scholarly journals Rapid Identification of Methicillin-Resistant Staphylococcus aureus Transmission in Hospitals by Use of Phage-Derived Open Reading Frame Typing Enhanced by Multiplex PCR and Reverse Line Blot Assay

2010 ◽  
Vol 48 (8) ◽  
pp. 2741-2748 ◽  
Author(s):  
M. V. N. O'Sullivan ◽  
F. Kong ◽  
V. Sintchenko ◽  
G. L. Gilbert
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Rapid Identification ◽  
Reverse Line Blot ◽  
Open Reading Frame ◽  
Methicillin Resistant ◽  
Reading Frame ◽  
Reverse Line Blot Assay

scholarly journals Molecular characterization of healthcare and community-associated methicillin-resistant Staphylococcus aureus using phage open-reading frame typing

2021 ◽  
Author(s):  
Takaaki Konuma ◽  
Shunsuke Takahashi ◽  
Masato Suzuki ◽  
Arinobu Tojo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Open Reading Frame ◽  
Methicillin Resistant ◽  
Reading Frame ◽  
Polymerase Chain ◽  
Healthcare Associated

The polymerase chain reaction-based open reading frame typing (POT) method is a simple and rapid method for the strain-level discrimination of methicillin-resistant Staphylococcus aureus (MRSA). We investigated the molecular charac- teristics of S. aureus strains by multilocus sequencing typing (MLST) and POT and the profiles of antibiotic resistance and virulence genes of MRSA isolates in a single center of Tokyo, Japan. Five types by MLST and 19 types by POT were detected in the 25 MRSA isolates. ST5 and a POT1 score of 93 were associated with healthcare-associated MRSA, whereas ST8 and a POT1 score of 106 were associated with community-associated MRSA. Each strain evaluated by POT score was completely associated with similar profiles of antibiotic resistance and virulence genes. These data showed that the POT system was a powerful molecular tool for the epidemiological characterization of MRSA isolates, which correlated with the profiles of antibiotic resistance and virulence genes.  

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