scholarly journals Molecular Characterization of Natural Erwinia pyrifoliae Strains Deficient in Hypersensitive Response

2003 ◽  
Vol 69 (1) ◽  
pp. 679-682 ◽  
Author(s):  
Susanne Jock ◽  
Won-Sik Kim ◽  
Marie-Anne Barny ◽  
Klaus Geider
Keyword(s):  
Molecular Characterization ◽  
Hypersensitive Response ◽  
Gel Electrophoresis ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Necrotic Tissue ◽  
Tobacco Leaves ◽  
Pulsed Field ◽  
Pear Tree

ABSTRACT From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with intact genes from E. pyrifoliae and Erwinia amylovora.

Characterization of Erwinia amylovora Strains from Bulgaria by Pulsed-Field Gel Electrophoresis

2012 ◽  
Vol 67 ◽  
pp. 0187 ◽  
Author(s):  
Iliana Atanasova ◽  
Zoltan Urshev ◽  
Petya Hristova ◽  
Nevena Bogatzevska ◽  
Penka Moncheva
Keyword(s):  
Gel Electrophoresis ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Molecular Characterization of Glycopeptide-Resistant Enterococcus faecium Isolates from Portuguese Hospitals

2005 ◽  
Vol 49 (7) ◽  
pp. 3073-3079 ◽  
Author(s):  
Carla Novais ◽  
João C. Sousa ◽  
Teresa M. Coque ◽  
Luísa V. Peixe
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Geographical Locations

ABSTRACT Fifty-one pulsed-field gel electrophoresis types and 17 Tn1546 variants were identified among 101 Enterococcus faecium isolates recovered in three distant Portuguese hospitals. Intra- and interhospital dissemination of specific strains and Tn1546 types was detected, which might largely contribute to the endemicity of vancomycin-resistant E. faecium in Portuguese hospitals, as happened previously in other geographical locations.

First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis

2017 ◽  
Vol 53 ◽  
pp. 30-37
Author(s):  
Carla Paola Bustos ◽  
Nora Guida ◽  
Almudena Casamayor ◽  
Alejandra Jimena Muñoz ◽  
José Francisco Fernández-Garayzábal ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
First Report ◽  
Streptococcus Equi

scholarly journals Molecular Characterization of Clostridium tetani Strains by Pulsed-Field Gel Electrophoresis and Colony PCR

2005 ◽  
Vol 71 (9) ◽  
pp. 5604-5606 ◽  
Author(s):  
Lucile Plourde-Owobi ◽  
Delphine Seguin ◽  
Marie-Anne Baudin ◽  
Catherine Moste ◽  
Bachra Rokbi
Keyword(s):  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Clostridium Tetani ◽  
Pulsed Field ◽  
Colony Pcr ◽  
First Time

ABSTRACT Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.

scholarly journals Molecular Characterization of Multidrug-ResistantStreptococcus pneumoniae Isolates in Korea

2000 ◽  
Vol 38 (4) ◽  
pp. 1641-1644 ◽  
Author(s):  
Jae-Hoon Song ◽  
Ji-won Yang ◽  
Joung Hwa Jin ◽  
Shin Woo Kim ◽  
Choon Kwan Kim ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Fingerprinting Analysis

Pulsed-field gel electrophoresis, ribotyping, and fingerprinting analysis of 22 invasive isolates of multidrug-resistant (MDR) pneumococci from Korea showed that 59 to 82% were genetically related. DNA sequencing of the PBP 2B gene showed relatively uniform alterations in nucleotides (5.4 to 7.8%) and amino acids (3.0 to 4.3%), while Asn-276→Lys, Arg-285→Cys and Ser-305→Phe substitutions were unique to Korean MDR strains, suggesting the spread of a few epidemic clones of resistant pneumococci within Korea.

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