scholarly journals Long-Range PCR and Next-Generation Sequencing of BRCA1 and BRCA2 in Breast Cancer

2012 ◽  
Vol 14 (5) ◽  
pp. 467-475 ◽  
Author(s):  
Hilmi Ozcelik ◽  
Xuejiang Shi ◽  
Martin C. Chang ◽  
Eric Tram ◽  
Matt Vlasschaert ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Next Generation Sequencing ◽  
Long Range ◽  
Next Generation ◽  
Brca1 And Brca2 ◽  
Long Range Pcr ◽  
Generation Sequencing

scholarly journals Detection of Genomic Variations in BRCA1 and BRCA2 Genes by Long-Range PCR and Next-Generation Sequencing

2012 ◽  
Vol 14 (3) ◽  
pp. 286-293 ◽  
Author(s):  
Imma Hernan ◽  
Emma Borràs ◽  
Miguel de Sousa Dias ◽  
María José Gamundi ◽  
Begoña Mañé ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Long Range ◽  
Next Generation ◽  
Brca1 And Brca2 ◽  
Genomic Variations ◽  
Brca1 And Brca2 Genes ◽  
Long Range Pcr ◽  
Generation Sequencing

scholarly journals Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e28240 ◽  
Author(s):  
Ellen Knierim ◽  
Barbara Lucke ◽  
Jana Marie Schwarz ◽  
Markus Schuelke ◽  
Dominik Seelow
Keyword(s):  
Next Generation Sequencing ◽  
Long Range ◽  
Next Generation ◽  
Systematic Comparison ◽  
Pcr Products ◽  
Long Range Pcr ◽  
Generation Sequencing

scholarly journals Analysis of the pathogenic variants of BRCA1 and BRCA2 using next-generation sequencing in women with familial breast cancer: a case–control study

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Omar Alejandro Zayas-Villanueva ◽  
Luis Daniel Campos-Acevedo ◽  
José de Jesús Lugo-Trampe ◽  
David Hernández-Barajas ◽  
Juan Francisco González-Guerrero ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Next Generation Sequencing ◽  
Familial Breast Cancer ◽  
Case Control Study ◽  
Case Control ◽  
Next Generation ◽  
Brca1 And Brca2 ◽  
Pathogenic Variants ◽  
Generation Sequencing ◽  
Control Study

scholarly journals Identification of BRCA1/2 mutations in breast cancer patients by next-generation sequencing

2018 ◽  
pp. 168-173
Author(s):  
I.F. Stetsenko ◽  
A.Yu. Krasnenko ◽  
U.S. Stanoevich ◽  
A.A. Mescheryakov ◽  
I.K. Vorotnikov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Next Generation Sequencing ◽  
Cancer Patients ◽  
Clinical Test ◽  
Next Generation ◽  
Breast Cancer Patients ◽  
Post Translational Modification ◽  
Brca1 And Brca2 ◽  
Cancer Pathogenesis ◽  
Generation Sequencing

Breast cancer is one of the most widespread forms of solid tumors. By analyzing the traits of breast cancer pathogenesis at the molecular level using modern genetic analysis techniques and at different stages of the disease new data can be obtained to be further utilized in clinical practice. Molecular profiling based on next-generation sequencing is being increasingly applied as a clinical test to select target drugs for treating breast cancer patients with tumors highly resistant to therapy. In this study, we performed targeted sequencing of BRCA1 and BRCA2 oncogenes. In the total of 66 DNA samples from patients with breast tumors, BRCA1/2 mutations were found in 39 patients. There were 78 unique genetic variants, including 30 mutations in BRCA1 and 48 mutations in BRCA2. We identified 33 mutations affecting the sites of post-translational modification in proteins (PMT mutations).

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