Polymerase eta Is a Short-lived, Proteasomally Degraded Protein that Is Temporarily Stabilized Following UV Irradiation in Saccharomyces cerevisiae

2007 ◽  
Vol 366 (4) ◽  
pp. 1074-1086 ◽  
Author(s):  
Adrianna Skoneczna ◽  
Justyna McIntyre ◽  
Marek Skoneczny ◽  
Zofia Policinska ◽  
Ewa Sledziewska-Gojska
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Uv Irradiation ◽  
Polymerase Eta

scholarly journals A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Bernadette Connors ◽  
Lauren Rochelle ◽  
Asela Roberts ◽  
Graham Howard
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Excision Repair ◽  
Uv Irradiation ◽  
Double Mutant ◽  
Excision Repair ◽  
Strand Break ◽  
Anaphase Promoting Complex ◽  
End Joining ◽  
Ubiquitin Ligase Complex ◽  
Nucleotide Excision

Regulation of DNA repair can be achieved through ubiquitin-mediated degradation of transiently induced proteins. In Saccharomyces cerevisiae, Rad4 is involved in damage recognition during nucleotide excision repair (NER) and, in conjunction with Rad23, recruits other proteins to the site of damage. We identified a synthetic interaction upon UV exposure between Rad4 and Cdc20, a protein that modulates the activity of the anaphase promoting complex (APC/C), a multisubunit E3 ubiquitin ligase complex. The moderately UV sensitive Δrad4 strain became highly sensitive when cdc20-1 was present, and was rescued by overexpression of CDC20. The double mutant is also deficient in elicting RNR3-lacZ transcription upon exposure to UV irradiation or 4-NQO compared with the Δrad4 single mutant. We demonstrate that the Δrad4/cdc20-1 double mutant is defective in double strand break repair by way of a plasmid end-joining assay, indicating that Rad4 acts to ensure that damaged DNA is repaired via a Cdc20-mediated mechanism. This study is the first to present evidence that Cdc20 may play a role in the degradation of proteins involved in nucleotide excision repair.

scholarly journals Transcriptomic analysis and driver mutant prioritization for differentially expressed genes from a Saccharomyces cerevisiae strain with high glucose tolerance generated by UV irradiation

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 38784-38797 ◽  
Author(s):  
Ying Chen ◽  
Zhilong Lu ◽  
Dong Chen ◽  
Yutuo Wei ◽  
Xiaoling Chen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Tolerance ◽  
Differentially Expressed Genes ◽  
Uv Irradiation ◽  
High Glucose ◽  
Mutant Phenotype ◽  
Differentially Expressed ◽  
Driver Mutations ◽  
High Sugar ◽  
Sugar Tolerance

Driver mutations of a Saccharomyces cerevisiae mutant phenotype strain with high sugar tolerance were sought by the PheNetic network.

scholarly journals The essential DNA polymerases delta and epsilon are involved in repair of UV-damaged DNA in the yeast Saccharomyces cerevisiae.

1999 ◽  
Vol 46 (2) ◽  
pp. 289-298 ◽  
Author(s):  
A Hałas ◽  
Z Policińska ◽  
H Baranowska ◽  
W J Jachymczyk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Uv Irradiation ◽  
Dna Polymerases ◽  
Relative Molecular Mass ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Mutant Strains ◽  
Cellular Dna

We have studied the ability of yeast DNA polymerases to carry out repair of lesions caused by UV irradiation in Saccharomyces cerevisiae. By the analysis of postirradiation relative molecular mass changes in cellular DNA of different DNA polymerases mutant strains, it was established that mutations in DNA polymerases delta and epsilon showed accumulation of single-strand breaks indicating defective repair. Mutations in other DNA polymerase genes exhibited no defects in DNA repair. Thus, the data obtained suggest that DNA polymerases delta and epsilon are both necessary for DNA replication and for repair of lesions caused by UV irradiation. The results are discussed in the light of current concepts concerning the specificity of DNA polymerases in DNA repair.

Differential repair of UV damage in rad mutants of Saccharomyces cerevisiae: a possible function of G2 arrest upon UV irradiation

1990 ◽  
Vol 10 (9) ◽  
pp. 4678-4684
Author(s):  
C Terleth ◽  
P Schenk ◽  
R Poot ◽  
J Brouwer ◽  
P van de Putte
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Uv Irradiation ◽  
Uv Damage ◽  
G2 Arrest ◽  
Rad Mutants

After UV irradiation, the transcriptionally active MAT alpha locus in Saccharomyces cerevisiae is preferentially repaired compared with the inactive HML alpha locus. The effect of rad mutations from three different epistasis groups on differential repair was investigated. Three mutants, rad9, rad16, and rad24, were impaired in the removal of UV dimers from the inactive HML alpha locus, whereas they had generally normal repair of the active MAT alpha locus. Since RAD9 is necessary for G2 arrest after UV irradiation, we propose that the G2 stage plays a role in making the dimers accessible for repair, at least in the repressed HML alpha locus.

Ectopic recombination between Ty elements in Saccharomyces cerevisiae is not induced by DNA damage

1992 ◽  
Vol 12 (10) ◽  
pp. 4441-4448
Author(s):  
A Parket ◽  
M Kupiec
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Uv Irradiation ◽  
Mitotic Recombination ◽  
Methyl Methanesulfonate ◽  
Ectopic Recombination ◽  
Ty Elements ◽  
Chemical Agents ◽  
Physical And Chemical

Mitotic recombination is increased when cells are treated with a variety of physical and chemical agents that cause damage to their DNA. We show here, using Saccharomyces cerevisiae strains that carry marked Ty elements, that recombination between members of this family of retrotransposons is not increased by UV irradiation or by treatment with the radiomimetic drug methyl methanesulfonate. Both ectopic recombination and mutation events were elevated by these agents for non-Ty sequences in the same strain. We discuss possible mechanisms that can prevent the induction of recombination between Ty elements.

scholarly journals Postreplication Repair and PCNA Modification in Schizosaccharomyces pombe

2006 ◽  
Vol 17 (7) ◽  
pp. 2976-2985 ◽  
Author(s):  
Jonathan Frampton ◽  
Anja Irmisch ◽  
Catherine M. Green ◽  
Andrea Neiss ◽  
Michelle Trickey ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Ionizing Radiation ◽  
Schizosaccharomyces Pombe ◽  
Uv Irradiation ◽  
Proliferating Cell Nuclear Antigen ◽  
S Phase ◽  
Cell Cycle Checkpoints ◽  
Nuclear Antigen ◽  
Proliferating Cell

Ubiquitination of proliferating cell nuclear antigen (PCNA) plays a crucial role in regulating replication past DNA damage in eukaryotes, but the detailed mechanisms appear to vary in different organisms. We have examined the modification of PCNA in Schizosaccharomyces pombe. We find that, in response to UV irradiation, PCNA is mono- and poly-ubiquitinated in a manner similar to that in Saccharomyces cerevisiae. However in undamaged Schizosaccharomyces pombe cells, PCNA is ubiquitinated in S phase, whereas in S. cerevisiae it is sumoylated. Furthermore we find that, unlike in S. cerevisiae, mutants defective in ubiquitination of PCNA are also sensitive to ionizing radiation, and PCNA is ubiquitinated after exposure of cells to ionizing radiation, in a manner similar to the response to UV-irradiation. We show that PCNA modification and cell cycle checkpoints represent two independent signals in response to DNA damage. Finally, we unexpectedly find that PCNA is ubiquitinated in response to DNA damage when cells are arrested in G2.

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