A study on the mechanism of pore formation through VIPS-NIPS technique for membrane fabrication

2021 ◽  
Author(s):  
Amin Dehban ◽  
Fatemeh Hosseini Saeedavi ◽  
Ali Kargari
Keyword(s):  
Pore Formation ◽  
Membrane Fabrication

Optimization of PES Nanocomposite Membrane Preparation Using Statistical Analysis and Experimental Design

2019 ◽  
Author(s):  
Yasin Orooji ◽  
Fatemeh Noorisafa ◽  
Nahid Imami ◽  
Amir R. Chaharmahali
Keyword(s):  
Statistical Analysis ◽  
Experimental Design ◽  
Factorial Design ◽  
Fractional Factorial Design ◽  
Fractional Factorial ◽  
Nanocomposite Membrane ◽  
Membrane Preparation ◽  
Membrane Fabrication

<p>Using experimental design and statistical analysis (½ Fractional Factorial Design), this study investigates the effect of different parameters in the membrane fabrication on the performance of nanocomposite PES/TiO<sub>2</sub> membrane. </p>

Dimerization of the Antimicrobial Peptide Polyphemusin I into One Polypeptide Chain: Theoretical and Practical Consequences

2019 ◽  
Vol 35 (5) ◽  
pp. 36-41
Author(s):  
V.A. Zenin ◽  
E.G. Sadykhov ◽  
A.N. Fedorov
Keyword(s):  
Antimicrobial Peptides ◽  
Pore Formation ◽  
Lipid Membrane ◽  
Polypeptide Chain ◽  
Unique Identifier ◽  
Bacterial Cultures ◽  
In Series ◽  
The Russian Federation ◽  
Peptide Dimerization ◽  
Putative Mechanism

A strategy of sequential dimerization of monomers of antimicrobial peptides (AMPs) into one polypeptide chain has been implemented on the example of a beta-structural AMP polyphemusin I which is one of the most effective candidate for use as an antibiotic. The possible polyphemusin I monomer and dimer structures in lipid membrane were studied in this work via molecular modeling. To this end, these molecules were chemically synthesized so that the dimer represented two monomers connected in series into one polypeptide chain with a flexible linker. The antimicrobial effects of monomer and dimer were then tested on various bacterial cultures, and their similarity was shown. Therefore, we can conclude that the pore formation is not a putative mechanism of the polyphemusin I action. antimicrobial peptides, peptide dimerization, mechanism of antimicrobial action, polyphemusin The work was supported by the Ministry of Science and Higher Education of the Russian Federation (Project Unique Identifier RFMEFI57517X0151).

scholarly journals A Comprehensive Review of Polymeric Wastewater Purification Membranes

2021 ◽  
Vol 5 (6) ◽  
pp. 162
Author(s):  
Rasmeet Singh ◽  
Mandeep Singh ◽  
Nisha Kumari ◽  
Janak ◽  
Sthitapragyan Maharana ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Pore Formation ◽  
Inorganic Materials ◽  
Special Focus ◽  
Nanocomposite Membranes ◽  
Wastewater Purification ◽  
Comprehensive Review ◽  
Synthetic Membranes ◽  
Purification Membranes ◽  
Thermal And Chemical Stability

Synthetic membranes are currently employed for multiple separation applications in various industries. They may have been prepared from organic or inorganic materials. Present research majorly focuses on polymeric (i.e., organic) membranes because they show better flexibility, pore formation mechanism, and thermal and chemical stability, and demand less area for installation. Dendritic, carbon nanotube, graphene and graphene oxide, metal and metal oxide, zwitter-ionic, and zeolite-based membranes are among the most promised water treatment membranes. This paper critically reviews the ongoing developments to utilize nanocomposite membranes to purify water. Various membranes have been reported to study their resistance and fouling properties. A special focus is given towards multiple ways in which these nanocomposite membranes can be employed. Therefore, this review provides a platform to develop the awareness of current research and motivate its readers to make further progress for utilizing nanocomposite membranes in water purification.

Mechanistic Insights into Pore Formation by an α-Pore Forming Toxin: Protein and Lipid Bilayer Interactions of Cytolysin A

2020 ◽  
Vol 54 (1) ◽  
pp. 120-131
Author(s):  
Pradeep Sathyanarayana ◽  
Sandhya S. Visweswariah ◽  
K. Ganapathy Ayappa
Keyword(s):  
Lipid Bilayer ◽  
Pore Formation ◽  
Toxin Protein

scholarly journals Pore-forming Esx proteins mediate toxin secretion by Mycobacterium tuberculosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Uday Tak ◽  
Terje Dokland ◽  
Michael Niederweis
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pore Formation ◽  
Molecular Switches ◽  
Water Soluble ◽  
Host Cells ◽  
Solvent Exposure ◽  
Membrane Channel ◽  
Toxin Secretion ◽  
Outer Membrane Channel ◽  
Membrane Spanning

AbstractMycobacterium tuberculosis secretes the tuberculosis necrotizing toxin (TNT) to kill host cells. Here, we show that the WXG100 proteins EsxE and EsxF are essential for TNT secretion. EsxE and EsxF form a water-soluble heterodimer (EsxEF) that assembles into oligomers and long filaments, binds to membranes, and forms stable membrane-spanning channels. Electron microscopy of EsxEF reveals mainly pentameric structures with a central pore. Mutations of both WXG motifs and of a GXW motif do not affect dimerization, but abolish pore formation, membrane deformation and TNT secretion. The WXG/GXW mutants are locked in conformations with altered thermostability and solvent exposure, indicating that the WXG/GXW motifs are molecular switches controlling membrane interaction and pore formation. EsxF is accessible on the bacterial cell surface, suggesting that EsxEF form an outer membrane channel for toxin export. Thus, our study reveals a protein secretion mechanism in bacteria that relies on pore formation by small WXG proteins.

Single Vesicle Analysis Reveals Nanoscale Membrane Curvature Selective Pore Formation in Lipid Membranes by an Antiviral α-Helical Peptide

Nano Letters ◽  
2012 ◽  
Vol 12 (11) ◽  
pp. 5719-5725 ◽  
Author(s):  
Seyed R. Tabaei ◽  
Michael Rabe ◽  
Vladimir P. Zhdanov ◽  
Nam-Joon Cho ◽  
Fredrik Höök
Keyword(s):  
Lipid Membranes ◽  
Pore Formation ◽  
Membrane Curvature ◽  
Helical Peptide ◽  
Single Vesicle
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