; however, integrated and up-to-date communication in this domain is unavailable. This review focuses on the research progress of CHMs against pancreatic fibrosis due to CP in vitro and in vivo and summarizes the potential mechanisms. We also outlined the toxicology of some CHMs for fibrosis treatment in order to provide a fuller understanding of drug safety. This review may provide reference for further innovative drug research and the future development of treatments for CP with pancreatic fibrosis.

Download Full-text

An in vitro study of neuroprotective properties of traditional Chinese herbal medicines thought to promote healthy ageing and longevity

BMC Complementary and Alternative Medicine ◽

10.1186/1472-6882-13-373 ◽

2013 ◽

Vol 13 (1) ◽

Cited By ~ 15

Author(s):

Bojiang Shen ◽

John Truong ◽

Ray Helliwell ◽

Suresh Govindaraghavan ◽

Nikolaus J Sucher

Keyword(s):

Herbal Medicines ◽

In Vitro Study ◽

Healthy Ageing ◽

Vitro Study ◽

Chinese Herbal Medicines ◽

Chinese Herbal ◽

Traditional Chinese Herbal Medicines

Download Full-text

Effect of Chinese herbal medicines on rumen fermentation, methanogenesis and microbial flora in vitro

South African Journal of Animal Science ◽

10.4314/sajas.v49i1.8 ◽

2019 ◽

Vol 49 (1) ◽

pp. 63 ◽

Cited By ~ 2

Author(s):

W.J. Wang ◽

S.P. Wang ◽

D.M. Luo ◽

X.L. Zhao ◽

M.J. Yin ◽

...

Keyword(s):

Herbal Medicines ◽

Rumen Fermentation ◽

Microbial Flora ◽

Chinese Herbal Medicines ◽

Chinese Herbal

Download Full-text

Ginsenoside Rb1 Induces a Pro-Neurogenic Microglial Phenotype via PPARγ Activation in Male Mice Exposed to Chronic Mild Stress

10.21203/rs.3.rs-269050/v1 ◽

2021 ◽

Author(s):

Zili You ◽

Lijuan Zhang ◽

Minmin Tang ◽

Xiaofang Xie ◽

Qiuying Zhao ◽

...

Keyword(s):

Chronic Mild Stress ◽

Hippocampal Neurogenesis ◽

Adult Hippocampal Neurogenesis ◽

Immunofluorescence Staining ◽

Mild Stress ◽

Ginsenoside Rb1 ◽

Male Mice ◽

Antidepressant Effects

Abstract BackgroundAnti-inflammatory approaches are emerging as a new strategy for treatment of depressive disorders. Ginsenoside Rb1 (GRb1), a major component of Panax ginseng, can inhibit inflammatory cascade and alleviate depressive behaviors. Microglia can promote or inhibit adult hippocampal neurogenesis according to their functional phenotypes. Here, we examined whether GRb1 may exert antidepressant effects by promoting a pro-neurogenic phenotype of microglia and thereby increasing neurogenesis. MethodsThe antidepressant effects of GRb1 or the licensed antidepressant imipramine (IMI) were assessed in chronic mild stress (CMS)-exposed male mice. The depressive-like behaviors of mice were evaluated by sucrose preference test, forced swimming test (FST), and tail suspension test (TST). The microglial phenotypes were identified by molecular markers and morphological properties, analyzed by RT-qPCR, western blotting and immunofluorescence staining. Effect of GRb1-treated microglia on adult hippocampal neurogenesis in vivo and in vitro were detected using immunofluorescence staining. ResultsBehavioral assessment indicated that GRb1 or IMI treatment alleviated depressive-like behaviors in CMS-exposed mice. Immunofluorescence examinationdemonstrated that GRb1 induced a pro-neurogenic phenotype of microglia via activating PPARγ in vivo and in vitro, which were reversed by PPARγ inhibitor GW9662. In addition, GRb1-treated microglia increased the proliferation and differentiation of neural precursor cells.ConclusionsThese findings demonstrated that GRb1 alleviated depressive-like behaviors of CMS-exposed male mice mainly through PPARγ-mediated microglial activation and improvement of adult hippocampus neurogenesis.

Download Full-text

Evaluation of in vitro embryotoxicity tests for Chinese herbal medicines

Reproductive Toxicology ◽

10.1016/j.reprotox.2019.06.001 ◽

2019 ◽

Vol 89 ◽

pp. 45-53 ◽

Cited By ~ 2

Author(s):

Lu Li ◽

Ling Yin Tang ◽

Bo Liang ◽

Rongyun Wang ◽

Qiuhua Sun ◽

...

Keyword(s):

Herbal Medicines ◽

Chinese Herbal Medicines ◽

Chinese Herbal

Download Full-text

The Potential of Chinese Herbal Medicines in the Treatment of Cervical Cancer

Integrative Cancer Therapies ◽

10.1177/1534735419861693 ◽

2019 ◽

Vol 18 ◽

pp. 153473541986169 ◽

Cited By ~ 5

Author(s):

Yi-Hsuan Hsiao ◽

Chiao-Wen Lin ◽

Po-Hui Wang ◽

Min-Chien Hsin ◽

Shun-Fa Yang

Keyword(s):

Cervical Cancer ◽

Treatment Options ◽

Herbal Medicines ◽

Antitumor Effects ◽

Clinical Safety ◽

Chinese Herbal Medicines ◽

Chinese Herbal ◽

Global Health Issue ◽

Underlying Mechanisms

Cervical cancer is a global health issue and places a considerable economic and medical burden on society. Thus, a concerted effort to improve the treatment of cervical cancer is warranted. Although several treatment options are currently available for treating patients with cervical cancer, such as chemoradiation and neoadjuvant or adjuvant chemotherapy, more aggressive systemic therapies and newer therapeutic agents are under investigation. Medicinal herbs have long been used to treat diseases. In this review, we summarize studies analyzing the antitumor effects and underlying mechanisms of Chinese herbal medicines, including the effects of crude extracts and compounds in vitro or in animal models for inducing apoptosis and inhibiting invasion or metastasis. Chinese herbal medicines with therapeutic targeting, such as those that interfere with tumor growth and progression in cervical cancer, have been widely investigated. To apply Chinese herbal medicine in the treatment of cervical cancer, adequate clinical studies are required to confirm its clinical safety and efficiency. Further investigations focused on the purification, pharmacokinetics, and identification of compounds from Chinese herbal medicines in cervical cancer treatment are necessary to achieve the aforementioned treatment goals.

Download Full-text