Ginsenosides repair UVB-induced skin barrier damage in BALB/c hairless mice and HaCaT keratinocytes

The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. The ability of repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, hyaluronic acid (HA), and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of matrix metalloproteinase-1 (MMP-1), since its induction by UVB treatment was diminished in treated CCD-986sk cells. Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption.

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Protective Effect of Indole-3-Pyruvate against Ultraviolet B-Induced Damage to Cultured HaCaT Keratinocytes and the Skin of Hairless Mice

PLoS ONE ◽

10.1371/journal.pone.0096804 ◽

2014 ◽

Vol 9 (5) ◽

pp. e96804 ◽

Cited By ~ 11

Author(s):

Reiji Aoki ◽

Ayako Aoki-Yoshida ◽

Chise Suzuki ◽

Yoshiharu Takayama

Keyword(s):

Protective Effect ◽

Ultraviolet B ◽

Hacat Keratinocytes ◽

Hairless Mice

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Recovery Effects of Oral Administration of Glucosylceramide and Beet Extract on Skin Barrier Destruction by UVB in Hairless Mice

Nutrients ◽

10.3390/nu9111178 ◽

2017 ◽

Vol 9 (11) ◽

pp. 1178 ◽

Cited By ~ 2

Author(s):

Yoshihiro Tokudome ◽

Noriomi Masutani ◽

Shohei Uchino ◽

Hisano Fukai

Keyword(s):

Oral Administration ◽

Skin Barrier ◽

Hairless Mice

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Intracellular Ca2+-Mediated AE2 Is Involved in the Vectorial Movement of HaCaT Keratinocyte

International Journal of Molecular Sciences ◽

10.3390/ijms21228429 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8429

Author(s):

Soyoung Hwang ◽

Dong Min Shin ◽

Jeong Hee Hong

Keyword(s):

Wound Healing ◽

Anion Exchanger ◽

Skin Barrier ◽

Chelating Agent ◽

Hacat Keratinocytes ◽

Keratinocyte Migration ◽

Enhanced Expression ◽

Hacat Keratinocyte ◽

Stimulation Of ◽

Exchange Activity

Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes.

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Involvement of skin barrier dysfunction in itch-related scratching in special diet-fed hairless mice

European Journal of Pharmacology ◽

10.1016/j.ejphar.2005.11.013 ◽

2006 ◽

Vol 530 (1-2) ◽

pp. 152-156 ◽

Cited By ~ 22

Author(s):

Masanori Fujii ◽

Takeshi Nabe ◽

Junko Tomozawa ◽

Shigekatsu Kohno

Keyword(s):

Skin Barrier ◽

Barrier Dysfunction ◽

Hairless Mice ◽

Special Diet

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Anti-Atopic Effect of Acorn Shell Extract on Atopic Dermatitis-Like Lesions in Mice and Its Active Phytochemicals

Biomolecules ◽

10.3390/biom10010057 ◽

2019 ◽

Vol 10 (1) ◽

pp. 57

Author(s):

Sullim Lee ◽

Hyun Jegal ◽

Sim-Kyu Bong ◽

Kyeong-No Yoon ◽

No-June Park ◽

...

Keyword(s):

Atopic Dermatitis ◽

Immunoglobulin E ◽

Inflammatory Diseases ◽

Skin Barrier ◽

Epidermal Thickness ◽

Barrier Dysfunction ◽

Hairless Mice ◽

Factor Α ◽

Ad Mouse Model ◽

Necrosis Factor

To investigate the potential effects of acorn shells on atopic dermatitis (AD), we utilized oxazolone (OX)- or 2,4-dinitrochlorobenzene (DNCB)-induced AD-like lesion mouse models. Our research demonstrates that Acorn shell extract (ASE) improved the progression of AD-like lesions, including swelling, which were induced by oxazolone on Balb/c mouse ears. Additionally, ASE significantly decreased the ear thickness (OX: 0.42 ± 0.01 mm, OX-ASE: 0.32 ± 0.02 mm) and epidermal thickness (OX: 75.3 ± 32.6 µm, OX-ASE: 46.1 ± 13.4 µm). The continuous DNCB-induced AD mouse model in SKH-1 hairless mice demonstrated that ASE improved AD-like symptoms, including the recovery of skin barrier dysfunction, Immunoglobulin E hyperproduction (DNCB: 340.1 ± 66.8 ng/mL, DNCB-ASE: 234.8 ± 32.9 ng/mL) and an increase in epidermal thickness (DNCB: 96.4 ± 21.9 µm, DNCB-ASE: 52.4 ± 16.3 µm). In addition, we found that ASE suppressed the levels of AD-involved cytokines, such as Tumor Necrosis Factor α, IL-1β, IL-25 and IL-33 in both animal models. Furthermore, gallic acid and ellagic acid isolated from ASE suppressed β-hexosaminidase release and IL-4 expression in RBL-2H3 cells. The acorn shell and its active phytochemicals have potential as a new remedy for the improvement of atopic dermatitis and other inflammatory diseases.

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