First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis

2017 ◽  
Vol 53 ◽  
pp. 30-37
Author(s):  
Carla Paola Bustos ◽  
Nora Guida ◽  
Almudena Casamayor ◽  
Alejandra Jimena Muñoz ◽  
José Francisco Fernández-Garayzábal ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
First Report ◽  
Streptococcus Equi

scholarly journals Molecular Characterization of Glycopeptide-Resistant Enterococcus faecium Isolates from Portuguese Hospitals

2005 ◽  
Vol 49 (7) ◽  
pp. 3073-3079 ◽  
Author(s):  
Carla Novais ◽  
João C. Sousa ◽  
Teresa M. Coque ◽  
Luísa V. Peixe
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Geographical Locations

ABSTRACT Fifty-one pulsed-field gel electrophoresis types and 17 Tn1546 variants were identified among 101 Enterococcus faecium isolates recovered in three distant Portuguese hospitals. Intra- and interhospital dissemination of specific strains and Tn1546 types was detected, which might largely contribute to the endemicity of vancomycin-resistant E. faecium in Portuguese hospitals, as happened previously in other geographical locations.

scholarly journals Molecular Characterization of Natural Erwinia pyrifoliae Strains Deficient in Hypersensitive Response

2003 ◽  
Vol 69 (1) ◽  
pp. 679-682 ◽  
Author(s):  
Susanne Jock ◽  
Won-Sik Kim ◽  
Marie-Anne Barny ◽  
Klaus Geider
Keyword(s):  
Molecular Characterization ◽  
Hypersensitive Response ◽  
Gel Electrophoresis ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Necrotic Tissue ◽  
Tobacco Leaves ◽  
Pulsed Field ◽  
Pear Tree

ABSTRACT From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with intact genes from E. pyrifoliae and Erwinia amylovora.

scholarly journals Erysipelothrix Rhusiopathiae Septicemia in a Laughing Kookaburra (Dacelo Novaeguineae)

2005 ◽  
Vol 17 (5) ◽  
pp. 497-499 ◽  
Author(s):  
T. Opriessnig ◽  
R. K. Vance ◽  
P. G. Halbur
Keyword(s):  
Weight Loss ◽  
Gel Electrophoresis ◽  
Bacterial Culture ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulmonary Congestion ◽  
Erysipelothrix Rhusiopathiae ◽  
Pulsed Field ◽  
First Report ◽  
New Genotype

Erysipelothrix rhusiopathiae (E. rhusiopathiae) septicemia was demonstrated in a captive Laughing kookaburra ( Dacelo novaeguineae). The bird died after a 2-week period of weakness and weight loss. At necropsy, the bird was emaciated and had reddened and wet lungs. Microscopic lesions were limited to hepatic and pulmonary congestion with focal thrombosis. Erysipelothrix rhusiopathiae was isolated by routine bacterial culture from several organs. Further characterization of the isolate by pulsed-field gel electrophoresis indicated that the isolate has a new genotype pattern 3A(III), which is 91.7% homologous to an E. rhusiopathiae that was isolated from a pig in 2001 and 88% homologous to an isolate recovered in 2000 from a turkey with septicemia. This is the first report of E. rhusiopathiae–induced septicemia in a kookaburra.

scholarly journals Molecular Characterization of Clostridium tetani Strains by Pulsed-Field Gel Electrophoresis and Colony PCR

2005 ◽  
Vol 71 (9) ◽  
pp. 5604-5606 ◽  
Author(s):  
Lucile Plourde-Owobi ◽  
Delphine Seguin ◽  
Marie-Anne Baudin ◽  
Catherine Moste ◽  
Bachra Rokbi
Keyword(s):  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Clostridium Tetani ◽  
Pulsed Field ◽  
Colony Pcr ◽  
First Time

ABSTRACT Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.

scholarly journals Molecular Characterization of Multidrug-ResistantStreptococcus pneumoniae Isolates in Korea

2000 ◽  
Vol 38 (4) ◽  
pp. 1641-1644 ◽  
Author(s):  
Jae-Hoon Song ◽  
Ji-won Yang ◽  
Joung Hwa Jin ◽  
Shin Woo Kim ◽  
Choon Kwan Kim ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Fingerprinting Analysis

Pulsed-field gel electrophoresis, ribotyping, and fingerprinting analysis of 22 invasive isolates of multidrug-resistant (MDR) pneumococci from Korea showed that 59 to 82% were genetically related. DNA sequencing of the PBP 2B gene showed relatively uniform alterations in nucleotides (5.4 to 7.8%) and amino acids (3.0 to 4.3%), while Asn-276→Lys, Arg-285→Cys and Ser-305→Phe substitutions were unique to Korean MDR strains, suggesting the spread of a few epidemic clones of resistant pneumococci within Korea.

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