scholarly journals Application of pulsed field gel electrophoresis for molecular characterization of vancomycin resistant Staphylococcus aureus isolates from hospitals

2020 ◽  
Vol 41 (2) ◽  
pp. 202-206
Author(s):  
A. Talwar ◽  
◽  
S. Kumar ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant

scholarly journals Molecular Characterization of Glycopeptide-Resistant Enterococcus faecium Isolates from Portuguese Hospitals

2005 ◽  
Vol 49 (7) ◽  
pp. 3073-3079 ◽  
Author(s):  
Carla Novais ◽  
João C. Sousa ◽  
Teresa M. Coque ◽  
Luísa V. Peixe
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Geographical Locations

ABSTRACT Fifty-one pulsed-field gel electrophoresis types and 17 Tn1546 variants were identified among 101 Enterococcus faecium isolates recovered in three distant Portuguese hospitals. Intra- and interhospital dissemination of specific strains and Tn1546 types was detected, which might largely contribute to the endemicity of vancomycin-resistant E. faecium in Portuguese hospitals, as happened previously in other geographical locations.

Detection of three distinct genetic lineages in methicillin-resistantStaphylococcus aureus(MRSA) isolates from animals and veterinary personnel

2010 ◽  
Vol 138 (5) ◽  
pp. 764-771 ◽  
Author(s):  
Y. ABBOTT ◽  
F. C. LEONARD ◽  
B. K. MARKEY
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Genetic Lineages ◽  
The Third

SUMMARYThis study involved the phenotypic and molecular characterization of a population of methicillin-resistantStaphylococcus aureusisolates from animals and from veterinary personnel in Ireland. Isolates from 77 animals (dogs,n=44; cats,n=4; horses,n=29) and from 28 veterinary personnel were characterized using their antimicrobial resistance profiles and pulsed-field gel electrophoresis patterns. In addition, a representative number of these isolates (n=52) were further analysed usingspa-typing techniques. The results obtained identified the presence of three distinct clonal complexes, CC5, CC8 and CC22, in both animal and human isolates. Two of these clonal complexes, CC8 and CC22, respectively, have been previously described in animals in Ireland but the presence of the third complex CC5 is a novel finding. The significance of this development, in relation to human and animal healthcare, is discussed.

scholarly journals Molecular Characterization of Natural Erwinia pyrifoliae Strains Deficient in Hypersensitive Response

2003 ◽  
Vol 69 (1) ◽  
pp. 679-682 ◽  
Author(s):  
Susanne Jock ◽  
Won-Sik Kim ◽  
Marie-Anne Barny ◽  
Klaus Geider
Keyword(s):  
Molecular Characterization ◽  
Hypersensitive Response ◽  
Gel Electrophoresis ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Necrotic Tissue ◽  
Tobacco Leaves ◽  
Pulsed Field ◽  
Pear Tree

ABSTRACT From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with intact genes from E. pyrifoliae and Erwinia amylovora.

First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis

2017 ◽  
Vol 53 ◽  
pp. 30-37
Author(s):  
Carla Paola Bustos ◽  
Nora Guida ◽  
Almudena Casamayor ◽  
Alejandra Jimena Muñoz ◽  
José Francisco Fernández-Garayzábal ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
First Report ◽  
Streptococcus Equi
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