Clinical characteristics and molecular analysis of USA300 and ST 764 methicillin-resistant Staphylococcus aureus isolates from outpatients in Japan by PCR-Based open reading frame typing

2020 ◽  
Author(s):  
Shinji Ogihara ◽  
Osamu Inoue ◽  
Takaya Yamagami ◽  
Keita Yanagimoto ◽  
Kousei Uematsu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Analysis ◽  
Clinical Characteristics ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Open Reading Frame ◽  
Methicillin Resistant ◽  
Reading Frame

scholarly journals Molecular characterization of healthcare and community-associated methicillin-resistant Staphylococcus aureus using phage open-reading frame typing

2021 ◽  
Author(s):  
Takaaki Konuma ◽  
Shunsuke Takahashi ◽  
Masato Suzuki ◽  
Arinobu Tojo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Open Reading Frame ◽  
Methicillin Resistant ◽  
Reading Frame ◽  
Polymerase Chain ◽  
Healthcare Associated

The polymerase chain reaction-based open reading frame typing (POT) method is a simple and rapid method for the strain-level discrimination of methicillin-resistant Staphylococcus aureus (MRSA). We investigated the molecular charac- teristics of S. aureus strains by multilocus sequencing typing (MLST) and POT and the profiles of antibiotic resistance and virulence genes of MRSA isolates in a single center of Tokyo, Japan. Five types by MLST and 19 types by POT were detected in the 25 MRSA isolates. ST5 and a POT1 score of 93 were associated with healthcare-associated MRSA, whereas ST8 and a POT1 score of 106 were associated with community-associated MRSA. Each strain evaluated by POT score was completely associated with similar profiles of antibiotic resistance and virulence genes. These data showed that the POT system was a powerful molecular tool for the epidemiological characterization of MRSA isolates, which correlated with the profiles of antibiotic resistance and virulence genes.  

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