Double labeling serial sections to enhance three-dimensional imaging of injured spinal cord

2004 ◽  
Vol 134 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Bradley S. Duerstock
Keyword(s):  
Spinal Cord ◽  
Three Dimensional ◽  
Injured Spinal Cord ◽  
Serial Sections ◽  
Double Labeling ◽  
Three Dimensional Imaging

scholarly journals X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury

2015 ◽  
Vol 22 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Kenta Takashima ◽  
Masato Hoshino ◽  
Kentaro Uesugi ◽  
Naoto Yagi ◽  
Shojiro Matsuda ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Spinal Cord ◽  
Tissue Engineering ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Injured Spinal Cord ◽  
X Ray ◽  
Biodegradable Scaffolds ◽  
Cord Injury ◽  
Grating Interferometer

Tissue engineering strategies for spinal cord repair are a primary focus of translational medicine after spinal cord injury (SCI). Many tissue engineering strategies employ three-dimensional scaffolds, which are made of biodegradable materials and have microstructure incorporated with viable cells and bioactive molecules to promote new tissue generation and functional recovery after SCI. It is therefore important to develop an imaging system that visualizes both the microstructure of three-dimensional scaffolds and their degradation process after SCI. Here, X-ray phase-contrast computed tomography imaging based on the Talbot grating interferometer is described and it is shown how it can visualize the polyglycolic acid scaffold, including its microfibres, after implantation into the injured spinal cord. Furthermore, X-ray phase-contrast computed tomography images revealed that degradation occurred from the end to the centre of the braided scaffold in the 28 days after implantation into the injured spinal cord. The present report provides the first demonstration of an imaging technique that visualizes both the microstructure and degradation of biodegradable scaffolds in SCI research. X-ray phase-contrast imaging based on the Talbot grating interferometer is a versatile technique that can be used for a broad range of preclinical applications in tissue engineering strategies.

Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury

2011 ◽  
Vol 18 (1) ◽  
pp. 166-171 ◽  
Author(s):  
Ali Ertürk ◽  
Christoph P Mauch ◽  
Farida Hellal ◽  
Friedrich Förstner ◽  
Tara Keck ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Axon Regeneration ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Adult Spinal Cord

High-Spatial-Resolution Three-dimensional Imaging of Human Spinal Cord and Column Anatomy with Postmortem X-ray Phase-Contrast Micro-CT

Radiology ◽  
2021 ◽  
Vol 298 (1) ◽  
pp. 135-146
Author(s):  
Giacomo E. Barbone ◽  
Alberto Bravin ◽  
Alberto Mittone ◽  
Sergio Grosu ◽  
Jens Ricke ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
High Spatial Resolution ◽  
Three Dimensional ◽  
Micro Ct ◽  
Three Dimensional Imaging ◽  
X Ray ◽  
Human Spinal Cord

scholarly journals Three-dimensional imaging of microvasculature in the rat spinal cord following injury

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yong Cao ◽  
Tianding Wu ◽  
Zhou yuan ◽  
Dongzhe Li ◽  
Shuangfei Ni ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Three Dimensional ◽  
Rat Spinal Cord ◽  
Three Dimensional Imaging

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

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